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921.
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Alexeev D Kostrjukova E Aliper A Popenko A Bazaleev N Tyakht A Selezneva O Akopian T Prichodko E Kondratov I Chukin M Demina I Galyamina M Kamashev D Vanyushkina A Ladygina V Levitskii S Lazarev V Govorun V 《Journal of proteome research》2012,11(1):224-236
To date, no genome of any of the species from the genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current genome sequencing technologies. Here, we report the assembly of the Spiroplasma melliferum KC3 genome into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on the discovery of 521 expressed proteins and comprehensive metabolomic profiling. A systems approach allowed us to elucidate putative pathogenicity mechanisms and to discover major virulence factors, such as Chitinase utilization enzymes and toxins never before reported for insect pathogenic spiroplasmas. 相似文献
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Evgeniy V. Dubrovin Anastasia V. Popova Sergey V. Kraevskiy Sergei G. Ignatov Tatyana E. Ignatyuk Igor V. Yaminsky Nikolay V. Volozhantsev 《PloS one》2012,7(10)
Background
Acinetobacter baumannii is known for its ability to develop resistance to the major groups of antibiotics, form biofilms, and survive for long periods in hospital environments. The prevalence of infections caused by multidrug-resistant A. baumannii is a significant problem for the modern health care system, and application of lytic bacteriophages for controlling this pathogen may become a solution.Methodology/Principal Findings
In this study, using atomic force microscopy (AFM) and microbiological assessment we have investigated A. baumannii bacteriophage AP22, which has been recently described. AFM has revealed the morphology of bacteriophage AP22, adsorbed on the surfaces of mica, graphite and host bacterial cells. Besides, morphological changes of bacteriophage AP22-infected A. baumannii cells were characterized at different stages of the lytic cycle, from phage adsorption to the cell lysis. The phage latent period, estimated from AFM was in good agreement with that obtained by microbiological methods (40 min). Bacteriophage AP22, whose head diameter is 62±1 nm and tail length is 88±9 nm, was shown to disperse A. baumannii aggregates and adsorb to the bacterial surface right from the first minute of their mutual incubation at 37°C.Conclusions/Significance
High rate of bacteriophage AP22 specific adsorption and its ability to disperse bacterial aggregates make this phage very promising for biomedical antimicrobial applications. Complementing microbiological results with AFM data, we demonstrate an effective approach, which allows not only comparing independently obtained characteristics of the lytic cycle but also visualizing the infection process. 相似文献926.
The large Southeast Asian genus Desmoxytes is slightly rediagnosed. A number of troglomorphic, most likely troglobitic, species occur in southern China. A key is provided to all 10 Desmoxytes spp. currently known from China, including three new presumed troglobites: Desmoxytes eupterygotasp. n. from Hunan Province, as well as Desmoxytes spinissimasp. n. and Desmoxytes luisp. n. from Guangxi Province. "Desmoxytes" philippina Nguyen Duc & Sierwald, 2010, from the Philippines, is formally removed from Desmoxytes, but not assigned to another genus. It probably belongs in a new genus in the subfamily Australiosomatinae, tribe Antichiropodini, close to the Bornean Euphyodesmus Attems, 1931 and Borneochiropus Golovatch, 1996. 相似文献
927.
Boudko SP Engel J Bächinger HP 《The international journal of biochemistry & cell biology》2012,44(1):21-32
Collagens contain large numbers of Gly-Xaa-Yaa peptide repeats that form the characteristic triple helix, where the individual chains fold into a polyproline II helix and three of these helices form a right-handed triple helix. For the proper folding of the triple helix collagens contain trimerization domains. These domains ensure a single starting point for triple helix formation and are also responsible for the chain selection in heterotrimeric collagens. Trimerization domains are non-collagenous domains of very different structures. The size of trimerization domains varies from 35 residues in type IX collagen to around 250 residues for the fibrillar collagens. These domains are not only crucial for biological functions, but they are also attractive tools for generating recombinant collagen fragments of interest as well as for general use in protein engineering and biomaterial design. Here we review the current knowledge of the structure and function of these trimerization domains. 相似文献
928.
Platonova A Koltsova SV Hamet P Grygorczyk R Orlov SN 《Apoptosis : an international journal on programmed cell death》2012,17(5):429-438
Contrasting cell volume behaviours (swelling vs. shrinkage) are considered as criteria to distinguish necrosis from apoptosis.
In this study, we employed a time-lapse, dual-image surface reconstruction technique to assess the volume of single vascular
smooth muscle cells transfected with E1A-adenoviral protein (E1A-VSMC) and undergoing rapid apoptosis in the absence of growth
factors or in the presence of staurosporine. After 30- to 60-min lag-phase, serum-deprived E1A-VSMC volume was increased by
~40%, which preceded maximal increments of caspase-3 activity and chromatin cleavage. Swollen cells underwent rapid apoptotic
collapse, documented by plasma membrane budding, and terminated in 10–15 min by the formation of numerous apoptotic bodies.
Suppression of apoptosis by inhibition of Na+,K+-ATPase and activation of cAMP signalling with ouabain and forskolin, respectively, completely abolished the swelling of serum-deprived
E1A-VSMC. In contrast to serum deprivation, apoptotic collapse of staurosporine-treated E1A-VSMC preceded attenuation of their
volume by ~30%. Neither transient hyposmotic swelling nor isosmtotic shrinkage triggered apoptosis. Our results show that
cell shrinkage can not be considered as ubiquitous hallmark of apoptosis. The involvement of stimulus-specific cell volume
perturbations in initiation and progression of apoptosis in vascular smooth muscle cells should be examined further. 相似文献
929.
Viktor I. Lyamichev Sergei M. Mirkin Maxim D. Frank-Kamenetskii 《Journal of biomolecular structure & dynamics》2013,31(2):275-282
Abstract We have recently shown that a (GA)n · (TC)n tract undergoes a sharp structural transition under superhelical stress (V.I. Lyamichev, S.M. Mirkin and M.D. Frank-Kamenetskii, J. Biomol. Struct. Dyn. 2, 327 (1985)). Unlike the well studied transitions to the cruciform and to the Z form, this novel transition was strongly pH-dependent. We have found the (dG)n · (dC)n insert to undergo a pH-dependent structural transition similar to that of the (GA)n · (TC)n tract. These new data meet our earlier expectations and disagree with the data of D.E. Pulleyblank, D.B. Haniford and A.R. Morgan, Cell 42, 271 (1985). We conclude that a novel DNA structure (the H-form) is typical of homopurine-homopyrimidine mirror repeats (the H palindromes) under superhelical stress and/or acid pH. In the H-form the homopyrimidine strand forms a hairpin while half of the homopurine strand interacts with the hairpin forming a triplex, the other half of the homopurine strand being unstructured (V.I. Lyamichev, S.M. Mirkin and M.D. Frank-Kamenetskii, J. Biomol. Struct. Dyn. 2, 3,667 (1986)). 相似文献
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