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排序方式: 共有236条查询结果,搜索用时 15 毫秒
31.
Giuseppe Bianco Bianca Fontanella Lorella Severino Andrea Quaroni Giuseppina Autore Stefania Marzocco 《PloS one》2012,7(12)
The integrity of the gastrointestinal tract represents a crucial first level defence against ingested toxins. Among them, Nivalenol is a trichotecenes mycotoxin frequently found on cereals and processed grains; when it contaminates human food and animal feed it is often associated with another widespread contaminant, Deoxynivalenol. Following their ingestion, intestinal epithelial cells are exposed to concentrations of these trichothecenes high enough to cause mycotoxicosis. In this study we have investigated the effects of Nivalenol and Deoxynivalenol on intestinal cells in an in vitro model system utilizing the non-tumorigenic rat intestinal epithelial cell line IEC-6. Both Nivalenol and Deoxynivalenol (5–80 µM) significantly affected IEC-6 viability through a pro-apoptotic process which mainly involved the following steps: (i) Bax induction; (ii) Bcl-2 inhibition, and (iii) caspase-3 activation. Moreover, treatment with Nivalenol produced a significant cell cycle arrest of IEC-6 cells, primarily at the G0/G1 interphase and in the S phase, with a concomitant reduction in the fraction of cells in G2. Interestingly, when administered at lower concentrations (0.1–2.5 µM), both Nivalenol and Deoxynivalenol affected epithelial cell migration (restitution), representing the initial step in gastrointestinal wound healing in the gut. This reduced motility was associated with significant remodelling of the actin cytoskeleton, and changes in expression of connexin-43 and focal adhesion kinase. The concentration range of Nivalenol or Deoxynivalenol we have tested is comparable with the mean estimated daily intake of consumers eating contaminated food. Thus, our results further highlight the risks associated with intake of even low levels of these toxins. 相似文献
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33.
F Fiorino M Eiden A Giese B Severino A Esposito MH Groschup E Perissutti E Magli GM Incisivo A Ciano F Frecentese HA Kretzschmar J Wagner V Santagada G Caliendo 《Bioorganic & medicinal chemistry》2012,20(16):5001-5011
A new set of 5-(2-(pyrrolidin-1-yl)acetamido)-N-butyl-2-(substituted)benzamide and 5-(2-(piperidin-1-yl)acetamido)-N-butyl-2-(substituted) benzamide derivatives were synthesized in which as structural features the 2-(1-pyrrolidinyl)- or 2-(1-piperidyl)acetylamino group or a diphenylether moiety are associated to a benzamide scaffold. Their binding affinity for human PrP(C) and inhibition of its conversion into PrP(Sc) were determined in vitro; moreover, the antiprion activity was assayed by inhibition of PrP(Sc) accumulation in scrapie-infected mouse neuroblastoma cells (ScN2a) and scrapie mouse brain (SMB) cells. The results clearly indicate the benzamide derivatives as attractive lead compounds for the development of potential therapeutic agents against prion disease. 相似文献
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35.
Nonnis S Cappelletti G Taverna F Ronchi C Ronchi S Negri A Grassi E Tedeschi G 《Neurochemical research》2008,33(3):518-525
Nitration of tau protein is normally linked to neurodegeneration but, until now, no comprehensive information is available
regarding tau nitration in healthy subjects. It has been previously reported that in differentiated PC12 cells, tau co-immunoprecipitated
with alpha-tubulin is nitrated at tyrosine residues and that this post-translation modification doesn’t impair the association
of tau with the cytoskeleton. The present paper is focused on the identification of tyrosine residues endogenously modified
in tau from PC12 cells and reports for the first time that tau is also nitrated in vivo in normal mouse brain and that one
tyrosine is endogenously modified. 相似文献
36.
Plant Molecular Biology - 相似文献
37.
Esposito Ciro Roberti Agnese Turrà Francesco Cerulo Mariapina Severino Giovanni Settimi Alessandro Escolino Maria 《Probiotics and antimicrobial proteins》2018,10(2):323-328
Probiotics and Antimicrobial Proteins - This study aimed to evaluate the effectiveness of probiotics (Lactobacillus rhamnosus GG), as a preventive measure of antibiotic-associated diarrhea (AAD) in... 相似文献
38.
Patricia Cano-Sanchez Beatrice Severino V V Sureshbabu Joe Russo Tatsuya Inui Fa-Xiang Ding Boris Arshava Jeff Becker Fred Naider 《Journal of peptide science》2006,12(12):808-822
Transmembrane domains (TMDs) of G-protein coupled receptors (GPCRs) have very low water solubility and often aggregate during purification and biophysical investigations. To circumvent this problem many laboratories add oligolysines to the N- and C-termini of peptides that correspond to a TMD. To systematically evaluate the effect of the oligolysines on the biophysical properties of a TMD we synthesized 21 peptides corresponding to either the second (TPIFIINQVSLFLIILHSALYFKY) or sixth (SFHILLIMSSQSLLVPSIIFILAYSLK) TMD of Ste2p, a GPCR from Saccharomyces cerevisiae. Added to the termini of these peptides were either Lys(n) (n = 1,2,3) or the corresponding native loop residues. The biophysical properties of the peptides were investigated by circular dichroism (CD) spectroscopy in trifluoroethanol-water mixtures, sodium dodecyl sulfate (SDS) micelles and dimyristoylphosphocholine (DMPC)-dimyristoylphosphoglycerol (DMPG) vesicles, and by attenuated total reflection Fourier transform infrared (ATR-FTIR) in DMPC/DMPG multilayers. The results show that the conformation assumed depends on the number of lysine residues and the sequence of the TMD. Identical peptides with native or an equal number of lysine residues exhibited different biophysical properties and structural tendencies. 相似文献
39.
Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A‐Induced Nephrotoxicity
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