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181.
Bragg W Norton D Shamsi SA 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2008,875(1):304-316
This work focuses on the simultaneous analysis of beta-blockers with multiple stereogenic centers using capillary electrochromatography-mass spectrometry (CEC-MS) with a vancomycin stationary phase. The critical mobile phase variables (composition of organic solvents, acid/base ratios) as well as column temperature and electric field strength, effecting enantioresolution and analysis time were first optimized sequentially. Next, to achieve global optimum a multivariate D-optimal design was used. Although multivariate approach did not improve enantioresolution any further, analysis time was significantly reduced. Under optimum CEC-MS conditions, all stereoisomers were resolved with resolution in the range 1.0-3.1 in less than 60 min with an average signal-to-noise (S/N) greater than 1000. The developed CEC-MS method has the potential to emerge as a screening method for analysis of multiple chiral compounds using a single protocol using the same column and mobile phase conditions, thus reducing the operation time and cost. 相似文献
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Sephadex and sephadex ion-exchange filtration improves the quality and freezability of low-grade buffalo semen ejaculates 总被引:3,自引:0,他引:3
The effect of sephadex and sephadex ion-exchange filtration on the improvement in quality and freezability of low-grade buffalo semen ejaculates was assessed. Two types of filtration columns were used: one containing only sephadex G-10 (FS) and the other sephadex G-10 along with ion-exchangers (diethyl amino ethane-52 (DEAE-52) cellulose and carboxy methyl-52 (CM-52) cellulose; FS+IE). Unfiltered samples served as controls. Semen ejaculates extended in Tris-citric acid (1:4) (n=16; initial motility 40-50%) were filtered at the rate of 1.5 ml/min under negative pressure at room temperature (28-30 degrees C). The mean recovery rate (%) of motile spermatozoa in the FS (85.9+/-1.51) and FS+IE (77.10+/-2.28) filtrates did not differ significantly. Percentages of sperm motility, normal acrosomes, and intact plasma membranes were highest (P<0.05) in FS+IE, intermediate (P<0.05) in FS and lowest (P<0.05) in controls at the three stages of cryopreservation (postfiltration final dilution, after equilibration, and after freezing). Mean sperm abnormalities were lowest (P<0.05) in the filtrates of FS+IE, moderate (P<0.05) in FS and highest in controls at all stages of freezing. Compared to dilution and equilibration, freezing greatly reduced (P<0.05) the overall percent motility, normal acrosomes and intact plasma membranes. The spermatozoa eluted through FS+IE columns proved more resistant (P<0.05) in bearing dilution, equilibration, freezing and thawing stresses than the spermatozoa from FS and control samples. It is concluded that filtration systems containing an FS+IE column can effectively enhance the quality and freezability of extended, low quality buffalo semen. 相似文献
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185.
Interferons regulate a number of biological functions including control of cell proliferation, generation of antiviral activities and immumodulation in human cells. Studies by several investigators have identified a number of cellular signaling cascades that are activated during engagement of interferon receptors. The activation of multiple signaling cascades by the interferon receptors appears to be critical for the generation of interferon-mediated biological functions and immune surveillance. The present review summarizes the existing knowledge on the multiple signaling cascades activated by Type I interferons. Recent developments in this research area are emphasized and the implications of these new discoveries on our understanding of interferon actions are discussed. 相似文献
186.
Yong Tang Richard P. Chandra Shahab Sokhansanj Jack N. Saddler 《Bioenergy Research》2018,11(2):341-350
Steam treatment has been reported to improve the durability of wood pellet likely by changing the physical and chemical structures of wood particles, but published literature is inconclusive about which structure change is the major reason for enhanced durability. In this work, steam treatment was combined either with alkaline or with SO2 to study. The solids obtained after steam treatments along with a control sample were dried and each was compacted into pellets. The pellets were then tested for durability. Steam treatment alone dominated improvements in durability. The pellet durability increased with the amount of xylose, but xylose performed better in the pellet from raw poplar than did in the pellet from treated poplar. Water-soluble components contributed a maximum 4% of the durability of poplar pellets. The addition of lignin and sugars to substrates after steam treatment did not improve durability significantly. The surface modification that took place as a result of size reduction during steam treatment was the major reason, contributing about 50% of the durability of the pellet from steam-treated poplar. The acidity of steam treatment slightly affected the relative contributions of these structure changes on the durability. The new knowledge helps tailor the chemical and/or mechanical pretreatment involved in pelleting biomass to durable pellets. 相似文献
187.
The individual, concomitant and sequential inoculation of second stage juveniles (at 2000 J2/kg soil) of Meloidogyne incognita and Rhizoctonia solani (at 2 g mycelial mat/kg soil) showed significant reduction in plant growth parameters viz. plant length, fresh weight and dry weight as compared to control. The greatest reduction in plant growth parameters was recorded in the plants simultaneously inoculated with M. incognita and R. solani followed by sequential and individual inoculation. In sequential inoculation, plant inoculated with M. incognita 15 days prior to R. solani shows more reduction in comparison to plant inoculated with R. solani 15 days prior to M. incognita. Moreover, the multiplication of nematode and number of galls/root system were significantly reduced in concomitant and sequential inoculation as compared to individual inoculation, whereas the intensity of root-rot/root system caused by R. solani was increased in the presence of root-knot nematode M. incognita as compared to when R. solani was inoculated individually. 相似文献
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189.
This paper evaluates the degree of saliency of texts in natural scenes using visual saliency models. A large scale scene image database with pixel level ground truth is created for this purpose. Using this scene image database and five state-of-the-art models, visual saliency maps that represent the degree of saliency of the objects are calculated. The receiver operating characteristic curve is employed in order to evaluate the saliency of scene texts, which is calculated by visual saliency models. A visualization of the distribution of scene texts and non-texts in the space constructed by three kinds of saliency maps, which are calculated using Itti''s visual saliency model with intensity, color and orientation features, is given. This visualization of distribution indicates that text characters are more salient than their non-text neighbors, and can be captured from the background. Therefore, scene texts can be extracted from the scene images. With this in mind, a new visual saliency architecture, named hierarchical visual saliency model, is proposed. Hierarchical visual saliency model is based on Itti''s model and consists of two stages. In the first stage, Itti''s model is used to calculate the saliency map, and Otsu''s global thresholding algorithm is applied to extract the salient region that we are interested in. In the second stage, Itti''s model is applied to the salient region to calculate the final saliency map. An experimental evaluation demonstrates that the proposed model outperforms Itti''s model in terms of captured scene texts. 相似文献
190.