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61.
Application of Maltodextrin as Chiral Selector in Capillary Electrophoresis for Quantification of Amlodipine Enantiomers in Commercial Tablets 下载免费PDF全文
Saeed Nojavan Shabnam Pourmoslemi Hamideh Behdad Ali Reza Fakhari Ali Mohammadi 《Chirality》2014,26(8):394-399
Maltodextrin was investigated as a chiral selector in capillary electrophoresis (CE) analysis of amlodipine (AM) enantiomers. For development of a stereoselective CE method, various effective parameters on the enantioseparation were optimized. The best results were achieved on an uncoated fused silica capillary at 20 °C using phosphate buffer (100 mM, pH 4) containing 10% w/v maltodextrin (dextrose equivalent value 4–7). The UV detector was set at 214 nm and a constant voltage of 20 kV was applied. The range of quantitation was 2.5–250 µg/mL (R2 > 0.999) for both enantiomers. Intra‐ (n = 5) and interday (n = 3) relative standard deviation (RSD) values were less than 7%. The limits of quantitation and detection were 1.7 µg/mL and 0.52 µg/mL, respectively. Recoveries of R(+) and S(?) enantiomers from tablet matrix were 97.2% and 97.8%, respectively. The method was applied for the quantification of AM enantiomers in commercial tablets. Also, the enantioseparation capability of heparin was evaluated and the results showed that heparin did not have any chiral selector activity in this study. Chirality 26:394–399, 2014. © 2014 Wiley Periodicals, Inc. 相似文献
62.
Suzuki K Noda J Yanagisawa M Kawazu I Sera K Fukui D Asakawa M Yokota H 《Biological trace element research》2012,146(3):302-308
The present study was designed to compare the effects of nano red selenium and sodium selenite on the antioxidative activities
of neutrophils and the hematological parameters in sheep. Fifteen sheep were randomly allocated into three groups. Groups
1 and 2 received selenium nanoparticles orally at 1 mg/kg and sodium selenite at 1 mg Se/kg for 10 consecutive days; group
3 served as the control. To assess the degrees of oxidative stress and of lipid peroxidation of the cellular membranes, the
levels of thiobarbituric acid reactive substances (TBARS) were determined in serum samples that were collected at different
supplementation intervals, i.e., after 0, 10, 20, and 30 days. In addition, hematological parameters in the serum samples
were measured by routine procedures. It was found that TBARS levels in groups 1 and 2 were significantly higher on days 20
and 30 compared to the basal level on day 0. It was also found that on day 30, the TBARS activities in both treated groups
were significantly higher than those of the controls (P < 0.05). These findings may explain the seemingly paradoxical effects of supplemental selenium on the indicators of oxidative
stress, as the levels of TBARS were generally expected to decrease in the presence of selenium. There were no significant
differences between the PCV and RBC values in the three groups. The white blood cell count (WBC) in group 1 showed a significant
increase on days 20 and 30 in comparison with the control group. However, in group 2, there was a significant increase of
the WBC value just on day 20 in comparison with the control group. Also, there were significant increases of the neutrophil
counts and significant decreases of the lymphocyte counts on day 10 in group 1, in comparison with those in group 2 and controls,
and on days 20 and 30 in groups 1 and 2 in comparison with those in the control group. 相似文献
63.
Shalapour S Deiser K Kühl AA Glauben R Krug SM Fischer A Sercan O Chappaz S Bereswill S Heimesaat MM Loddenkemper C Fromm M Finke D Hämmerling GJ Arnold B Siegmund B Schüler T 《PloS one》2012,7(2):e31939
Interleukin-7 (IL-7) is a major survival factor for mature T cells. Therefore, the degree of IL-7 availability determines the size of the peripheral T cell pool and regulates T cell homeostasis. Here we provide evidence that IL-7 also regulates the homeostasis of intestinal epithelial cells (IEC), colon function and the composition of the commensal microflora. In the colon of T cell-deficient, lymphopenic mice, IL-7-producing IEC accumulate. IEC hyperplasia can be blocked by IL-7-consuming T cells or the inactivation of the IL-7/IL-7R signaling pathway. However, the blockade of the IL-7/IL-7R signaling pathway renders T cell-deficient mice more sensitive to chemically-induced IEC damage and subsequent colitis. In summary, our data demonstrate that IL-7 promotes IEC hyperplasia under lymphopenic conditions. Under non-lymphopenic conditions, however, T cells consume IL-7 thereby limiting IEC expansion and survival. Hence, the degree of IL-7 availability regulates both, T cell and IEC homeostasis. 相似文献
64.
HLA-A*0201-restricted CD8+ cytotoxic T lymphocyte epitopes identified from herpes simplex virus glycoprotein D 总被引:1,自引:0,他引:1
Chentoufi AA Zhang X Lamberth K Dasgupta G Bettahi I Nguyen A Wu M Zhu X Mohebbi A Buus S Wechsler SL Nesburn AB BenMohamed L 《Journal of immunology (Baltimore, Md. : 1950)》2008,180(1):426-437
Evidence obtained from both animal models and humans suggests that T cells specific for HSV-1 and HSV-2 glycoprotein D (gD) contribute to protective immunity against herpes infection. However, knowledge of gD-specific human T cell responses is limited to CD4+ T cell epitopes, with no CD8+ T cell epitopes identified to date. In this study, we screened the HSV-1 gD amino acid sequence for HLA-A*0201-restricted epitopes using several predictive computational algorithms and identified 10 high probability CD8+ T cell epitopes. Synthetic peptides corresponding to four of these epitopes, each nine to 10 amino acids in length, exhibited high-affinity binding in vitro to purified human HLA-A*0201 molecules. Three of these four peptide epitopes, gD53-61, gD70-78, and gD278-286, significantly stabilized HLA-A*0201 molecules on T2 cell lines and are highly conserved among and between HSV-1 and HSV-2 strains. Consistent with this, in 33 sequentially studied HLA-A*0201-positive, HSV-1-seropositive, and/or HSV-2-seropositive healthy individuals, the most frequent and robust CD8+ T cell responses, assessed by IFN-gamma ELISPOT, CD107a/b cytotoxic degranulation, and tetramer assays, were directed mainly against gD53-61, gD70-78, and gD278-286 epitopes. In addition, CD8+ T cell lines generated by gD53-61, gD70-78, and gD278-286 peptides recognized infected target cells expressing native gD. Lastly, CD8+ T cell responses specific to gD53-61, gD70-78, and gD278-286 epitopes were induced in HLA-A*0201 transgenic mice following ocular or genital infection with either HSV-1 or HSV-2. The functional gD CD8+ T cell epitopes described herein are potentially important components of clinical immunotherapeutic and immunoprophylactic herpes vaccines. 相似文献
65.
Shabnam Mohammadi Mansoureh Movahedin Seyed Javad Mowla 《Reproductive biology and endocrinology : RB&E》2009,7(1):126-6
Background
CatSper1-4 are a unique family of sperm cation channels, which are exclusively expressed in the testis and play an important role in sperm motility and male fertility. Despite their vital role in male fertility, almost nothing is known about the factors regulating their expression. Here, we investigated the effects of selenium (Se) on the expression of CatSper genes and sperm parameters in aging versus young male mice. 相似文献66.
Distinct roles of the 7‐transmembrane receptor protein Rta3 in regulating the asymmetric distribution of phosphatidylcholine across the plasma membrane and biofilm formation in Candida albicans 下载免费PDF全文
67.
Sharif Moradi Parisa Torabi Saeed Mohebbi Sara Amjadian Piter Bosma Farnoush Faridbod Vahid Khoddami Morteza Hosseini Sadegh Babashah Maryam Ghotbaddini Arezoo Rasti Faezeh Shekari Hamid Sadeghi-Abandansari Jafar Kiani Mehdi Shamsara Mohammad Kazemi-Ashtiani Samira Gholami 《BioEssays : news and reviews in molecular, cellular and developmental biology》2020,42(6):2000042
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69.
Reza Zarezadeh Amir Mehdizadeh Jo L. M. R. Leroy Mohammad Nouri Shabnam Fayezi Masoud Darabi 《Journal of cellular physiology》2019,234(2):1016-1029
Infertility is a growing problem worldwide. Currently, in vitro fertilization (IVF) is widely performed to treat infertility. However, a high percentage of IVF cycles fails, due to the poor developmental potential of the retrieved oocyte to generate viable embryos. Fatty acid content of the follicular microenvironment can affect oocyte maturation and the subsequent developmental competence. Saturated and monounsaturated fatty acids are mainly used by follicle components as primary energy sources whereas polyunsaturated fatty acids (PUFAs) play a wide range of roles. A large body of evidence supports the beneficial effects of n-3 PUFAs in prevention, treatment, and amelioration of some pathophysiological conditions including heart diseases, cancer, diabetes, and psychological disorders. Nevertheless, current findings regarding the effects of n-3 PUFAs on reproductive outcomes in general and on oocyte quality more specifically are inconsistent. This review attempts to provide a comprehensive overview of potential molecular mechanisms by which n-3 PUFAs affect oocyte maturation and developmental competence, particularly in the setting of IVF and thereby aims to elucidate the reasons behind current discrepancies around this topic. 相似文献
70.
In this research, the effects of a solidification/stabilization (S/S) technique on the remediation of cresol-contaminated soil were investigated. The soil samples were collected from the Tehran Oil Refinery (TOR) in Ray District, Iran. Cresols are hazardous chemicals whose exposure at high dosage results in irreparable damage to animal and human health. S/S process progresses through physicochemical reactions, reducing the leachability of a waste as well as changing its physical properties in order to encapsulate the contaminants and form a solid material. The samples were spiked by certain concentrations of cresols including meta-, ortho-, and para-isomers. The treatment process was conducted using Portland cement as the binder and modified bentonite as a stabilizer. The efficiency of the S/S technique was assessed by the unconfined compressive strength (UCS) test and toxicity characteristic leaching procedure (TCLP) test. The results of the tests showed that adding 20% and 30% Portland cement in combination with 15% and 30% modified bentonite to contaminated samples increased the 28-day compressive strength of S/S blocks to a range of 2.44 to 3.08 MPa. In fact, increasing the proportion of cement in the mix design resulted in enhanced compressive strength, while modified bentonite inversely affected samples’ strength. Regarding leaching behavior, adding organophilic clay to polluted samples noticeably declined cresol's concentrations in leachate. Overall efficiency of S/S in terms of leaching was about 70%, which was obtained by adding 20% cement and 30% modified clay. A logarithmic relationship between leaching percent and modified clay ratio was also detected with high logarithmic and linear correlation coefficients of 0.96 and 0.9, respectively, hence presenting the efficiency of S/S in stabilizing the cresols in the samples. 相似文献