全文获取类型
收费全文 | 1294篇 |
免费 | 123篇 |
国内免费 | 1篇 |
出版年
2024年 | 2篇 |
2023年 | 16篇 |
2022年 | 49篇 |
2021年 | 88篇 |
2020年 | 76篇 |
2019年 | 145篇 |
2018年 | 123篇 |
2017年 | 83篇 |
2016年 | 63篇 |
2015年 | 67篇 |
2014年 | 75篇 |
2013年 | 98篇 |
2012年 | 103篇 |
2011年 | 89篇 |
2010年 | 49篇 |
2009年 | 47篇 |
2008年 | 46篇 |
2007年 | 43篇 |
2006年 | 24篇 |
2005年 | 19篇 |
2004年 | 22篇 |
2003年 | 13篇 |
2002年 | 14篇 |
2001年 | 8篇 |
2000年 | 4篇 |
1999年 | 5篇 |
1998年 | 8篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1994年 | 4篇 |
1993年 | 7篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1977年 | 1篇 |
1968年 | 1篇 |
1920年 | 1篇 |
1911年 | 1篇 |
排序方式: 共有1418条查询结果,搜索用时 15 毫秒
951.
Homeira Rashidi Hajieh Shahbazian Forogh Nokhostin Seyed Mahmood Latifi Mehrian Jafarizade 《生物学前沿》2018,13(6):452-457
Background and Aim
The prevalence of metabolic syndrome (MS) increased in recent years in both adolescents and children groups. The aim of the study is evaluating the relationship between insulin and uric acid (UA) level in MS in adolescentsMaterials and Methods
we studied 120 adolescence aged 10 to 19 in two groups: control group without metabolic syndrome and case group with metabolic syndrome. The Criteria of ATP III was considered as a diagnosis factor for metabolic syndrome.Discussion
Various studies have been conducted in various populations to evaluate the relationship between UA level and MS in adolescents. Abdominal obesity, low HDL, hypertriglyceridemia and hypertension are associated with high UA level. In their analysis, the MS OR in UA level?4.9, 4.9-5.8 and ?5.8 mg/dl was 1, 2.53 and 9.03, respectively, which were higher than our findings in current study. Hyperinsulinemia caused by insulin resistance is one of the complications associated with MS, which puts individuals at risk of diabetes and cardiovascular events.Results
Uric acid level in the Case group was significantly higher than the control group (p = 0.0001, 43.8±1.4 vs. 4.1±1 mg/dl, respectively). Insulin level was significantly higher in the case group in compare to the control group (p = 0.008, 9.8± 5.3 vs. 12.2±6 μU/ml, respectively).Conclusion
The findings of this case-control study showed that adolescents with metabolic syndrome have a higher uric acid and insulin level in compare to normal subjects. We hypothesis that increase in serum insulin and uric acid level can be a risk factor in the development of metabolic syndrome.952.
Mahmoud Hashemzaei Amir Hooshang Mohammadpour Mohsen Imenshahidi Ramin Rezaee Seyed Adel Moallem 《Biologia》2018,73(11):1153-1157
Carbon monoxide (CO) produced by incomplete combustion of hydrocarbons, has many toxic effects on different organs, especially the heart and brain that have greater demands for oxygen. The present study aimed to evaluate the protective effects of granulocyte colony stimulating factor (G-CSF) on apoptosis after CO poisoning in rats. Male Wistar rats were exposed to CO 1500 or 3000 ppm for 60 min. Single and multiple doses of G-CSF (10, 50, and 100 μg/kg) were administered to animals. After CO poisoning, carboxyhemoglobin concentration was measured, apoptotic cells were evaluated by TUNEL assay and caspase 3 activity was determined by immunofluorescence. Blood levels of carboxyhemoglobin significantly increased following exposure to both 1500 and 3000 ppm concentrations of CO. However, carboxyhemoglobin levels were significantly higher following exposure to CO 3000 ppm compared to CO 1500 ppm (p?<?0.05). Differences in caspase 3 activity between G-CSF and control groups were significant and G-CSF could decrease apoptosis following CO 3000 ppm poisoning (p?<?0.001). TUNEL assay showed that in rats treat with 5 doses of G-CSF 100 μg/kg, apoptosis was significantly ameliorated compared to control rats and sham (rats that were not exposed to CO) group (p?<?0.05). Concerning caspase 3 activity and apoptosis rate, the best results were found in rats exposed to 3000 ppm and treated with G-CSF 100 μg/kg. In this study, we confirmed that CO poisoning leads to cardiomyocytes apoptosis which could be significantly reduced by G-CSF treatment. 相似文献
953.
954.
955.
Tadayon Mohammad Saeed Hosseini Seyed Mashaallah 《Journal of Plant Growth Regulation》2023,42(2):735-747
Journal of Plant Growth Regulation - Among the different olive cultivars, ‘Shengeh’ olive trees have a poor yield, due to the incomplete flower bud development and increment of... 相似文献
956.
Pooya Fattahi Najmeh Salehi Zahra Azizi Javad Mohammadi Amir Norouzy Seyed Mohammad Moazzeni 《Journal of peptide science》2023,29(9):e3480
One of the most successful strategies in designing peptide-based cancer vaccines is modifying natural epitope peptides to increase their binding strength to human leukocyte antigens (HLAs). Anchor-modified Mart-1 peptide (ELAGIGILTV) is among the artificial epitope peptides with the highest binding affinity for HLA-A*0201. In this study, by fluorescence labeling of its either C- or N-terminus with Nε-(5-carboxyfluorescein)-l -lysine, we not only made it traceable but also drastically increased its binding strength to HLA-A*0201. HLA streptamer, for the first time, is introduced for measuring the binding constants (Ka) of the labeled peptides. The affinity of the labeled peptides for the HLA-A*201 of the MCF-7 cells was extraordinarily high and co-incubating them with the highest possible amount of the unlabeled peptide, as a competitor, did not significantly prohibit them from binding to the HLA. The reproducibility of the obtained results was confirmed by using the T2 cell line. The HLA-deficient K562 cell line was used as the negative control. With in silico simulations, we found two hydrophobic pockets on both sides of HLA-A*0201 for anchoring the C- or N-terminal 5-carboxyfluorescein probe, which can explain the extraordinary affinity of the labeled peptides for the HLA-A*0201. 相似文献
957.
Octavio L. Guterres Fernandes Tiago Tizziani Bibiana P. Dambrós Natália Ferreira de Sousa Carime L. Mansur Pontes Layzon A. L. da Silva Luiz A. Escorteganha Pollo Francisco F. de Assis Marcus T. Scotti Luciana Scotti Antonio L. Braga Mario Steindel Louis P. Sandjo 《化学与生物多样性》2023,20(3):e202201151
SARS-CoV-2 main protease (Mpro) plays an essential role in proteolysis cleavage that promotes coronavirus replication. Thus, attenuating the activity of this enzyme represents a strategy to develop antiviral agents. We report inhibitory effects against Mpro of 40 synthetic chalcones, and cytotoxicity activities, hemolysis, and in silico interactions of active compounds. Seven of them bearing a (E)-3-(furan-2-yl)-1-arylprop-2-en-1-one skeleton ( 10 , 28 , and 35–39 ) showed enzyme inhibition with IC50 ranging from 13.76 and 36.13 μM. Except for 35 and 36 , other active compounds were not cytotoxic up to 150 μM against THP-1 and Vero cell lines. Compounds 10 , and 35–39 showed no hemolysis while 28 was weakly hemotoxic at 150 μM. Moreover, molecular docking showed interactions between compound 10 and Mpro (PDBID 5RG2 and 5RG3) with proximity to cys145 and His41, suggesting a covalent binding. Products of the reaction between chalcones and cyclohexanethiol indicated that this binding could be a Michael addition type. 相似文献
958.
Yousefsani Seyed Abdolmajid Karimi Mohammad Zohoor Vahid 《Biomechanics and modeling in mechanobiology》2023,22(2):495-513
Biomechanics and Modeling in Mechanobiology - Biomechanical study of brain injuries originated from mechanical damages to white matter tissue requires detailed information on mechanical... 相似文献
959.
Summary Genomic DNA from 18 phylogenetically diverse species of bony fish was hybridized with probes specific for the channel catfish
immunoglobulin heavy chain constant (CH) gene, as well as with immunoglobulin heavy chain variable (VH) probes specific for
five channel catfish VH gene families. The results showed that CH probes strongly hybridized only to genomic fragments from
other catfish species. In contrast, restricted DNA from most other species hybridized with at least two channel catfish VH
probes. In those species whose DNA hybridized with multiple VH probes, the restriction pattern of hybridizing fragments was
probe-dependent. These studies suggest that (1) the CH gene defined in channel catfish appears to share similarity only with
CH genes in other catfish species, (2) families of VH genes appear to have diverged in early phylogenetic lineages of teleosts,
and (3) VH genes similar to those defined in catfish appear to be widely represented in phylogenetically diverse species of
teleosts. 相似文献
960.