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281.
We cloned a gene, kexD, that provides a multidrug-resistant phenotype from multidrug-resistant Klebsiella pneumoniae MGH78578. The deduced amino acid sequence of KexD is similar to that of the inner membrane protein, RND-type multidrug efflux pump. Introduction of the kexD gene into Escherichia coli KAM32 resulted in a MIC that was higher for erythromycin, novobiocin, rhodamine 6G, tetraphenylphosphonium chloride, and ethidium bromide than that of the control. Intracellular ethidium bromide levels in E. coli cells carrying the kexD gene were lower than that in the control cells under energized conditions, suggesting that KexD is a component of an energy-dependent efflux pump. RND-type pumps typically consist of three components: an inner membrane protein, a periplasmic protein, and an outer membrane protein. We discovered that KexD functions with a periplasmic protein, AcrA, from E. coli and K. pneumoniae, but not with the periplasmic proteins KexA and KexG from K. pneumoniae. KexD was able to utilize either TolC of E. coli or KocC of K. pneumoniae as an outer membrane component. kexD mRNA was not detected in K. pneumoniae MGH78578 or ATCC10031. We isolated erythromycin-resistant mutants from K. pneumoniae ATCC10031, and some showed a multidrug-resistant phenotype similar to the drug resistance pattern of KexD. Two strains of multidrug-resistant mutants were investigated for kexD expression; kexD mRNA levels were increased in these strains. We conclude that changing kexD expression can contribute to the occurrence of multidrug-resistant K. pneumoniae. 相似文献
282.
Mousavi SR Khalaji I Sadeghi Naini A Raahemifar K Samani A 《Computer methods in biomechanics and biomedical engineering》2012,15(6):595-608
The finite element (FE) method can accurately calculate tissue deformation. However, its low speed renders it ineffective for many biomedical applications involving real-time data processing. To accelerate FE analysis, we introduce a novel tissue mechanics simulation technique. This technique is suitable for real-time estimation of tissue deformation of specific organs, which is required in computer-aided diagnostic or therapeutic procedures. In this method, principal component analysis is used to describe each organ shape and its corresponding FE field for a pool of patients by a small number of weight factors. A mapping function is developed to relate the parameters of organ shape to their FE field counterpart. We show that irrespective of the complexity of the tissue's constitutive law or its loading conditions, the proposed technique is highly accurate and fast in estimating the FE field. Average deformation errors of less than 2% demonstrate the accuracy of the proposed technique. 相似文献
283.
284.
Gharibzahedi SM Mousavi SM Khodaiyan F Hamedi M 《International journal of biological macromolecules》2012,50(2):376-384
A central composite rotatable design (CCRD) was used to evaluate the effects of walnut oil (WO, 3-6%, w/w) and gum arabic (GA, 5-10%, w/w) on the average droplet size (D(32)), specific surface area (SSA), polydispersity index (span), apparent viscosity, interfacial tension and opacity of walnut-beverage emulsions. The response surface methodology (RSM) showed that the significant second-order polynomial regression equations with high R(2) (>0.95) were successfully fitted for all responses as function of independent variables. The linear effect of WO had a significant term in all reduced models. The overall optimum region was found to be at the combined level of 10% (w/w) GA content and 5.84% (w/w) WO concentration. At this optimum point, D(32), SSA, span, apparent viscosity, interfacial tension and opacity of emulsions were 0.609 μm, 8.236 m(2)/ml, 0.886, 1.336 Pa s, 51.37 mN/m and 0.810, respectively. No significant (p>0.05) difference was found between the actual values and predicted values. Moreover, principal component analysis (PCA), conducted via PCA variable loadings and cluster dendrogram was able to discriminate the emulsions with different formulations into separate classes. 相似文献
285.
Anaerobic oxidation of volatile fatty acids (VFAs) as the key intermediates is restricted thermodynamically. Presently, enriched
acetogenic and methanogenic cultures were used for syntrophic anaerobic digestion of VFAs in an upflow anaerobic sludge bed
reactor fed with acetic, propionic, and butyric acids at maximum concentrations of 5.0, 3.0, and 4.0 g/L, respectively. Interactive
effects of propionate, butyrate and acetate were analyzed. Hydraulic retention time (HRT) and acetate oxidizing syntrophs
and methanogen (hydrogenotrophs) to syntrophic bacteria (propionate- and butyrate-oxidizing bacteria) population ratio (M/A)
were investigated as key microbiological and operating variables of VFA anaerobic degradations. M/A did not affect the size
distribution and had little effect on extracellular polymer contents of the granules. Granular sludge with close spatial microbial
proximity enhanced syntrophic degradation of VFAs compared to other cultures, such as suspended cultures. Optimum conditions
were found to be propionate = 1.93 g/L, butyrate = 2.15 g/L, acetate = 2.50 g/L, HRT = 22 h, and M/A = 2.5 corresponding to
maximum VFA removal and biogas production rate. Results of verification experiments and predicted values from fitted correlations
were in close agreement at the 95% confidence interval. Granules seemed to be smaller particles and less stable in construction
with an irregular fractured surface compared to the original granules. 相似文献
286.
287.
Length–weight relationships and condition factors of two fish species from the southern Caspian Sea basin: Alburnoides samiii Mousavi‐Sabet,Vatandoust & Doadrio, 2015 and Ponticola iranicus Vasil′eva,Mousavi‐Sabet & Vasil′ev, 2015
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H. Mousavi‐Sabet A. Heidari M. Mohammadi‐Darestani M. Mansouri‐Chorehi K. Ghasemzadeh 《Zeitschrift fur angewandte Ichthyologie》2016,32(4):751-752
The present study describes the length–weight relationships (LWRs) and condition factors (K) of Alburnoides samiii and Ponticola iranicus in the Sefidroud River from the southern Caspian Sea basin, September 2013 to August 2014. This study presents the first known reference in the FishBase database regarding the LWRs and condition factors for these two indigenous species. 相似文献
288.
M. A. Mohammad Mirzaie S. M. Mousavi B. Ghobadian 《Preparative biochemistry & biotechnology》2016,46(2):150-156
Growth of Chlorella vulgaris and its lipid production were investigated under autotrophic, heterotrophic, and mixotrophic conditions. Cheap agricultural waste molasses and corn steep liquor from industries were used as carbon and nitrogen sources, respectively. Chlorella vulgaris grew remarkably under this agricultural waste medium, which resulted in a reduction in the final cost of the biodiesel production. Maximum dry weight of 2.62 g L?1 was obtained in mixotrophic growth with the highest lipid concentration of 0.86 g L?1. These biomass and lipid concentrations were, respectively, 140% and 170% higher than autotrophic growth and 300% and 1200% higher than heterotrophic growth. In mixotrophic growth, independent or simultaneous occurrence of autotrophic and heterotrophic metabolisms was investigated. The growth of the microalgae was observed to take place first heterotrophically to a minimum substrate concentration with a little fraction in growth under autotrophic metabolism, and then the cells grew more autotrophically. It was found that mixotrophic growth was not a simple combination of heterotrophic and autotrophic growth. 相似文献
289.
Khodaiyan F Razavi SH Emam-Djomeh Z Mousavi SM Hejazi MA 《Journal of microbiology and biotechnology》2007,17(2):195-201
This study investigated the effects of various culture parameters (carbon sources, temperature, initial pH of culture, NaCl concentration, and light) on the growth and canthaxanthin production by Dietzia natronolimnaea HS-1. The results showed that the most effective carbon source for growth and canthaxantin production was glucose, and the best pH and temperature were 7 and 31 degrees C, respectively. In addition, the biomass and canthaxanthin production increased in a medium without NaCl and in the presence of light. Under the optimized conditions, the maximum biomass, total carotenoid, and canthaxanthin production were 6.12 +/- 0.21 g/l, 4.51 +/- 0.20 mg/l, and 4.28 +/- 0.15 mg/l, respectively, in an Erlenmeyer flask system, yet increased to 7.25 g/l, 5.48 mg/l, and 5.29 mg/l, respectively, in a batch fermenter system. 相似文献
290.
Ashkan Madadlou Daniela Iacopino David Sheehan Zahra Emam-Djomeh Mohammad E. Mousavi 《Biochimica et Biophysica Acta (BBA)/General Subjects》2010