全文获取类型
收费全文 | 15939篇 |
免费 | 795篇 |
国内免费 | 21篇 |
专业分类
16755篇 |
出版年
2023年 | 111篇 |
2022年 | 273篇 |
2021年 | 421篇 |
2020年 | 253篇 |
2019年 | 256篇 |
2018年 | 398篇 |
2017年 | 430篇 |
2016年 | 495篇 |
2015年 | 672篇 |
2014年 | 742篇 |
2013年 | 1037篇 |
2012年 | 1142篇 |
2011年 | 1039篇 |
2010年 | 626篇 |
2009年 | 571篇 |
2008年 | 657篇 |
2007年 | 649篇 |
2006年 | 564篇 |
2005年 | 475篇 |
2004年 | 448篇 |
2003年 | 379篇 |
2002年 | 346篇 |
2001年 | 334篇 |
2000年 | 305篇 |
1999年 | 244篇 |
1998年 | 125篇 |
1997年 | 106篇 |
1996年 | 96篇 |
1995年 | 112篇 |
1994年 | 101篇 |
1993年 | 95篇 |
1992年 | 221篇 |
1991年 | 207篇 |
1990年 | 200篇 |
1989年 | 193篇 |
1988年 | 168篇 |
1987年 | 164篇 |
1986年 | 148篇 |
1985年 | 174篇 |
1984年 | 167篇 |
1983年 | 115篇 |
1982年 | 114篇 |
1981年 | 100篇 |
1980年 | 92篇 |
1979年 | 147篇 |
1978年 | 109篇 |
1977年 | 95篇 |
1974年 | 118篇 |
1973年 | 91篇 |
1972年 | 100篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
141.
Isolation of cellulolytic mutants of thermotolerant fungus Chaetomium cellulolyticum ATCC 32319 总被引:1,自引:0,他引:1
Spores of Chaetomium cellulolyticum were treated with 200 micrograms/ml of N-methyl-N'-nitro-N-nitrosoguanidine and seven mutants producing clear zones around their colonies on modified Vogels medium were isolated. Mutant NG7 showed altered morphological characteristics and produced more cellulases (CMCase--15 units, FPA--6.5 units, CDA--0.80 units and cellobiase--4.7 units/ml) than its parental strain (CMCase--10 units, FPA--4.5 units, CDA--0.36 units and cellobiase--2.7 units/ml). Cellulase preparation was used to saccharify rice straw, wheat straw, bagasse and sawdust, pretreated with 1% sodium hydroxide. 相似文献
142.
143.
Subunit structure of human and rat glutathione S-transferases 总被引:4,自引:0,他引:4
Y C Awasthi S V Singh 《Comparative biochemistry and physiology. B, Comparative biochemistry》1985,82(1):17-23
In rat tissues different forms of glutathione (GSH) S-transferases represent various dimeric combinations of at least four different classes of subunits categorized on the basis of their Mr values as seen on polyacrylamide gels. These subunit types represent heterogeneous populations and the actual number of subunits in rat GSH S-transferases may be far more than is known at present. Human GSH S-transferases arise from dimeric combinations of at least four immunologically and functionally distinct subunits which can be classified into three types, A (Mr 26,500), B (Mr 24,500) and C (Mr 22,500). There is evidence for considerable charge heterogeneity in each of these subunit types. 相似文献
144.
Dacus cucurbitae is a serious pest of various types of fleshy fruits and vegetables. The mitotic and salivary chromosomes were reinvestigated using the air-drying and different (C-and H-) banding techniques with a view to rectify the existing controversy regarding the Indian populations of this species. A standard map of its salivary chromosomes was constructed and some important identifiable landmarks were recognized in each arm. 相似文献
145.
Emergence of some caddisflies (Trichoptera) from a wooded stream in southern Ontario 总被引:1,自引:1,他引:0
Twenty-eight species of nine families of caddisflies (Trichoptera) were identified in 170 samples taken over an 8-month period from five emergence traps placed on a second-order, forested, cold-stenothermal stream on the Niagara Escarpment, Ontario, Canada.A mean of 980.9 caddisflies m–2 of streambed was obtained over the entire sampling period. Eleven common species accounted for 92.8% of the total emergence with specific proportions ranging from 23.8% (Wormaldia moesta) to 0.11 % (Rhyacophila sp.). The use of various kinds of traps in other studies and their effects on the detection of species composition and abundance are discussed and compared with the present study.Although the distributions of all the common species were invariant over time, four species showed low to high degrees of patchiness in the streambed; the other seven common species were uniformly distributed. However, a large residual variance suggested a subtle mechanism of microhabitat selection by the larvae and (or) pupae, not detectable by even the small emergence traps used.Both sexes of 15 species, only males of 4 and only females of 9 species were collected. Eight of the eleven common species showed significant departures from a balanced sex ratio and five exhibited a protandry of from 1 to 3 weeks. Neither this study nor others have been able to establish a predictable pattern of sex ratios in Trichoptera.The emergence periods and patterns of the eleven common species are described and compared with other studies. Of these common species, one emerged in the spring, seven during the summer and three during the late summer or early fall. Ten species had a short emergence period with a distinct peak and a significantly skewed pattern. One species,Parapsyche apicalis, exhibited a prolonged emergence period, no distinct peak and a significantly platykurtotic2 pattern. With the exception ofLepidostoma sp. A, the emergence patterns of the common species were unimodal. 相似文献
146.
Planococcus citri (Risso) is one of the major pests of citrus orchards in India. For the control ofP. citri, an encyrtid parasite,Leptomastix dactylopii How. was introduced from West Indies in 1983. The parasite was mass bred and inoculative releases were made in 2 selected
citrus orchards where infestation of mealybug on fruits (sweet orange, seedless lime and acid lime) ranged from 38 to 65 per
cent. Establishment of the parasite in the 2 release orchards resulted in complete control of the mealybug within 3 to 4 months.
No insecticidal sprays were required subsequently for the control ofP. citri in the following seasons.
Contribution No. 152/85 of the IIHR, Bangalore — 560089. 相似文献
147.
A. K. Singh 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1986,72(4):433-439
Summary Synthetic amphidiploids were established in 32 combinations involving 8 diploid wild species representing both A and B genomes of section Arachis. Bivalent and multivalent associations in the amphidiploids of 7 A genome species confirm that these species have identical genomes. Contrastingly, high bivalent frequencies in amphidiploids involving the A and B genome species suggest that A. batizocoi has a distinct B genome that is partially homologous to the other genome A represented in the rest of the species. Crossability, chromosome pairing and pollen and pod fertility in hybrids between A. hypogaea and amphidiploids have revealed that these amphidiploids can be used as a genetic bridge for the transfer of genes from the wild species into the cultivated groundnut.Submitted as Journal Article No. 530 by International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) 相似文献
148.
B. N. Dahiya V. P. Singh 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1986,72(5):678-681
Summary Three selection methods (single seed descent (SSD), mass selection and selective intermating) were applied simultaneously to a highly heterogeneous and broadly based population of greengram. Progeny developing after two cycles of selection were evaluated for yield and seven other economic characters. The relative efficacy of each selection method was judged on the basis of the number of high yielding progeny, mean yield of top 10% progeny, and mean of the highest yielding progeny. Selection after two cycles of selective intermating was found to be the best method for generating productive progeny although mass selection favouring smaller seeds was an equally efficient method. Both of these were found superior to SSD selection. 相似文献
149.
150.
Exposure of enterotoxigenic Escherichia coli strains to a sublethal concentration (0.75 mg/liter) of copper for 3 days at 4 degrees C induced sensitivity to deoxycholate (0.1%). When placed in a complex (brain heart infusion) or a defined amino acid salt medium, the copper-injured cells recovered their tolerance to deoxycholate in 3 and 6 h, respectively, and commenced active growth. Growth and heat-stable enterotoxin production of uninjured and copper-injured cells were studied in brain heart infusion medium. A slightly altered growth curve and an initial slow rate of toxin production were observed in injured cells when compared with those corresponding uninjured controls. However, maximum heat-stable enterotoxin levels in injured cultures were comparable to those produced by uninjured cells, suggesting that the enterotoxigenic potential of copper-injured cells was fully retained. 相似文献