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981.
Saeedeh Rastakhiz Mona Yazdani Sheida Shariat Atefeh Arab Amir Abbas Momtazi-Borojeni Nastaran Barati Mercedeh Mansourian Mohamdreza Amin Azam Abbasi Zahra Saberi Seyed Amir Jalali Ali Badiee Mahmoud Reza Jaafari 《Journal of cellular biochemistry》2019,120(2):1294-1303
The study was aimed at evaluating antitumor and immunomodulatory effects of liposomal vaccine composed of P5 human epidermal growth factor receptor 2 (HER2)/neu-derived peptide coupled to the surface of high-temperature nanoliposomes containing distearoylphosphocholine:distearoylphosphoglycerol:Chol:dioleoylphosphatidylethanolamine (DOPE) comprising monophosphoryl lipid A (MPL) adjuvant in HER2/neu overexpressing the breast cancer model. BALB/c mice bearing TUBO carcinoma were subcutaneously immunized with formulations containing 10 µg P5 peptide and 25 µg MPL three times with 2-week intervals. To determine immuno responses in immunized mice, the amount of released interferon-γ and IL-4 were measured by the enzyme-linked immunospot method and the flow cytometric analysis on the isolated splenocytes. The results demonstrated that tumor-bearing mice immunized with Lip/DOPE/MPL/P5 formulation had the most released interferon-γ and the highest cytotoxic T lymphocyte responses that led to the lowest tumor size and the longest survival time than those of other formulations. The results achieved by Lip/DOPE/MPL/P5 formulation could make it a suitable candidate to induce effective antigen-specific tumor immunity against breast cancer. 相似文献
982.
Simin Asadian Mahdi Alibabrdel Nazanin Daei Hadi Cheraghi Seyedeh Maedeh Jafari Elnaz Noshadirad Masoome Jabarpour Vahid Siavashi Seyed Mahdi Nassiri 《Journal of cellular biochemistry》2019,120(5):7115-7124
Type 2 diabetes (T2DM) is associated with an increased vascular disease. Moreover, endothelial progenitor cell (EPC) function is impaired in diabetic patients. Decreased EPC number plays a critical role in reduced endothelial repair and development of the vascular disorder. To determine the effect of metformin and insulin plus metformin on functional activity of EPCs, 130 participants were divided into three groups (group 1: healthy control; group 2: metformin; group 3: insulin plus metformin). The concentration of EPCs in the circulation was first quantified. Thereafter, circulating EPCs (cEPCs) were harvested and the biological features of these cells including proliferative, clonogenicity, tubulogenic, and migratory properties were analyzed after expansion. The serum protein levels of some proangiogenic factors were also measured. Our results showed greater numbers of cEPCs in control and in diabetic patients treated with insulin plus metformin than in metformin-treated patients. Insulin plus metformin therapy was associated with augmented proliferative, clonogenicity, migratory, and tubulogenic activity of cEPCs in patients with T2DM. Increased serum concentrations of angiogenic factors were also observed in patients treated with insulin plus metformin. Western blot analysis showed increased protein levels of pTie-2/Tie2 and Pakt/AKT in cEPCs harvested from T2DM, treated with insulin metformin plus. This study showed that treatment with insulin plus metformin in diabetic patients is associated with increased mobilization of EPCs into the circulation, with potential beneficial effect in vascular protection in diabetic patients. 相似文献
983.
984.
Elizabeth K. Chang Mark A. Eckert M. Monsur Ali Hamidreza Riazifar Egest J. Pone Linan Liu Weian Zhao 《PloS one》2015,10(3)
Multivalent interactions occur frequently in nature, where they mediate high-affinity interactions between cells, proteins, or molecules. Here, we report on a method to generate multivalent aptamers (Multi-Aptamers) that target L-selectin function using rolling circle amplification (RCA). We find that the L-selectin Multi-Aptamers have increased affinity compared to the monovalent aptamer, are specific to L-selectin, and are capable of inhibiting interactions with endogenous ligands. In addition, the Multi-Aptamers efficiently inhibit L-selectin mediated dynamic adhesion in vitro and homing to secondary lymphoid tissues in vivo. Importantly, our method of generating multivalent materials using RCA avoids many of the challenges associated with current multivalent materials in that the Multi-Aptamers are high affinity, easily produced and modified, and biocompatible. We anticipate that the Multi-Aptamers can serve as a platform technology to modulate diverse cellular processes. 相似文献
985.
Zahra Alimanesh Zeynab Alimanesh Fatemeh Davari Saeedeh Shadmehri Mozhgan Ahmadi Seyed Ali Hosseini Sepideh Dolati Alemeh Hariri Far 《Molecular & cellular biomechanics : MCB》2020,17(4):155-163
Diabetes mellitus (DM) disease can affect process of apoptosis by
increasing oxidative stress, nevertheless exercise and crocin can improve apoptosis; therefore present study aimed to investigate the effect of continued training
with crocin on apoptosis markers in liver tissue of diabetic rats. In this experimental study 32 diabetic rats based on fasting glucose divided into four groups of
eight rats including: 1) sham, 2) training, 3) crocin, and 4) training with crocin
also for investigate the effect of DM induction on apoptosis markers, eight healthy
rats assigned in healthy control group. During eight weeks groups 2 and 4 ran
60 minutes on treadmill with intensity of 50–55% maximum speed for three sessions per week and groups 3 and 4 received 25 mg/kg/day crocin peritoneally.
Shapiro–Wilk, one-way ANOVA with Tukey’s post-hot tests were used for statistical analysis of data (P ≤ 0.05). DM induction significantly increased Bcl-2 as
well as decreased Bax and P52 (P ≤ 0.05) nevertheless training and training with
crocin significantly decreased Bcl-2 and increased Bax and P53 (P ≤ 0.05); crocin
significantly decreased Bcl-2 and increased P53 (P ≤ 0.05) and training with crocin had higher effect on increase of Bax and P53 compare to training (P ≤ 0.05)
also increase of Bax compare to crocin. Although training and crocin alone can
improve apoptotic markers in diabetic rats, nevertheless training simultaneously
with crocin have better effects than training alone. 相似文献
986.
Farhadi Tayebeh Hashemian Seyed MohammadReza Farhadi Zinat 《International journal of peptide research and therapeutics》2020,26(2):1061-1071
International Journal of Peptide Research and Therapeutics - Acinetobacter infection is one of the main causes of mortality in the patients admitted to the intensive care units (ICUs). Activation... 相似文献
987.
988.
989.
Samaneh Rezaei Maryam Mahjoubin Tehran Amirhossein Sahebkar Amin Jalili Seyed Hamid Aghaee-Bakhtiari PhD 《Journal of cellular physiology》2020,235(4):3222-3234
Prostate cancer (PCa) is one of the most common cancers and the fifth most common reason for cancer deaths in the males. Surgical castration combined with androgen deprivation therapy, antiandrogens, and androgen synthesis inhibitors is the current therapeutic modalities for PCa. These strategies inhibit androgen synthesis or reduce its binding to the androgen receptor (AR) but the development of resistance to these therapies and transient responsiveness are challenging issues in the treatment of this cancer. Deregulation of ARs has a vital role in the initiation and progression of PCa. Also, recent findings imply that micro RNAs (miRNAs) are involved in the evolution of PCa and mediate drug resistance in different cancers. Hence, discovering and targeting miRNAs might represent a novel therapeutic approach. This review paid particular attention to the AR pathway and existing information on the possible roles of miRNAs associated with AR pathway and drug resistance to two second-generation antiandrogens, that is, enzalutamide and abiraterone. 相似文献