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101.
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The force fields commonly used in molecular dynamics simulations of proteins are optimized under bulk conditions. Whether the same force fields can be used in simulations of membrane proteins is not well established, although they are increasingly being used for such purposes. Here we consider ion permeation in the gramicidin A channel as a test of the AMBER force field in a membrane environment. The potentials of mean force for potassium ions are calculated along the channel axis and compared with the one deduced from the experimental conductance data. The calculated result indicates a rather large central barrier similar to those obtained from other force fields, which are incompatible with the conductance data. We suggest that lack of polarizability is the most likely cause of this problem, and, therefore, urge development of polarizable force fields for simulations of membrane proteins. 相似文献
103.
Koc A Narci A Duru M Gergerlioglu HS Akaydin Y Sogut S 《Molecular and cellular biochemistry》2005,280(1-2):193-199
Testicular torsion and detorsion are important clinical problems for infertile man and oxidative stress may have a role in
this clinical situation. The aim of this study was to investigate the protective role of erdosteine, an antioxidant, on unilateral
testicular reperfusion injury in rats. The rats were divided into four groups including seven rats in each group: control,
torsion, torsion/detorsion and torsion/detorsion+erdosteine. Rats, except the sham operation group, were subjected to left
unilateral torsion (720∘ rotation in the clockwise direction) without including the epididymis. The experiments were finished after sham operation
time for control, 120 min torsion for torsion group and 120 min torsion and 240 min detorsion for torsion/detorsion groups.
Bilateral orchiectomy was performed for all groups of rats. The ipsilateral and controlateral testis were divided into two
pieces to analyse biochemical parameters and to investigate the light microscopic view.
Malondialdehyde level of ipsilateral testis was increased in torsion and torsion/detorsion groups in comparison with the other
groups (p < 0.05). Erdosteine treatment ameliorated lipid peroxidation after torsion/detorsion in ipsilateral testis (p < 0.05). Also, xanthine oxidase activity of ipsilateral testis was increased in torsion/detorsion group in comparison with
the others (p < 0.05). Nitric oxide (NO) level of ipsilateral testis was higher in all experimental groups than sham operated control group
(p < 0.05). Also, NO level of torsion group was increased in comparison with detorsion groups (p < 0.05). Erdosteine treatment caused increased glutathione peroxidase activity in comparison with torsion and torsion/detorsion
groups and catalase activity in comparison with the other groups in ipsilateral testis (p < 0.05). Superoxide dismutase activity of ipsilateral testis was higher in torsion/detorsion and torsion/detorsion+erdosteine
groups than control and torsion groups (p < 0.05). The biochemical parameters were not affected in controlateral testis in all groups. Torsion, torsion/detorsion and
torsion/detorsion+erdosteine groups showed ipsilateral testicular damage in the histological examination, but the specimens
from torsion/detorsion had a significantly greater histological injury than those from the other groups (p < 0.05). Control rats showed normal seminiferous tubule morphology. Rats in torsion group had slight-to-moderate disruption
of the seminiferous epithelium. Rats in torsion/detorsion group displayed moderate-to-severe disruption of the seminiferous
epithelium. In all animals from torsion/detorsion+erdosteine group, the testicular tissues were affected with slight-to-moderate
degenerative changes of the seminiferous epithelium. Administration of erdosteine resulted in a significantly reduced histological
damage associated with torsion of the spermatic cord compared with torsion/detorsion. In all groups, the contralateral testes
were histologically normal.
In conclusion, the results clearly displayed that erdosteine treatment may have a protective role on testicular torsion/detorsion
injury. (Mol Cell Biochem xxx: 193–199, 2005) 相似文献
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Yumuk PF Gumus M Ekenel M Basaran G Aliustaoglu M Topal A Turhal NS 《Genetic testing》2004,8(2):169-173
To identify attitudes toward genetic testing, and the effects of this information on decisions regarding issues such as pregnancy, abortion, and prophylactic surgery, several subsets of the Turkish population were surveyed in hospital settings. Individuals (n = 179) chosen arbitrarily from four different subsets of a Turkish population were asked to participate in a confidential 23-question survey. Survey participants were familiar with the concept of cancer being a familial disease (85.5%), and 84.7% of them expressed interest in genetic testing to determine cancer risk, 83.9% would have their fetuses tested for such cancer risk, 65.1% would terminate their pregnancies, 92.2% would have their children tested if they were determined to have an increased cancer risk, 71.9% would agree to undergo prophylactic oophorectomy or orchiectomy and 67.6% would have mastectomy/prostatectomy should there be an increased cancer risk to these organs. It appears that at least the sampled segment of a Turkish population is willing to undergo genetic testing to determine if they are at increased risk for cancer. The feasibility and acceptance of genetic testing and the influence of education and genetic counseling in the Turkish people should further be evaluated with a larger stratified sample of the population. 相似文献
108.
The aim of this study was to investigate the molecular epidemiology of Stenotrophomonas maltophilia in a university hospital in Turkey. Thirty nine clinical isolates were collected from 37 patients and one from an environmental source between 1998 and 2001. Susceptibility to 11 antimicrobials was studied. The isolates were categorised into six groups: A through F. Trimethoprim sulfamethoxazole was the most active agent against the tested isolates. Genotypic analysis by pulsed-field gel electrophoresis (PFGE) of clinical isolates identified 21 different PFGE patterns. Three most common clusters were composed of 11, seven and four strains. Antimicrobial susceptibility identified multi-resistant phenotype in all S. maltophilia PFGE clones. All the remaining 18 isolates (45%) revealed unique PFGE patterns. Resistance was not lower in unique strains. The clones mainly with two unique macrorestriction profiles strongly suggests nosocomial transmission of these strains from either a common source and/or between patients. 相似文献
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Marta Romani Francesca Mancini Alessia Micalizzi Andrea Poretti Elide Miccinilli Patrizia Accorsi Emanuela Avola Enrico Bertini Renato Borgatti Romina Romaniello Serdar Ceylaner Giangennaro Coppola Stefano D’Arrigo Lucio Giordano Andreas R. Janecke Mario Lituania Kathrin Ludwig Loreto Martorell Tommaso Mazza Sylvie Odent Lorenzo Pinelli Pilar Poo Margherita Santucci Sabrina Signorini Alessandro Simonati Ronen Spiegel Franco Stanzial Maja Steinlin Brahim Tabarki Nicole I. Wolf Federica Zibordi Eugen Boltshauser Enza Maria Valente 《Human genetics》2015,134(1):123-126