A New Approach to Potentiate Site-Specific Hybridization: A set of Hydrophobic Heterobifunctional Short Oligodeoxyribonucleotides

Abstract

An effective approach to enhance the short oligonucleotide reagent to attack ssDNA based on the use of a set of short oligonucleotide heterobifunctional derivatives bearing steroid residues is proposed.     

The ratio of systemic and coronary fractions of left ventricular output in reflex pressor reactions

In acute experiments the participation of coronary and systemic fractions was studied during suppression of the carotid sinus baroreceptors by the occlusion of the carotid arteries and stimulation of the tibial nerve afferent fibers. In most tests systemic fraction was reduced in carotid arteries occlusion and increased in tibial nerve stimulation. The coronary fraction was always increased. The cardiac output (the sum of systemic and coronary fractions) was steady with blood pressure increase by 20 to 70%. The role of coronary fraction in the mechanism of homeometric regulation of the heart is discussed.     

The effect of practolol and atenolol on the lysosomal enzyme activity of the ventricular myocardium of rats]

The influence of cardioselective beta-blockers, practolol and atenolol, on acid phosphatase, acid deoxyribonuclease, cathepsin D, beta-glucosidase and beta-galactosidase activities was studied in homogenates of intact rat ventricular myocardium. In the presence of drugs (1 x 10(-9)-1 x 10(-5) M) the activities of acid phosphatase, cathepsin D, beta-glucosidase and beta-galactosidase tended to diminish but the activity of acid deoxyribonuclease tended to increase. Some differences in the influence of drugs on the enzyme activities were removed by prolongation of preincubation of homogenates with drugs. It is supposed that the mechanism of influence of beta-blockers on lysosomes of the intact rat ventricular myocardium in conditions of this study includes the specific drug binding to beta-adrenergic receptors situated on lysosomes.     

Study of discharge quenching in the T-10 tokamak by injecting high-Z impurity pellets

The problem of the fast quenching of a discharge in tokamaks by injecting high-Z impurity pellets is considered. Results are presented from experiments in the T-10 tokamak, in which a substantial decrease (up to 70%) in the thermal plasma energy was observed. A one-dimensional transport code is developed to describe tokamak-discharge quenching. The code is used to simulate the experiments on quenching the T-10 discharge. It is shown that the injection of a high-Z pellet into the T-10 plasma changes the transport coefficients as compared to their steady-state values derived from the energy balance or scalings.