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121.
Shahsavandi S Salmanian AH Ghorashi SA Masoudi S Fotouhi F Ebrahimi MM 《Molecular biology reports》2011,38(5):3293-3298
Influenza A viruses are subtyped according to antigen characterization of hemagglutinin (HA) and neuraminidase surface glycoproteins.
The hemagglutination inhibition (HI) assay using reference antiserum is currently applied to serologic screening of subtype-specific
antibodies in sera. The reference antiserum is made by injecting chickens with live or inactivated whole virus preparations.
Nonspecific inhibitors of antisera prepared by the conventional method may affect the specificity of HI assay. In this study,
highly pure recombinant proteins generated using baculovirus expression vector system based on full-length of HA (HAF) and
antigenic region of HA1 genes of H9 subtype, and also inactivated whole virus were used to immunization of chickens. Measurable antibody titers were
present for treated birds after 3 weeks and generally increased after each boost. The performance of the prepared antisera
was evaluated by testing a panel of known standard strains of influenza virus representing five HA subtypes. Relative to the
conventional method using whole virus immunization and recombinant HAF protein, the antiserum prepared by recombinant HA1 had a specificity of 100% for all tested subtypes. The antiserum prepared by expression of HA1 protein in baculovirus has
the potential for rapid and specific HA subtyping of influenza viruses without producing antibodies specific to other viral
proteins. 相似文献
122.
Felipe A. de Oliveira Mohamed H. Shahin Yan Gong Caitrin W. McDonough Amber L. Beitelshees John G. Gums Arlene B. Chapman Eric Boerwinkle Stephen T. Turner Reginald F. Frye Oliver Fiehn Rima Kaddurah-Daouk Julie A. Johnson Rhonda M. Cooper-DeHoff 《Metabolomics : Official journal of the Metabolomic Society》2016,12(8):129
Introduction
While atenolol is an effective antihypertensive agent, its use is also associated with adverse events including hyperglycemia and incident diabetes that may offset the benefits of blood pressure lowering. By combining metabolomic and genomic data acquired from hypertensive individuals treated with atenolol, it may be possible to better understand the pathways that most impact the development of an adverse glycemic state.Objective
To identify biomarkers that can help predict susceptibility to blood glucose excursions during exposure to atenolol.Methods
Plasma samples acquired from 234 Caucasian participants treated with atenolol in the Pharmacogenomic Evaluation of Antihypertensive Responses trial were analyzed by gas chromatography Time-Of-Flight Mass Spectroscopy. Metabolomics and genomics data were integrated by first correlating participant’s metabolomic profiles to change in glucose after treatment with atenolol, and then incorporating genotype information from genes involved in metabolite pathways associated with glucose response.Results
Our findings indicate that the baseline level of β-alanine was associated with glucose change after treatment with atenolol (Q = 0.007, β = 2.97 mg/dL). Analysis of genomic data revealed that carriers of the G allele for SNP rs2669429 in gene DPYS, which codes for dihydropyrimidinase, an enzyme involved in β-alanine formation, had significantly higher glucose levels after treatment with atenolol when compared with non-carriers (Q = 0.05, β = 2.76 mg/dL). This finding was replicated in participants who received atenolol as an add-on therapy (P = 0.04, β = 1.86 mg/dL).Conclusion
These results suggest that β-alanine and rs2669429 may be predictors of atenolol-induced hyperglycemia in Caucasian individuals and further investigation is warranted.123.
Isabelle Menez Christian Gespach Shahin Emami Gabriel Rosselin 《Biochemical and biophysical research communications》1983,116(1):251-257
We compared the interaction of AH 22216 (a new histamine H2 receptor antagonist) and cimetidine on the receptor-cAMP systems sensitive to histamine and to Vasoactive Intestinal Peptide (VIP) in the human gastric cancer cell line HGT-1. When added simultaneously with histamine (10?4 M), the potency of AH 22216 is similar to that of cimetidine (IC50 = 4–6.6×10?6 M, respectively). Schild plot analysis indicated a non-competitive inhibition by AH 22216 (pA2=6.22, slope=1.4±0.03). Preincubations of AH 22216 (10 min, 10?5 M) with HGT-1 cells (even after a washout period) resulted in a complete and persistent (60 min) inactivation of the subsequent histamine effect, without changing the kinetics of the VIP-induced stimulation in the system. Under these conditions, the potency of AH 22216 increased from 6.6 to 0.7 × 10?6 M. This inactivation was not observed with cimetidine. The data indicate that AH 22216 is an irreversible and specific inhibitor of the gastric histamine H2 receptor. 相似文献
124.
M M Shahin 《Mutation research》1975,30(2):191-198
Niridazole, one of several drugs presently known to be of value in the treatment of human schistosomiasis, was tested for its activity in inducing mitotic recombination in yeast. It was found that niridazole is genetically active when the treatment of yeast cells is performed in a rich medium (YPG-medium) under growing conditions, but not when treatment is carried out in a non-nutrient suspension (phosphate buffer). The data suggest that niridazole might be converted to an active compound by yeast metabolism. The results of the experiments with niridazole in the non-nutrient medium were compared with those of AF-2 and SQ18, 506, two agents which have been shown to be genetically active in the present assay system. 相似文献
125.
5 different histidine-requiring strains of Salmonella typhimurium were used to test the mutagenic activity of 7 different fractions of Athabasca tar-sand. None of the 7 fractions (bitumen, maltenes, asphaltenes, saturated, monoaromatic, diaromatic and polyaromatic hydrocarbons), showed positive mutagenic response in any of the Salmonella typhimurium strains. We have tested a wide range of concentrations. The results obtained so far are consistent with the lack of mutagenic activity of all investigated fractions in the absence and in the presence of metabolic activation. Assuming that there might be an association between the absence of mutagenic activity and the complexity of the tar-sand fractions, we investigated the effect of the polyaromatic hydrocaron fraction on the mutagenicity of the carcinogenic agent 2-aminoanthracene. The data obtained indicate clearly that the polyaromatic hydrocarbon fraction suppresses the mutagenic activity of 2-aminoanthracene. 相似文献
126.
127.
F.?C.?HowarthEmail author A.?Qureshi A.?Shahin M.?L.?Lukic 《Molecular and cellular biochemistry》2005,269(1):103-108
Administration of a single high-dose (SHD) of streptozotocin (STZ) to young adult rats causes a diabetic cardiomyopathy. Albino Oxford (AO) and Dark Agouti (DA) inbred strains of rats are susceptible to developing diabetes when administered a SHD of STZ but differ in susceptibility to multiple low-dose (MLD) STZ. We have investigated the effects of SHD and MLD-STZ on contraction and intracellular Ca2+, measured with fura-2, in ventricular myocytes from AO and DA rats at 18–20 weeks after treatment. Time to peak shortening was significantly prolonged in myocytes from DA rats after SHD-STZ but was not altered in DA rats after MLD-STZ or in AO rats by either MLD or SHZ-STZ treatment. Time to peak shortening in myocytes from DA control and DA rats after SHD-STZ were 88 ± 2 ms and 107 ± 4 ms, respectively. Time to half relaxation and the amplitude of myocyte shortening were not altered in AO or DA rats by either MLD or SHD-STZ treatment. Amplitude, time to peak fura-2 transient and time to half relaxation of the fura-2 transient were not significantly altered in AO or DA rats by either MLD or SHD-STZ treatment. Contractile defects reported in myocytes from SHD-STZ treated DA rats may be a consequence of altered myofilament sensitivity to Ca2+. The hyperglycaemic effects of MLD-STZ and SHD-STZ induced diabetes was much greater in DA compared to AO rats and the effects of the hyperglycaemia on the time-course of ventricular myocyte contraction was most profound in DA rats after SHD-STZ. (Mol Cell Biochem 269: 103–108, 2005) 相似文献
128.
Trefoil factor family (TFF) peptides and cancer progression 总被引:6,自引:0,他引:6
Emami S Rodrigues S Rodrigue CM Le Floch N Rivat C Attoub S Bruyneel E Gespach C 《Peptides》2004,25(5):885-898
TFF peptides are involved in mucosal maintenance and repair through motogenic and antiapoptotic activities. These peptides are overexpressed during inflammatory processes and cancer progression. They also function as scatter factors, proinvasive and angiogenic agents. Such a divergence is related to the pathophysiological state of tissues submitted to persistent aggressive situations during digestive processes in the normal gastrointestinal tract, inflammatory and neoplastic diseases. In agreement with this model, TFF peptides are connected with multiple oncogenic pathways. As a consequence, the TFF signaling pathways may serve as potential targets in the control of chronic inflammation and progression of human solid tumors. 相似文献
129.
Saeed Nouri Jamshid Ghiasi Ghalehkandi Shahin Hassanpour Habib Aghdam-Shahryar 《International journal of peptide research and therapeutics》2018,24(3):463-470
This study designed to determine effect of in ovo feeding of folic acid on subsequent growth performance and blood constituents levels in broilers. A total of 1000 fertile broiler eggs were divided into four groups. Control group (1) received no injection. In group 2, eggs received in ovo feeding of distiller water (40 µg). Group 3 received in ovo feeding of folic acid (40 µg). Groups 4 and 5 were similar to Group 3, except eggs injected with 80 and 120 µg of folic acid. All eggs were incubated and after hatch chickens were randomly assigned into their experimental groups. On days 1 and 42 post-hatch, chicken randomly selected and blood constituents, carcass characteristics, food intake, body weight gain and food conversion ratio were determined. According to the results, no significant difference detected on hatchability rate of the in ovo injected eggs (P?>?0.05). Dose dependent increase observed in glucose and folic acid levels in chicken in ovo injected with folic acid on day 1 post hatch (P?=?0.001). Blood glucose, folic acid and phosphorous levels increased (P?=?0.001) while cholesterol, triglyceride, HDL and LDL, calcium and alkaline phosphatase decreased in ovo injected with folic acid on day 42 post hatch (P?=?0.001). Food conversion ratio increased by in ovo injection of the folic acid (P?=?0.001). These results suggest folic acid had positive effects in broiler chicken. 相似文献
130.
Shahin Emami Wilma Merrill Van Cherington Gisela G. Chiang Michael Kirchgesser Joseph M. Appel Michael Hansen Peter H. Levine Joel S. Greenberger David R. Hurwitz 《In vitro cellular & developmental biology. Animal》1997,33(7):503-511
Summary The ex vivo establishment, expansion, transduction, and reintroduction of autologous bone marrow stromal cells offers a potential efficacious
system for somatic cell gene therapy. It is likely that any ex vivo system will require the use of large numbers of cells which express high levels of transgene products. We present a method
for routine expansion of canine bone marrow stromal cells, established from initial 10–20 ml marrow aspirates, to greater
than 109 cells. This high level expansion of cell cultures uses the stimulatory effect of acidic fibroblast growth factor (aFGF) and
heparin. In the absence of these factors, stromal cell cultures grow actively for only 1 to 2 passages, become flattened in
morphology, and expand to only 108 cells. In the presence of heparin (5 U/ml), aFGF exerts its effect over a wide range of concentrations (0.1–10 ng/ml) in
a dose-dependent manner. The stimulatory effect is dependent on the presence of both aFGF and heparin. Immunocytochemical
and cytochemical analyses phenotypically characterize these stromal cells as bone marrow stromal myofibroblasts. Stromal cells
grown in the presence of aFGF and heparin grow actively and maintain a fibroblast-like morphology for a number of passages,
transduce efficiently with a human growth hormone (hGH) expression vector, and express and secrete high levels of hGH. Human
marrow stromal cells were also established and expanded by the same culture method. This culture method should be of great
value in somatic cell gene therapy for the delivery of secreted gene products to the plasma of large mammals. 相似文献