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91.
Signals derived from YABBY gene activities in organ primordia regulate growth and partitioning of Arabidopsis shoot apical meristems 总被引:1,自引:0,他引:1
Shoot apical meristems (SAMs) are self-sustaining groups of cells responsible for the ordered initiation of all aerial plant tissues, including stems and lateral organs. The precise coordination of these processes argues for crosstalk between the different SAM domains. The products of YABBY (YAB) genes are limited to the organ primordium domains, which are situated at the periphery of all SAMs and which are separated by a margin of three to seven cells from the central meristem zone marked by WUSCHEL and CLAVATA3 expression. Mutations in the two related YAB1 genes, FILAMENTOUS FLOWER and YABBY3 (YAB3), cause an array of defects, including aberrant phyllotaxis. We show that peripheral YAB1 activity nonautonomously and sequentially affects the phyllotaxis and growth of subsequent primordia and coordinates the expression of SAM central zone markers. These effects support a role for YAB1 genes in short-range signaling. However, no evidence was found that YAB1 gene products are themselves mobile. A screen for suppression of a floral YAB1 overexpression phenotype revealed that the YAB1-born signals are mediated in part by the activity of LATERAL SUPPRESSOR. This GRAS protein is expressed at the boundary of organ primordia and the SAM central zone, distinct from the YAB1 expression domain. Together, these results suggest that YAB1 activity stimulates signals from the organs to the meristem via a secondary message or signal cascade, a process essential for organized growth of the SAM. 相似文献
92.
93.
Mapping of Ve in tomato: a gene conferring resistance to the broad-spectrum pathogen, Verticillium dahliae race 1 总被引:3,自引:0,他引:3
N. Diwan R. Fluhr Y. Eshed D. Zamir S. D. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(2):315-319
The soil-borne fungi Verticillium spp. cause vascular wilt disease in a wide range of crop plants. In tomato, resistance to Verticillium dahliae race 1 is conferred by a single dominant gene, Ve. Previous efforts to map Ve in tomato have yielded confusing results, locating it on different chromosomes, which subsequently raised the possibility
that Verticillium resistance may be controlled by a number of loci. We used three different mapping populations to obtain an unambiguous map
location of Ve: a recombinant inbred (RI) line population; an F2 population segregating for Verticillium resistance; and a population of 50 introgression lines (IL). In all of the mapping populations Ve was positioned on the short arm of chromosome 9 tightly linked to the RFLP marker GP39. This linkage was confirmed by screening for GP39 in different breeding lines with known resistance or susceptibility to Verticillium. A perfect match was found between GP39 and the Verticillium response of the lines, indicating the potential of GP39 in the rapid detection of Verticillium resistance and as a starting point for map-based cloning of Ve. This approach is particularly relevant for Verticillium dahliae race 1, since in the present work we also show that the isolate that infects tomato is responsible for wilt disease in other
important crop plants.
Received: 5 July 1998 / Accepted: 28 July 1998 相似文献
94.
Songlei Liu Sukanya Punthambaker Eswar P R Iyer Thomas Ferrante Daniel Goodwin Daniel Fürth Andrew C Pawlowski Kunal Jindal Jenny M Tam Lauren Mifflin Shahar Alon Anubhav Sinha Asmamaw T Wassie Fei Chen Anne Cheng Valerie Willocq Katharina Meyer King-Hwa Ling Conor
K Camplisson Richie E Kohman John Aach Je Hyuk Lee Bruce
A Yankner Edward S Boyden George M Church 《Nucleic acids research》2021,49(10):e58
95.
Overripe ova and twinning. 总被引:4,自引:2,他引:2
Multiple births were studied in a sample of orthodox Jewesses for whom an estimate could be made of the day of ovulation and the earliest possible day of conception. The overall rate of twinning was 14.5/1,000 deliveries, and of triplets, 0.40/1,000. Twinning rates varied significantly from 11.4 in the 5,976 "early" conceptions (day -1 or earlier relative to the estimated day of ovulation) to 26.9 in the 1,498 "late" conceptions (day 0 or later). Triplets varied significantly from 0 to 2.01 in early and late conceptions, respectively, and unlike-sexed multiple sets, 2.8 and 12.8, respectively. The excess of multiple births in late conceptions was seen within different ages and origin groups, in women with different menstrual characteristics, and in those with and without treatment for anovulation. While the excess of unlike-sexed sets seems to lead to the conclusion that late conceptions are associated with dizygosity, polar body twinning and uniovular dispermatic twinning should also be considered. 相似文献
96.
97.
The Transient Receptor Potential (TRP) proteins constitute a large and diverse family of channel proteins, which is conserved through evolution. TRP channel proteins have critical functions in many tissues and cell types, but their gating mechanism is an enigma. In the present study patch-clamp whole-cell recordings was applied to measure the TRP- and TRP-like (TRPL)-dependent currents in isolated Drosophila ommatidia. Also, voltage responses to light and to metabolic stress were recorded from the eye in vivo. We report new insight into the gating of the Drosophila light-sensitive TRP and TRPL channels, by which both Ca2+ and protein dephosphorylation are required for channel activation. ATP depletion or inhibition of protein kinase C activated the TRP channels, while photo-release of caged ATP or application of phorbol ester antagonized channels openings in the dark. Furthermore, Mg(2+)-dependent stable phosphorylation event by ATPgammaS or protein phosphatase inhibition by calyculin A abolished activation of the TRP and TRPL channels. While a high reduction of cellular Ca2+ abolished channel activation, subsequent application of Ca2+ combined with ATP depletion induced a robust dark current that was reminiscent of light responses. The results suggest that the combined action of Ca2+ and protein dephosphorylation activate the TRP and TRPL channels, while protein phosphorylation by PKC antagonized channels openings. 相似文献
98.
An Introgression Line Population of Lycopersicon Pennellii in the Cultivated Tomato Enables the Identification and Fine Mapping of Yield-Associated Qtl 总被引:60,自引:1,他引:59
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Methodologies for mapping of genes underlying quantitative traits have advanced considerably but have not been accompanied by a parallel development of new population structures. We present a novel population consisting of 50 introgression lines (ILs) originating from a cross between the green-fruited species Lycopersicon pennellii and the cultivated tomato (cv M82). Each of the lines contains a single homozygous restriction fragment length polymorphism-defined L. pennellii chromosome segment, and together the lines provide complete coverage of the genome and a set of lines nearly isogenic to M82. A field trial of the ILs and their hybrids revealed at least 23 quantitative trait loci (QTL) for total soluble solids content and 18 for fruit mass; these estimates are twice as high as previously reported estimates based on traditional mapping populations. For finer mapping of a QTL affecting fruit mass, the introgressed segment was recombined into smaller fragments that allowed the identification of three linked loci. At least 16 QTL for plant weight, 22 for percentage green fruit weight, 11 for total yield and 14 for total soluble solids yield were identified. Gene action for fruit and plant characteristics was mainly additive, while overdominance (or pseudo-overdominance) of wild species introgressions was detected for yield. 相似文献
99.
Yaffa Mizrachi Nebenzahl Karin Blau Tatyana Kushnir Marilou Shagan Maxim Portnoi Aviad Cohen Shalhevet Azriel Itai Malka Asad Adawi Daniel Kafka Shahar Dotan Gali Guterman Shany Troib Tali Fishilevich Jonathan M Gershoni Alex Braiman Andrea M Mitchell Timothy J Mitchell Nurith Porat Inna Goliand Vered Chalifa Caspi Edwin Swiatlo Michael Tal Ronald Ellis Natalie Elia Ron Dagan 《PloS one》2016,11(3)
In Streptococcus pneumonia, phosphoenolpyruvate protein phosphotransferase (PtsA) is an intracellular protein of the monosaccharide phosphotransferase systems. Biochemical and immunostaining methods were applied to show that PtsA also localizes to the bacterial cell-wall. Thus, it was suspected that PtsA has functions other than its main cytoplasmic enzymatic role. Indeed, recombinant PtsA and anti-rPtsA antiserum were shown to inhibit adhesion of S. pneumoniae to cultured human lung adenocarcinoma A549 cells. Screening of a combinatorial peptide library expressed in a filamentous phage with rPtsA identified epitopes that were capable of inhibiting S. pneumoniae adhesion to A549 cells. The insert peptides in the phages were sequenced, and homologous sequences were found in human BMPER, multimerin1, protocadherin19, integrinβ4, epsin1 and collagen type VIIα1 proteins, all of which can be found in A549 cells except the latter. Six peptides, synthesized according to the homologous sequences in the human proteins, specifically bound rPtsA in the micromolar range and significantly inhibited pneumococcal adhesion in vitro to lung- and tracheal-derived cell lines. In addition, the tested peptides inhibited lung colonization after intranasal inoculation of mice with S. pneumoniae. Immunization with rPtsA protected the mice against a sublethal intranasal and a lethal intravenous pneumococcal challenge. In addition, mouse anti rPtsA antiserum reduced bacterial virulence in the intravenous inoculation mouse model. These findings showed that the surface-localized PtsA functions as an adhesin, PtsA binding peptides derived from its putative target molecules can be considered for future development of therapeutics, and rPtsA should be regarded as a candidate for vaccine development. 相似文献
100.
Anat Hendelman Sophia Zebell Daniel Rodriguez-Leal Noah Dukler Gina Robitaille Xuelin Wu Jamie Kostyun Lior Tal Peipei Wang Madelaine E. Bartlett Yuval Eshed Idan Efroni Zachary B. Lippman 《Cell》2021,184(7):1724-1739.e16
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