全文获取类型
收费全文 | 1629篇 |
免费 | 20篇 |
国内免费 | 60篇 |
出版年
2021年 | 23篇 |
2020年 | 18篇 |
2019年 | 29篇 |
2018年 | 34篇 |
2017年 | 24篇 |
2016年 | 29篇 |
2015年 | 18篇 |
2014年 | 27篇 |
2013年 | 76篇 |
2012年 | 32篇 |
2011年 | 60篇 |
2010年 | 50篇 |
2009年 | 33篇 |
2008年 | 51篇 |
2007年 | 36篇 |
2006年 | 31篇 |
2005年 | 38篇 |
2004年 | 41篇 |
2003年 | 30篇 |
2002年 | 41篇 |
2001年 | 23篇 |
2000年 | 38篇 |
1999年 | 16篇 |
1992年 | 39篇 |
1991年 | 49篇 |
1990年 | 44篇 |
1989年 | 50篇 |
1988年 | 54篇 |
1987年 | 49篇 |
1986年 | 40篇 |
1985年 | 46篇 |
1984年 | 37篇 |
1983年 | 24篇 |
1982年 | 17篇 |
1981年 | 18篇 |
1980年 | 17篇 |
1979年 | 23篇 |
1978年 | 24篇 |
1977年 | 21篇 |
1976年 | 25篇 |
1975年 | 23篇 |
1974年 | 30篇 |
1973年 | 26篇 |
1972年 | 32篇 |
1971年 | 19篇 |
1970年 | 19篇 |
1969年 | 20篇 |
1968年 | 21篇 |
1967年 | 17篇 |
1966年 | 24篇 |
排序方式: 共有1709条查询结果,搜索用时 31 毫秒
101.
Molecular Biology - The protein synthesis in cells occurs in ribosomes, with the involvement of protein translational factors. One of these translational factors is the elongation factor P (EF-P).... 相似文献
102.
Belyanko T. I. Feoktistova E. S. Skrypina N. A. Skamrov A. V. Gurskii Ya. G. Rutkevich N. M. Dobrynina N. I. Bibilashvilli R. Sh. Savochkina L. P. 《Biophysics》2019,64(3):331-338
Biophysics - Abstract—Variants of miniplasminogen with an altered primary structure have been designed to study previously described changes in tryptophan fluorescence during plasminogen... 相似文献
103.
104.
105.
106.
107.
108.
Alekseenkova VA Belianko TI Timofeeva AV Savochkina LP Heumann H Bibilashvili RSh 《Molekuliarnaia biologiia》2001,35(5):879-899
The kinetic characteristics have been studied for noncircularly permuted variants of the human hepatitis delta virus (HDV) antigenomic ribozyme to find out the cause of the two-phase kinetics of the self-cleavage reaction. Different ways of reaction initiation, suboptimal conditions, and jumpwise changes of reaction conditions have been used, and the temperature dependences have been studied. A correlation has been shown between the apparent kinetic constant of the first reaction phase and the portion of the ribozyme molecules that self-cleaved during the first phase. Partial restoration of the initial reaction characteristics has been shown by the reinitiation of reaction being stopped after completing the first phase. On the basis of all the data obtained, a scheme of the self-cleavage reaction has been proposed including: (i) activation of the ribozyme with energy of 40-50 kcal/mol and a characteristic time of several deciminutes under optimal reaction conditions; (ii) fast and reversible reaction of the phosphodiester bond cleavage; (iii) reaction leading to isomerization of the 3',5'-phosphodiester bond to the 2',5' bond in the self-cleavage site with a characteristic activation time of tens of minutes; and (iv) practically irreversible conformational change leading to fixation of the cleavage by immobilization of the 5'-terminal nucleotide of the product in the center of the formed structure and displacement of the 3'-terminal nucleotide to the periphery. The latter process has a characteristic time of tens of minutes and a low activation energy. 相似文献
109.
Tumanova OIu Kuvshinov VN Azaev MSh Masharskiĭ AE Klimov NA Kozlov AP Il'ichev AA Sandakhchiev LS 《Molekuliarnaia biologiia》2001,35(1):146-151
A phase peptide library was screened with virus-neutralizing monoclonal antibodies (MCA) 2F5 which recognize a conserved epitope of HIV-1 gp41. Phages that expose peptides specifically binding with MCA 2F5 were selected by ELISA. Amino acid sequence analysis revealed a homology to region 662-671 of HIV-1 HB10 gp160 for most peptides. The major role in recognition was ascribed to Asp-664, Lys-665, and Trp-666. The epitope-mimicking peptides were tested for immunogenicity. Antibodies to gp41 were detected in serum of immunized rabbits. 相似文献
110.