全文获取类型
收费全文 | 6997篇 |
免费 | 461篇 |
国内免费 | 2篇 |
专业分类
7460篇 |
出版年
2023年 | 42篇 |
2022年 | 102篇 |
2021年 | 223篇 |
2020年 | 133篇 |
2019年 | 149篇 |
2018年 | 226篇 |
2017年 | 178篇 |
2016年 | 231篇 |
2015年 | 331篇 |
2014年 | 421篇 |
2013年 | 481篇 |
2012年 | 557篇 |
2011年 | 567篇 |
2010年 | 342篇 |
2009年 | 299篇 |
2008年 | 396篇 |
2007年 | 418篇 |
2006年 | 381篇 |
2005年 | 343篇 |
2004年 | 296篇 |
2003年 | 292篇 |
2002年 | 241篇 |
2001年 | 88篇 |
2000年 | 74篇 |
1999年 | 64篇 |
1998年 | 53篇 |
1997年 | 38篇 |
1996年 | 26篇 |
1995年 | 41篇 |
1994年 | 33篇 |
1993年 | 43篇 |
1992年 | 30篇 |
1991年 | 26篇 |
1990年 | 31篇 |
1989年 | 25篇 |
1988年 | 15篇 |
1987年 | 19篇 |
1986年 | 18篇 |
1985年 | 23篇 |
1984年 | 21篇 |
1983年 | 10篇 |
1982年 | 15篇 |
1981年 | 12篇 |
1979年 | 12篇 |
1977年 | 10篇 |
1976年 | 10篇 |
1975年 | 10篇 |
1974年 | 8篇 |
1972年 | 8篇 |
1970年 | 7篇 |
排序方式: 共有7460条查询结果,搜索用时 15 毫秒
81.
Oliva ML Santomauro-Vaz EM Andrade SA Juliano MA Pott VJ Sampaio MU Sampaio CA 《Biological chemistry》2001,382(1):109-113
We have previously described Kunitz-type serine proteinase inhibitors purified from Bauhinia seeds. Human plasma kallikrein shows different susceptibility to those inhibitors. In this communication, we describe the interaction of human plasma kallikrein with fluorogenic and non-fluorogenic peptides based on the Bauhinia inhibitors' reactive site. The hydrolysis of the substrate based on the B. variegata inhibitor reactive site sequence, Abz-VVISALPRSVFIQ-EDDnp (Km 1.42 microM, kcat 0.06 s(-1), and kcat/Km 4.23 x 10(4) M(-1) s(-1)), is more favorable than that of Abz-VMIAALPRTMFIQ-EDDnp, related to the B. ungulata sequence (Km 0.43 microM, kcat 0.00017 s(-1), and kcat/Km 3.9 x 10(2) M(-1) s(-1)). Human plasma kallikrein does not hydrolyze the substrates Abz-RPGLPVRFESPL-EDDnp and Abz-FESPLRINIIKE-EDDnp based on the B. bauhinioides inhibitor reactive site sequence, the most effective inhibitor of the enzyme. These peptides are competitive inhibitors with Ki values in the nM range. The synthetic peptide containing 19 amino acids based on the B. bauhinioides inhibitor reactive site (RPGLPVRFESPL) is poorly cleaved by kallikrein. The given substrates are highly specific for trypsin and chymotrypsin hydrolysis. Other serine proteinases such as factor Xa, factor XII, thrombin and plasmin do not hydrolyze B. bauhinioides inhibitor related substrates. 相似文献
82.
83.
Sergio Sgorbati Marisa Levi Elio Sparvoli Federica Trezzi Giovanni Lucchini 《Physiologia plantarum》1986,68(3):471-476
Cytometry and flow cytometry were used to study characteristics of fluorescence of the DNA-DAPI complex in nuclei released from different fresh and formaldehyde-fixed pea ( Pisum sativum L. cv. Lincoln) tissues. The two methods of isolation are compared and discussed as well as their possible use for quantitative analysis of DNA in plant tissues. With fixed tissues it is possible to obtain a number of nuclei sufficient for the flow cytometric analysis, even using small amounts of plant tissue. 相似文献
84.
In the nurse plant syndrome, or nurse association, seedlings (beneficiaries) are associated with adult shrubs/trees (benefactors).
This phenomenon has been documented in several regions of the planet. Abiotic stress amelioration (one mechanism of facilitation)
is one of the causes of this association. Most of the studies addressing the nurse syndrome have been conducted on spatial
scales of a few hectares and have focused on only one or a few species. Moreover, there is an almost complete lack of studies
addressing the incidence and characteristics of the nurse phenomenon in the arid Andes of South America. We undertook a first
approximation to the study of facilitation in these ecosystems. The study was conducted at local and regional scales and involved
the assessment of the spatial distribution of juveniles (seedlings and saplings) of 51 populations of 16 shrub and 12 cactus
species in relation to shrub cover at 20 localities of the Prepuna (subtropical Andes of Bolivia and Argentina, 20–26°S).
In terms of spatial distribution, the juveniles of most of the populations of shrubs studied were distributed both under the
shrubs and in open spaces, thereby showing an apparent indifference to microhabitat. Globose and opuntioid cacti were preferentially
distributed below the canopies of shrubs and were usually more associated with the dominant shrub species, which stood out
as better potential nurses. The pattern was consistent throughout the region, including the more mesic and arid localities.
The fact that Prepuna woody species are capable of establishing in open spaces would confer this region a greater resilience.
Our findings further suggest that community dynamics in arid and semi-arid environments are more variable than previously
thought. 相似文献
85.
86.
Pasquale Ferranti Sergio Lilla Lina Chianese Francesco Addeo 《The protein journal》1999,18(5):595-602
Multiple forms of αs1-casein were identified in the four major ruminant species by structural characterization of the protein fraction. While αs1-casein phenotypes were constituted by a mixture of at least seven molecular forms in ovine and caprine species, there were only two forms in bovine and water buffalo species. In ovine and caprine forms the main component corresponded to the 199-residue-long form, and the deleted proteins differed from the complete one by the absence of peptides 141–148, 110–117, or Gln78, or a combination of such deletions. The deleted segments corresponded to the sequence regions encoded by exons 13 and 16, and by the first triplet of exon 11 (CAG), suggesting that the occurrence of the short protein forms is due to alternative skipping, as previously demonstrated for some caprine and ovine phenotypes. The alternative deletion of Gln78 in αs1-casein, the only form common to the milk of all the species examined and located in a sequence region joining the polar phosphorylation cluster and the hydrophobic C-terminal domain of the protein, may play a functional role in the stabilization of the milk micelle structure. 相似文献
87.
DC Andrade C Henriquez–Olguín AR Beltrán MA Ramírez C Labarca M Cornejo C álvarez R Ramírez-Campillo 《Biology of sport / Institute of Sport》2015,32(2):123-128
The purpose of this study was to compare the acute effects of general, specific and combined warm-up (WU) on explosive performance. Healthy male (n = 10) subjects participated in six WU protocols in a crossover randomized study design. Protocols were: passive rest (PR; 15 min of passive rest), running (Run; 5 min of running at 70% of maximum heart rate), stretching (STR; 5 min of static stretching exercise), jumping [Jump; 5 min of jumping exercises – 3x8 countermovement jumps (CMJ) and 3x8 drop jumps from 60 cm (DJ60)], and combined (COM; protocols Run+STR+Jump combined). Immediately before and after each WU, subjects were assessed for explosive concentric-only (i.e. squat jump – SJ), slow stretch-shortening cycle (i.e. CMJ), fast stretch-shortening cycle (i.e. DJ60) and contact time (CT) muscle performance. PR significantly reduced SJ performance (p =0.007). Run increased SJ (p =0.0001) and CMJ (p =0.002). STR increased CMJ (p =0.048). Specific WU (i.e. Jump) increased SJ (p =0.001), CMJ (p =0.028) and DJ60 (p =0.006) performance. COM increased CMJ performance (p =0.006). Jump was superior in SJ performance vs. PR (p =0.001). Jump reduced (p =0.03) CT in DJ60. In conclusion, general, specific and combined WU increase slow stretch-shortening cycle (SSC) muscle performance, but only specific WU increases fast SSC muscle performance. Therefore, to increase fast SSC performance, specific fast SSC muscle actions must be included during the WU. 相似文献
88.
José David Flores-Félix Luis R. Silva Lina P. Rivera Marta Marcos-García Paula García-Fraile Eustoquio Martínez-Molina Pedro F. Mateos Encarna Velázquez Paula Andrade Raúl Rivas 《PloS one》2015,10(4)
The increasing interest in the preservation of the environment and the health of consumers is changing production methods and food consumption habits. Functional foods are increasingly demanded by consumers because they contain bioactive compounds involved in health protection. In this sense biofertilization using plant probiotics is a reliable alternative to the use of chemical fertilizers, but there are few studies about the effects of plant probiotics on the yield of functional fruits and, especially, on the content of bioactive compounds. In the present work we reported that a strain of genus Phyllobacterium able to produce biofilms and to colonize strawberry roots is able to increase the yield and quality of strawberry plants. In addition, the fruits from plants inoculated with this strain have significantly higher content in vitamin C, one of the most interesting bioactive compounds in strawberries. Therefore the use of selected plant probiotics benefits the environment and human health without agronomical losses, allowing the production of highly functional foods. 相似文献
89.
90.
The stability of Pseudomonas putida F1, a strain harbouring the genes responsible for toluene degradation in the chromosome was evaluated in a bioscrubber under
high toluene loadings and nitrogen limiting conditions at two dilution rates (0.11 and 0.27 h−1). Each experiment was run for 30 days, period long enough for microbial instability to occur considering previously reported
studies carried out with bacterial strains encoding the catabolic genes in the TOL plasmid. At all tested conditions, P. putida F1 exhibited stable performance as shown by the constant values of the specific toluene degradation yield, CO2 produced versus toluene degraded yield, and biomass concentration within each steady state. Benzyl alcohol, a curing agent
causing TOL plasmid deletion in Pseudomonas strains, was present in the cultivation medium as a result of the monooxygenation of toluene by the diooxygenase system of
P. putida F1. However, no mutant population growing at the expense of the extracellular excreted carbon or lysis products was established
in the chemostat as confirmed by the constant dissolved total organic carbon (TOC) concentration and fraction of toluene degrading
cells (approx. 100%). In addition, batch experiments conducted with both lysis substrate and toluene simultaneously confirmed
that P. putida F1 preferentially consumed toluene rather than extracellular excreted carbon. 相似文献