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231.
Behet Kemal alar Serdar Satar Assunta Bertaccini Toufic Elbeaino 《Journal of Phytopathology》2019,167(4):248-255
During 2017, maize cultivation areas in the provinces of Adana and Kahramanmara? (Turkey) were surveyed to inspect maize plants with symptoms similar to those associated with of phytoplasma disease, that is, yellowing, short internodes and small corncobs. Thirty fields were inspected and two hundred samples from symptomatic and asymptomatic plants were collected, together with insects considered as potential vectors of phytoplasmas. All samples were assayed by polymerase chain reaction (PCR) and subsequently analysed by restriction fragment length polymorphism and sequencing to identify the phytoplasmas detected in the plant material and insects. Results of laboratory assays and phylogenetic analyses showed that the Bermudagrass white leaf phytoplasma ('Candidatus Phytoplasma cynodontis') was present in both maize plants and seeds, showing 99% sequence identity with other reported phytoplasma strains from GenBank, whereas no PCR amplifications were obtained from tested insects. The seeds of infected plants, sown in an insect‐proof screenhouse, produced plantlets that were found PCR‐positive for the Bermudagrass white leaf phytoplasma, indicating its seed transmission. 相似文献
232.
Ahlam Al-Ghamdi Serdar Coskun Saad Al-Hassan Rafat Al-Rejjal Khalid Awartani 《Reproductive biology and endocrinology : RB&E》2008,6(1):37
Background
To evaluate the relationship between endometrial thickness on day of human chorionic gonadotrophin administration (hCG) and pregnancy outcome in a large number of consecutive in vitro fertilization and embryo transfer (IVF-ET) cycles. 相似文献233.
Korkmaz A Kurt B Yildirim I Basal S Topal T Sadir S Oter S 《Experimental biology and medicine (Maywood, N.J.)》2008,233(3):338-343
It was previously shown that nitric oxide produced by inducible nitric oxide synthase (iNOS) and peroxynitrite are responsible for cyclophosphamide (CP)-induced cystitis. Since endogenous production of peroxynitrite is known to lead to poly(ADP-ribose) polymerase (PARP) activation, in this study, the aim was to evaluate whether the PARP activation pathway is also included in the pathogenesis of CP-induced bladder ulceration in rats. A total of 48 male albino Wistar rats were divided into 5 groups. Group 1 served as control and was given 2 ml saline; four groups received a single dose of CP (200 mg/kg) with the same time intervals. Group 2 received CP only; Group 3, selective iNOS inhibitor 1400W (20 mg/kg); Group 4, peroxynitrite scavenger ebselen (30 mg/kg); and Group 5, PARP inhibitor 3-aminobenzamide (20 mg/kg). CP injection resulted in severe cystitis with continuous macroscopic hemorrhage, strong edema, inflammation, and ulceration. Moreover, bladder iNOS activation and urine nitrite-nitrate levels were dramatically increased. Histologically, 1400W protected bladder against CP damage and decreased urine nitrite-nitrate levels and bladder iNOS induction. Ebselen has shown similar histologic results with 1400W without changing urinary nitrite-nitrate level and iNOS activity. Furthermore in the 3-aminobenzamide group, beneficial effects had also occurred including decreased ulceration. These results suggest that PARP activation involves pathogenesis of CP-induced bladder ulceration. Furthermore, PARP is not only important for ulceration but also for bladder edema, hemorrhage, and inflammation because of broken uroepithelial cellular integrity. 相似文献
234.
Serap Gençer Öznur Ak Nur Benzonana Ayşe Batırel Serdar Özer 《Annals of clinical microbiology and antimicrobials》2002,1(1):2-4
Background
Pseudomonas aeruginosa is the third most common pathogen responsible for nosocomial infections and the prevalence of multiple resistant isolates has been increasing. Ninety-nine clinical isolates were studied in order to assess the current levels of susceptibility and cross-resistances of widely used antipseudomonal antibiotics against P. aeruginosa and to determine some resistance mechanisms by phenotypic methods. 相似文献235.
María Muñoz‐Amatriaín Pei Xu Steve I. Wanamaker MingCheng Luo Hind Alhakami Matthew Alpert Ibrahim Atokple Benoit J. Batieno Ousmane Boukar Serdar Bozdag Ndiaga Cisse Issa Drabo Jeffrey D. Ehlers Andrew Farmer Christian Fatokun Yong Q. Gu Yi‐Ning Guo Bao‐Lam Huynh Scott A. Jackson Francis Kusi Cynthia T. Lawley Mitchell R. Lucas Yaqin Ma Michael P. Timko Jiajie Wu Frank You Noelle A. Barkley Philip A. Roberts Stefano Lonardi Timothy J. Close 《The Plant journal : for cell and molecular biology》2017,89(5):1042-1054
Cowpea (Vigna unguiculata L. Walp.) is a legume crop that is resilient to hot and drought‐prone climates, and a primary source of protein in sub‐Saharan Africa and other parts of the developing world. However, genome resources for cowpea have lagged behind most other major crops. Here we describe foundational genome resources and their application to the analysis of germplasm currently in use in West African breeding programs. Resources developed from the African cultivar IT97K‐499‐35 include a whole‐genome shotgun (WGS) assembly, a bacterial artificial chromosome (BAC) physical map, and assembled sequences from 4355 BACs. These resources and WGS sequences of an additional 36 diverse cowpea accessions supported the development of a genotyping assay for 51 128 SNPs, which was then applied to five bi‐parental RIL populations to produce a consensus genetic map containing 37 372 SNPs. This genetic map enabled the anchoring of 100 Mb of WGS and 420 Mb of BAC sequences, an exploration of genetic diversity along each linkage group, and clarification of macrosynteny between cowpea and common bean. The SNP assay enabled a diversity analysis of materials from West African breeding programs. Two major subpopulations exist within those materials, one of which has significant parentage from South and East Africa and more diversity. There are genomic regions of high differentiation between subpopulations, one of which coincides with a cluster of nodulin genes. The new resources and knowledge help to define goals and accelerate the breeding of improved varieties to address food security issues related to limited‐input small‐holder farming and climate stress. 相似文献
236.
The superoxide scavenging activities of copper(II) complexes with the ligands, 6,6′-methylene-bis(5′-amino-3′,4′-benzo-2′-thiapentyl)-1,11-diamino-2,3:9,10-dibenzo-4,8-dithiaundecane (H4L), and 6,6′-bis(5′-amino-3′,4′-benzo-2′-thiapentyl)-1,11-diamino-2,3:9,10-dibenzo-4,8-dithiaundecane (H4L′), were investigated by xanthine–xanthine oxidase (X/XO) assays using nitroblue tetrazolium (NBT) as indicator molecule, and the results were compared with respect to the particular type of anion (ClO·4, Cl·, NO·3) on the apical site of the copper(II) complexes. All of the complexes inhibited the reduction of NBT by superoxide radicals, with the [Cu2(L′)](ClO4)2 complex exhibiting the highest scavenging activity against superoxide radicals among the complexes examined. The catalytic efficiency of the complexes for dismutation of superoxide radicals depends on the particular anion liganded to Cu(II) ion in the complexes, and the order of potency was observed to be ClO4 > Cl > NO·3 in phosphate buffer at pH 7.40. The Cu(II)-H4L′ complexes had the lowest IC50 and catalytic rate constant values indicating that the distorted geometry of the Cu(II)-H4L′ complexes influence their catalytic activities for dismutation of superoxide radicals more efficiently. The difference in the activities of the complexes toward superoxide radicals can also be attributed to the nature of the anions on the apical site of the copper(II) complexes and the superoxide dismutase-like activity. © 1997 John Wiley & Sons, Inc. J Biochem Toxicol 12: 53–59, 1998 相似文献
237.
Khalid A Awartani Samar Nahas Saad H Al Hassan Mashael A Al Deery Serdar Coskun 《Reproductive biology and endocrinology : RB&E》2009,7(1):52
Background
Obesity is a common disorder with a negative impact on IVF treatment outcome. It is not clear whether morbidly obese women (BMI >= 35 kg/m2) respond to treatment differently as compared to obese women (BMI = 30–34.9 kg/m2) in IVF. Our aim was to compare the outcome of IVF or ICSI treatments in obese patients to that in morbidly obese patients. 相似文献238.
Surasri N. Sahu Jada Lewis Isha Patel Serdar Bozdag Jeong H. Lee Robert Sprando Hediye Nese Cinar 《PloS one》2013,8(7)
Arsenic, a known human carcinogen, is widely distributed around the world and found in particularly high concentrations in certain regions including Southwestern US, Eastern Europe, India, China, Taiwan and Mexico. Chronic arsenic poisoning affects millions of people worldwide and is associated with increased risk of many diseases including arthrosclerosis, diabetes and cancer. In this study, we explored genome level global responses to high and low levels of arsenic exposure in Caenorhabditis elegans using Affymetrix expression microarrays. This experimental design allows us to do microarray analysis of dose-response relationships of global gene expression patterns. High dose (0.03%) exposure caused stronger global gene expression changes in comparison with low dose (0.003%) exposure, suggesting a positive dose-response correlation. Biological processes such as oxidative stress, and iron metabolism, which were previously reported to be involved in arsenic toxicity studies using cultured cells, experimental animals, and humans, were found to be affected in C. elegans. We performed genome-wide gene expression comparisons between our microarray data and publicly available C. elegans microarray datasets of cadmium, and sediment exposure samples of German rivers Rhine and Elbe. Bioinformatics analysis of arsenic-responsive regulatory networks were done using FastMEDUSA program. FastMEDUSA analysis identified cancer-related genes, particularly genes associated with leukemia, such as dnj-11, which encodes a protein orthologous to the mammalian ZRF1/MIDA1/MPP11/DNAJC2 family of ribosome-associated molecular chaperones. We analyzed the protective functions of several of the identified genes using RNAi. Our study indicates that C. elegans could be a substitute model to study the mechanism of metal toxicity using high-throughput expression data and bioinformatics tools such as FastMEDUSA. 相似文献
239.
Nilay Coplu Hüsniye Simsek Aysegul Gozalan Gülsen Hascelik Serpil Ercis Zeynep Senses Mehmet Baysallar Ipek I. Mumcu Neriman Balaban Serdar Terzioglu Ozkan Sengul Berrin Esen 《Annals of microbiology》2006,56(4):379-381
Co-trimoxazole resistance inEscherichia coli isolates from urinary tract infections (UTI) was assessed in 382 strains from 1995 and 510 strains from 2005. The strains were collected from five microbiology laboratories in Ankara, Turkey. Documentation on patient gender, age and outpatient/inpatient status was collected in 2005, but not in 1995. The resistance percentages were 751% in 1995 and 55.5% in 2005. This reduction in resistance percentage was statistically significant, overall in all except two of the participating laboratories. The resistance percentage in 2005 was 61.1% for children (n=208) and 51.2% for adults (n=258), 53.7% for females (n=380) and 60.8% for males (n=130), and 55.3% for outpatients (n=400) and 56.4% for inpatients (n=110). The reduction in resistance is believed to be a consequence of reduced usage. Although decreased, the level of co-trimoxazole resistance remains high, and continued avoidance of its use for empiric treatment of UTI in Turkey appears to be an appropriate strategy. 相似文献
240.