全文获取类型
收费全文 | 1875篇 |
免费 | 145篇 |
国内免费 | 2篇 |
出版年
2023年 | 20篇 |
2022年 | 50篇 |
2021年 | 72篇 |
2020年 | 44篇 |
2019年 | 43篇 |
2018年 | 87篇 |
2017年 | 53篇 |
2016年 | 59篇 |
2015年 | 93篇 |
2014年 | 101篇 |
2013年 | 129篇 |
2012年 | 126篇 |
2011年 | 121篇 |
2010年 | 71篇 |
2009年 | 69篇 |
2008年 | 71篇 |
2007年 | 67篇 |
2006年 | 54篇 |
2005年 | 59篇 |
2004年 | 78篇 |
2003年 | 55篇 |
2002年 | 41篇 |
2001年 | 35篇 |
2000年 | 44篇 |
1999年 | 26篇 |
1998年 | 13篇 |
1997年 | 14篇 |
1996年 | 11篇 |
1995年 | 12篇 |
1994年 | 10篇 |
1993年 | 12篇 |
1992年 | 27篇 |
1991年 | 8篇 |
1990年 | 15篇 |
1989年 | 20篇 |
1988年 | 16篇 |
1987年 | 16篇 |
1986年 | 9篇 |
1985年 | 11篇 |
1984年 | 9篇 |
1982年 | 8篇 |
1981年 | 9篇 |
1980年 | 14篇 |
1979年 | 19篇 |
1978年 | 8篇 |
1976年 | 8篇 |
1975年 | 11篇 |
1973年 | 9篇 |
1971年 | 14篇 |
1970年 | 8篇 |
排序方式: 共有2022条查询结果,搜索用时 15 毫秒
101.
102.
Siddiqui Rehan Ahmed Simjee Shabana Usman Kabir Nurul Ateeq Muhammad Shah M. Raza Hussain Syed Saad 《Molecular and cellular biochemistry》2019,450(1-2):43-52
Molecular and Cellular Biochemistry - The protective activity of N-(2-hydroxyphenyl)acetamide (NA-2) and NA-2-coated gold nanoparticles (NA-2-AuNPs) in glycerol-treated model of acute kidney injury... 相似文献
103.
Mohd Faizan Siddiqui Mohd Shahnawaz Khan Fohad Mabood Husain 《Journal of biomolecular structure & dynamics》2019,37(9):2230-2241
Carbendazim is a benzimidazole fungicide used to control the fungal invasion. However, its exposure might lead to potential health problems. The present study evaluates the interaction of carbendazim (CAR) with human serum albumin (HSA) which is an important drug carrier protein and plays a very crucial role in the transportation of small molecules. A number of biophysical techniques were employed to investigate the binding of CAR with HSA. The increased UV-absorption of HSA on titrating with CAR suggests the formation of HSA–CAR complex and it could be due to the exposure of aromatic residues. The fluorescence study confirmed that CAR quenches the fluorescence of HSA and showed the static mode of quenching. CAR (50 µM) quenches around 56.14% of the HSA fluorescence. The quenching constant, binding constant, number of binding site and free energy change was calculated by fluorescence quenching experiment. Competitive displacement assay showed Sudlow’s site I as the primary binding site of CAR on HSA. The synchronous fluorescence study revealed the perturbation in the microenvironment around tyrosine and tryptophan residues upon binding of CAR to HSA. The circular dichroism results suggested that the binding of CAR to HSA altered its secondary structure. Molecular docking experiment demonstrated the binding of CAR to Sudlow’s site I of HSA. Docking studies suggested that the hydrogen bonding, van der Waals and pi-alkyl are playing role in the interaction of CAR with HSA. The study confirmed the conformational changes within HSA upon binding of CAR. 相似文献
104.
105.
Faizan Q. Siddiqui Farnaz Malik F. R. Y. Fazli 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1995,666(2)
A rapid, sensitive and specific high-performance liquid chromatographic method was developed for the determination of serum levels of retinol in humans. A direct serum injection technique after deproteinisation was used to avoid lengthy pretreatment steps which can result in degradation of retinol during analysis. The column used was CLC-ODS, the mobile phase was acetonitrile-water and detection wavelength was 328 nm. Deterioration in column performance was not observed even after injection of 300 samples. The lower detection limit was 10 μg/l. On analyzing a serum pool six times, a C.V of 0.7% was obtained. The method is quantitative, reproducible, rapid and highly accurate for routine analysis. 相似文献
106.
Synthesis of lactoferrin and casein by the bovine mammary gland was determined in an experimental model where lactation was maintained in one mammary half, while involution was induced in the contralateral half. Culture of explants with prolactin had no consistent effect on synthesis of casein or lactoferrin in tissue from either mammary half. Endotoxin and tumor necrosis factor-α generally decreased synthesis of casein and lactoferrin, suggesting that these inflammatory mediators are not directly responsible for increasing lactoferrin synthesis during mammary inflammation or involution. Synthesis of lactoferrin was increased and casein decreased in the involuting mammary half vs. the lactating half. These results suggest that local factors in the mammary gland play a role in the regulation of lactoferrin synthesis during involution. 相似文献
107.
Analysis of the relative amount and distribution pattern of phloem sclerenchyma in some 29 tropical trees of India was carried out with systematic considerations. Both the features exhibited considerable diversity among the species analyzed. The extent of area occupied by sclerenchyma in transections of the bark of these species varied from zero to 35% and 3 to 49% in the conducting and non-conducting phloem zones respectively, the overall amount constituting about 3 to 45% of the secondary phloem as a whole. The sclerenchyma cells differentiated either as isolated elements or in the form of bands or fascicles of diverse shape and size showing various distribution patterns. 相似文献
108.
Conformational searches on three closely related pp60(c-src) protein tyrosine kinase inhibitors of varying potencies were performed to determine a structural basis for their activity. The first was a linear peptide (PDNEYAFFQf), the second its 10-membered cyclic analogue, and the third a cyclic analogue with a para carboxyphenylalanine in place of one the F residues. A common backbone conformation with an antiparallel beta-sheet-like geometry capped by similar beta-turns was found for all three peptides, which may be a binding conformation and gives a candidate pharmacophore for further testing. The interaction between some polar side chains and between some of the aromatic rings may be important for maintaining the correct conformation. The differences in potencies of these inhibitors may be attributed to certain thermodynamic and chemical reasons. 相似文献
109.
Curzen N Khawaja S Rothman M 《International journal of cardiovascular interventions》1999,2(2):117-119
We describe the treatment of a bifurcation lesion at the anastomosis of a vein graft by 'true Y' stenting which preserved both antegrade and retrograde flow away from the graft. 相似文献
110.