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11.
Sepideh Zununi Vahed Haleh Forouhandeh Salar Hassanzadeh Hans-Peter Klenk Mohammad Amin Hejazi Mohammad Saeid Hejazi 《Microbiology》2011,80(6):834-841
Urmia Lake is one of the most permanent hypersaline lakes in the world which is threatened by hypersalinity and serious dryness.
In spite of its importance no paper has been published regarding bacterial community of this lake. Accordingly, the present
study aimed to investigate the halophilic bacteria in the aforementioned lake. In so doing, thirty seven strains were isolated
on six different culture media. The isolated strains were characterized using phenotypic and genotypic methods. Growth of
the strains occurred at 25–35°C, pH 6–9 and 7 to 20% (w/v) NaCl indicating that most of the isolates were moderately halophiles.
Catalase, oxidase and urease activities were found to be positive for the majority of the isolates. Phylogenetic analysis
based on 16S rRNA gene sequences indicated that the isolated bacteria belonged to two major taxa: Gammaproteobacteria (92%, including Salicola [46%], Pseudomonas [13.5%], Marinobacter [11%], Idiomarina [11%], and Halomonas [8%]) and Firmicutes (8%, including Bacillus [5%] and Halobacillus [3%]). In addition, a novel bacterium whose 16S rRNA gene sequence showed almost 98% sequence identity with the taxonomically
troubled DSM 3050T, Halovibrio denitrificans HGD 3T and Halospina denitriflcans HGD 1–3, each, was isolated. 16S rRNA gene similarity levels along with phenotypic characteristics suggest that some of the
isolated strains could be regarded as potential type strain for novel species, on which further studies are recommended. 相似文献
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Behzad Dehghani Iraj Rasooli Seyed Latif Mousavi Gargari Mohammad Reza Jalali Nadooshan Parviz Owlia Shahram Nazarian 《Microbiological research》2013,168(2):84-90
Acellular vaccines containing bacterial immunodominant components such as surface proteins may be potent alternatives to live attenuated vaccines in order to reduce salmonellosis risk to human health. invH gene, an important part of needle complex in type three secretion system (TTSS) plays important role in efficient bacterial adherence and entry into epithelial cells. In this work we hypothesize that use of a 15 kDa recombinant InvH as Salmonella enterica serovar Enteritidis surface protein could provoke antibody production in mouse and would help us study feasibility of its potential for diagnosis and/or a recombinant vaccine. The purified InvH provoked significant rise of IgG in mice. Active protection induced by immunization with InvH against variable doses of S. enterica serovar Enteritidis, indicated that the immunized mice were completely protected against challenge with 104 LD50. The immunoreaction of sera from immunized mice with other Salmonella strains or cross reaction with sera of Salmonella strains inoculated mice is indicative of possessing by Salmonella strains of the surface protein, InvH, that can be employed in both prophylactic and diagnostic measures against S. enterica. Bacteria free spleen and ileum of the immunized mice in this study indicate that the invH gene affects bacterial invasion. Efficacy of the virulence protein, InvH, in shuttling into host cells in injectisome of S. enterica serovar Enteritidis and inhibition of this phenomenon by active immunization was shown in this study. In conclusion immunization with InvH protein can develop protection against S. enterica serovar Enteritidis infections. InvH in Salmonella strains can be exploited in protective measures as well as a diagnostic tool in Salmonella infections. 相似文献
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Gholamabbas Sayyad Majid Afyuni Sayed-Farhad Mousavi Karim C. Abbaspour Mohammad A. Hajabbasi Brian K. Richards 《Soil & Sediment Contamination》2009,18(2):216-228
Accumulation of heavy metals (HMs) in cultivated soils is a continuing environmental problem in many parts of the world. An increase in HM concentration can enhance uptake of toxic metals by crops and enter the human food chain. In this study, the uptake behavior of wheat and safflower was evaluated in a calcareous soil by using 12 undisturbed columns in which half were artificially contaminated. Heavy metals in the form of CdCl2 (15 mg Cd kg? 1), CuSO4 (585 mg Cu kg? 1), Pb(NO3)2 (117 mg Pb kg? 1), and ZnCl2 (1094 mg Zn kg? 1) were sprayed on the soil surface and completely mixed in the top 10 cm. The background total concentrations of Cd, Cu, Pb and Zn were 1.6, 29.5, 17.5 and 61.2 mg kg? 1, respectively. After metal application, half of the columns (3 contaminated and 3 uncontaminated) were sown with wheat (Triticum aestivum) and the other half with safflower (Carthamus tinctorious) and grown for 74 days until maturity. After harvesting, soil columns were cut into 10-cm sections and analyzed for HNO3- and DTPA-extractable metal concentrations. Metal concentrations were also measured in different plant tissues. The results showed that artificial contamination of topsoil decreased the transpiration rate of wheat by 12% and that of safflower by 6%. In contaminated columns, Cd, Cu, Pb, and Zn accumulation in wheat shoot was greater by 8.0-, 1.9-, 3.0-, and 2.1-fold than the control, respectively. Accordingly, these numbers were 46.0-, 1.3-, 1.7-, and 1.6-fold in safflower shoot. Soil contamination with HMs resulted in a 55% decrease in shoot dry matter yield of wheat while it had no significant effect on shoot dry matter of safflower. The normalized water consumption for safflower was therefore not affected by metal contamination (≈ 13 mm H2O g? 1 of dry weight for all safflower and uncontaminated wheat treatments), while contaminated wheat was much less water efficient at about 27 mm H2O g? 1 dry weight. It was concluded that although artificial contamination had a negative effect on wheat growth, it did not affect safflower's normal growth and water efficiency. 相似文献
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Bovine serum albumin (BSA) has various applications in blood group serology and different research purposes. In this study purification of BSA has been compared with human serum albumin (HSA) using modified ethanol precipitation method based on the method of Cohn. The purification process was carried out under controlled conditions, particularly of ethanol concentration, pH, ionic strength and temperature. It was revealed that the produced BSA and HSA have purity more than 95%. It is obvious that HSA can be used, as a drug when the amount of its polymers is less than 5% whereas polymer generation is required in order to enhance the potentiating properties of BSA in agglutination of red cells. We propose here a simple and rapid two-step method for simultaneously purification and polymerization of BSA. By this method simply BSA with desired amount of polymers was obtained by 40% ethanol concentration. 相似文献
18.
Length–weight and length–length relationships for two gobiids from the Anzali Wetland,in the southern Caspian Sea basin 下载免费PDF全文
A. Heidari H. Mousavi‐Sabet M. Sattari M. S. Alavi‐Yeganeh K. Abbasi 《Zeitschrift fur angewandte Ichthyologie》2018,34(4):1042-1044
Length–weight (LWR) and length–length (LLR) relationships were estimated for specimens of two gobiid species collected from the Anzali Wetland and its related streams in the southern Caspian Sea basin, in August 2017. These represent the first reports of LWR and LLR data for Rhinogobius cf. similis Gill, 1859 (37°28′13″N, 49°20′33″E; 50 individuals) and Proterorhinus nasalis (De Filippi, 1863) (36°54′10.89″N, 53°48′48.33″E; 30 individuals) from the Wetland. A new maximum length is reported for P. nasalis. The length–weight parameter b for these species ranged a minimum of 2.99 for Rhinogobius cf. similis to a maximum of 3.04 for P. nasalis with regression coefficients (r2) ranging from .95 to .97. All LLRs were highly significant (r2>.96). 相似文献
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Length‐weight relationships for five Clupeiformes species from the Persian Gulf and Oman Sea 下载免费PDF全文
H. Mousavi‐Sabet H. Homayouni M. Marjani A. Heidari 《Zeitschrift fur angewandte Ichthyologie》2016,32(1):169-170
Length‐weight relationships (LWRs) were determined for five Clupeiformes species representing three families collected from the Persian Gulf and Oman Sea. These are the first references on length‐weight relationships for two of these species (Ilisha megaloptera and Sardinella sindensis). A new maximum length record was obtained for one species (Ilisha melastoma). 相似文献