全文获取类型
收费全文 | 5013篇 |
免费 | 343篇 |
国内免费 | 2篇 |
专业分类
5358篇 |
出版年
2023年 | 25篇 |
2022年 | 59篇 |
2021年 | 153篇 |
2020年 | 90篇 |
2019年 | 95篇 |
2018年 | 149篇 |
2017年 | 116篇 |
2016年 | 154篇 |
2015年 | 224篇 |
2014年 | 290篇 |
2013年 | 357篇 |
2012年 | 411篇 |
2011年 | 432篇 |
2010年 | 253篇 |
2009年 | 234篇 |
2008年 | 284篇 |
2007年 | 328篇 |
2006年 | 300篇 |
2005年 | 252篇 |
2004年 | 233篇 |
2003年 | 224篇 |
2002年 | 192篇 |
2001年 | 40篇 |
2000年 | 32篇 |
1999年 | 32篇 |
1998年 | 39篇 |
1997年 | 27篇 |
1996年 | 16篇 |
1995年 | 35篇 |
1994年 | 25篇 |
1993年 | 31篇 |
1992年 | 19篇 |
1991年 | 14篇 |
1990年 | 20篇 |
1989年 | 14篇 |
1988年 | 11篇 |
1987年 | 9篇 |
1986年 | 12篇 |
1985年 | 13篇 |
1984年 | 15篇 |
1983年 | 9篇 |
1982年 | 13篇 |
1981年 | 11篇 |
1979年 | 9篇 |
1978年 | 5篇 |
1977年 | 9篇 |
1976年 | 7篇 |
1975年 | 6篇 |
1972年 | 4篇 |
1970年 | 7篇 |
排序方式: 共有5358条查询结果,搜索用时 15 毫秒
51.
Ailec Ho‐Plagaro Concepcin Santiago‐Fernandez Cristina Rodríguez‐Díaz Carlos Lopez‐Gmez Sara Garcia‐Serrano Francisca Rodríguez‐Pacheco Sergio Valdes Alberto Rodríguez‐Caete Guillermo Alcaín‐Martínez Natalia Ruiz‐Santana Luis Vzquez‐Pedreo Eduardo García‐Fuentes 《Obesity (Silver Spring, Md.)》2020,28(9):1708-1717
52.
Julian Padr Sergio A. Lambertucci Paula L. Perrig Jonathan N. Pauli 《Ecology and evolution》2020,10(23):13011
While genetic diversity of threatened species is a major concern of conservation biologists, historic patterns of genetic variation are often unknown. A powerful approach to assess patterns and processes of genetic erosion is via ancient DNA techniques. Herein, we analyzed mtDNA from historical samples (1800s to present) of Andean Condors (Vultur gryphus) to investigate whether contemporary low genetic variability is the result of recent human expansion and persecution, and compared this genetic history to that of California condors (Gymnogyps californianus).We then explored historic demographies for both species via coalescent simulations. We found that Andean condors have lost at least 17% of their genetic variation in the early 20th century. Unlike California condors, however, low mtDNA diversity in the Andean condor was mostly ancient, before European arrival. However, we found that both condor species shared similar demographies in that population bottlenecks were recent and co‐occurred with the introduction of livestock to the Americas and the global collapse of marine mammals. Given the combined information on genetic and demographic processes, we suggest that the protection of key habitats should be targeted for conserving extant genetic diversity and facilitate the natural recolonization of lost territories, while nuclear genomic data should be used to inform translocation plans. 相似文献
53.
54.
Manuella Maria Silva Santos Damián Gatica Jaqueline de Azêvedo Silva Sergio Crovella Daniel J. Klionsky Marcos Antonio De Morais Jr 《生物化学与生物物理学报:疾病的分子基础》2021,1867(4):166053
Mevalonate kinase deficiency (MKD) is an autosomal recessive disorder in humans that causes systemic autoinflammatory problems to children. Previously, we used a yeast model to show that MKD results in mitochondrial malfunctioning that may finally induce mitophagy. Here, we proved that MKD indeed induced general autophagy as well as mitophagy in yeast, but these mechanisms did not go to completion. Therefore, the limitation of mevalonate kinase activity produces dysfunctional mitochondria that might not be recycled, causing metabolic dysfunctions in the cells. Understanding this mechanism may provide a piece in solving the nonspecific autoinflammatory response puzzle observed in MKD patients. 相似文献
55.
Mayarling Francisca Troncoso Jafet Ortiz-Quintero Valeria Garrido-Moreno Fernanda Sanhueza-Olivares Alejandra Guerrero-Moncayo Mario Chiong Pablo F. Castro Lorena García Luigi Gabrielli Ramón Corbalán Luis Garrido-Olivares Sergio Lavandero 《生物化学与生物物理学报:疾病的分子基础》2021,1867(9):166170
The vascular cellular adhesion molecule-1 (VCAM-1) is a protein that canonically participates in the adhesion and transmigration of leukocytes to the interstitium during inflammation. VCAM-1 expression, together with soluble VCAM-1 (sVCAM-1) induced by the shedding of VCAM-1 by metalloproteinases, have been proposed as biomarkers in immunological diseases, cancer, autoimmune myocarditis, and as predictors of mortality and morbidity in patients with chronic heart failure (HF), endothelial injury in patients with coronary artery disease, and arrhythmias. This revision aims to discuss the role of sVCAM-1 as a biomarker to predict the occurrence, development, and preservation of cardiovascular disease. 相似文献
56.
Anaya-Ramos Laura Díaz-Ruíz Araceli Ríos Camilo Mendez-Armenta Marisela Montes Sergio Aguirre-Vidal Yoshajandith García-Jiménez Sara Baron-Flores Veronica Monroy-Noyola Antonio 《Biometals》2021,34(6):1295-1311
BioMetals - Thallium (TI) is one of the most toxic heavy metals. Human exposure to Tl occurs through contaminated drinking water and from there to food, a threat to health. Recently, environmental... 相似文献
57.
58.
59.
Marta Cambra Sergio Madrigal-Mora Isaac Chinchilla Geiner Golfín-Duarte Christopher G. Lowe Mario Espinoza 《Journal of fish biology》2021,99(3):1140-1144
Tiger sharks (Galeocerdo cuvier) play an important ecological role as top predators, yet knowledge of their reproductive ecology is scarce. Here, the authors report the first observation of a potential neonate G. cuvier at Cocos Island, a predator-dominated oceanic island in the Eastern Tropical Pacific (ETP). The individual was detected using baited remote underwater video stations (BRUVS). The cameras also detected female individuals potentially pregnant, suggesting that parturition may take place at or near the island. Nonetheless, it is still unclear if the presence of a single neonate is an isolated event or evidence that the species is using the island for reproduction. 相似文献
60.
Alberto Cornejo Julio Caballero Mario Simirgiotis Vanessa Torres Luisa Snchez Nicols Díaz Marcela Guimaraes Marcos Hernndez Carlos Areche Sergio Alfaro Leonardo Caballero Francisco Melo 《Journal of enzyme inhibition and medicinal chemistry》2021,36(1):154
Parkinson''s disease (PD) is a neurodegenerative disorder that affects adult people whose treatment is palliative. Thus, we decided to test three dammarane triterpenes 1, 1a, 1b, and we determined that 1 and 1a inhibit β-aggregation through thioflavine T rather than 1b. Since compound 1 was most active, we determined the interaction between α-synuclein and 1 at 50 µM (Kd) through microscale thermophoresis. Also, we observed differences in height and diameter of aggregates, and α-synuclein remains unfolded in the presence of 1. Also, aggregates treated with 1 do not provoke neurites'' retraction in N2a cells previously induced by retinoic acid. Finally, we studied the potential sites of interaction between 1 with α-synuclein fibrils using molecular modelling. Docking experiments suggest that 1 preferably interact with the site 2 of α-synuclein through hydrogen bonds with residues Y39 and T44. 相似文献