Cyanidin-3-glucoside, a natural product derived from blackberry, exhibits chemopreventive and chemotherapeutic activity

Epidemiological data suggest that consumption of fruits and vegetables has been associated with a lower incidence of cancer. Cyanidin-3-glucoside (C3G), a compound found in blackberry and other food products, was shown to possess chemopreventive and chemotherapeutic activity in the present study. In cultured JB6 cells, C3G was able to scavenge ultraviolet B-induced *OH and O2-* radicals. In vivo studies indicated that C3G treatment decreased the number of non-malignant and malignant skin tumors per mouse induced by 12-O-tetradecanolyphorbol-13-acetate (TPA) in 7,12-dimethylbenz[a]anthracene-initiated mouse skin. Pretreatment of JB6 cells with C3G inhibited UVB- and TPA-induced transactivation of NF-kappaB and AP-1 and expression of cyclooxygenase-2 and tumor necrosis factor-alpha. These inhibitory effects appear to be mediated through the inhibition of MAPK activity. C3G also blocked TPA-induced neoplastic transformation in JB6 cells. In addition, C3G inhibited proliferation of a human lung carcinoma cell line, A549. Animal studies showed that C3G reduced the size of A549 tumor xenograft growth and significantly inhibited metastasis in nude mice. Mechanistic studies indicated that C3G inhibited migration and invasion of A549 tumor cells. These finding demonstrate for the first time that a purified compound of anthocyanin inhibits tumor promoter-induced carcinogenesis and tumor metastasis in vivo.     

乌龟血液生物化学指标的初步研究

乌龟Chinemys reevesii 隶属龟鳖目、龟科、乌龟属。       

Separation of human erythrocyte membrane associated proteins with one-dimensional and two-dimensional gel electrophoresis followed by identification with matrix-assisted laser desorption/ionization-time of flight mass spectrometry

A classical proteomic analysis was used to establish a reference map of proteins associated with healthy human erythrocyte ghosts. Following osmotic lysis and differential centrifugation, ghost proteins were separated by either one-dimensional gel electrophoresis (1-DE) or two-dimensional gel electrophoresis (2-DE). Selected protein bands or spots were excised and trypsinized before mass spectrometric analyses and data mining was performed using the SWISS-PROT and NCBI nonredundant databases. A total of 102 protein spots from a 2-D gel were successfully identified. These corresponded to 59 distinct polypeptides with the remaining 43 being isoforms. As for the 1-D gel, 44 polypeptides were identified, of which 19 were also found on the 2-D gel. Most of the 19 common polypeptides were membrane cytoskeletal proteins that are often referred to as the "band" proteins. The remaining 25 polypeptides that were found exclusively on 1-D gels were proteins with high hydrophobicity (e.g., sorbitol dehydrogenase and glucose transporter) and high molecular mass (e.g., Kell blood group glycoprotein and Janus-kinase 2). A higher number of signaling proteins was also identified on 1-D gels compared to 2-D gels. These included Ras, cAMP dependent protein kinase and TGF-beta receptor type 1 precursor.     

苋科植物化学成分的研究进展

苋科植物有65个属,约1000余种,主要分布在热带、亚热带和温带地区.该科的很多植物具有良好的食用和药用价值,化学成分丰富,含有三萜类、甾体类、黄酮类、生物碱类、色素类和多肽类物质.为推动我国苋科植物资源的更深层次利用和开发,对苋科植物的化学成分研究概况做一综述.     

墨兰的无菌播种和植株再生

墨兰的无菌播种结果发现,在不添加细胞分裂素的培养基上,种子可以发芽,但只有原球茎和根状茎产生;不可能进一步分化成苗,只有在含有不同激素成分的MS或KnudsonC培养基上,才有可能诱导芽的分化,其中以附加6-BA 0.5-1.0mg/L+NAA 0.1mg/L诱导效果最佳,在附加6-BA 2.0mg/L+NAA 0.4mg/L的MS培养基中,能加速芽的增殖,根状茎转入含有相同激素成分的液体增殖培养基中振荡培养,可大大提高芽的分化速率。添加0.5%活性炭对芽的分化有明显增效作用。在附加NAA 0.2mg/L的MS培养基中;幼苗的生根效果最佳。     

Environmental Supply Chain Cooperation and Its Effect on the Circular Economy Practice‐Performance Relationship Among Chinese Manufacturers

Due to resource scarcity and environmental degradation, a new development concept emphasizing environmental concerns, called the circular economy (CE), has been enacted in legislation in China. This environmental management concept can be implemented at three levels, namely, region, industrial zone, and individual enterprise, with the objective of boosting economic development while lessening environmental and resource challenges. Environmental supply chain cooperation (ESCC), an approach that utilizes customer and supplier cooperation in environmental management, has been initiated among Chinese enterprises. Using survey data collected from 396 Chinese manufacturers, we examine the role of ESCC practices in influencing the relationship between implementing CE practices and the achievement of performance outcomes by testing the moderation and mediation effects of ESCC practices on the CE practice‐performance relationship through hierarchical regression analysis. Our data analyses indicate that ESCC practices are useful by moderation and, in some cases, essential by mediation, for Chinese manufacturers seeking to realize the performance targets desired in CE practices. The results highlight the need for Chinese manufacturers to improve supply chain coordination in their implementation of CE. On the policy side, our research findings suggest that ESCC practices are beneficial and, in some cases, necessary for the development of CE in China.     

分离自海洋沉积物的四个中国真菌新记录种

报道了分离自渤海沉积物的4个中国真菌新记录种：黄褐隐囊菌Aphanoascus fulvescens、小翅孢壳Emericellopsis minima、弗氏光黑壳Preussia flanaganii和湿生假散囊菌Pseudeurotium hygrophila。其中,隐囊菌属Aphanoascus为中国新记录属。研究菌株保存于中国海洋大学海洋生物标本室（OUCMB）。     

果蝇求偶行为分子调控机制的研究进展

果蝇(Drosophila)的求偶行为受多个基因调控,例如fruitless(fru)、dissatisfaction(dsf)和retained(retn)等。它们通过不同的剪切方式产生特异性产物,利用这些产物来控制雌雄果蝇的求偶行为,它们的剪切方式是雌雄果蝇求偶行为和性别决定所必需的。主要阐述了这些基因在果蝇求偶行为方面的分子调控机制,为进一步研究果蝇的求偶行为和性别决定提供理论依据。     

母子分离应激对complexin Ⅱ基因缺陷鼠快速移动行为的影响

本文旨在探讨母子分离应激对complexin Ⅱ基因缺陷(Cplx2-/-)小鼠快速移动行为的影响。实验分4组:应激Cplx2-/-组、应激Cplx2+/+组、无应激Cplx2-/-组和无应激Cplx2+/+组。无应激组小鼠正常饲养,应激组小鼠从出生后第2日至第21日每天施加母子分离应激。小鼠生长到第4周时,利用聚合酶链反应技术检测其基因型。4组小鼠腹腔注射激动剂去氧麻黄碱或生理盐水后,采用EthoVision系统对小鼠快速移动行为进行记录。结果显示,应激Cplx2-/-组、应激Cplx2+/+组、无应激Cplx2-/-组和无应激Cplx2+/+组注射去氧麻黄碱后,与生理盐水对照组相比,均出现快速移动行为不同程度的增多(P<0.01);与无应激Cplx2-/-组相比较,应激Cplx2-/-组注射去氧麻黄碱后快速移动行为增多更为明显(P<0.001);与应激Cplx2+/+组相比,应激Cplx2-/-组注射去氧麻黄碱后的快速移动行为增多更为明显(P<0.001)。本实验证实小鼠的行为可能受母子分离应激和Cplx2基因等因素的多重影响。     

1α羟化酶活性和血钙水平对24羟化酶基因表达的影响

目的:研究肾脏24羟化酶基因表达的影响因素。方法:采用两种基因敲除小鼠。每种小鼠又分两种饲养方式。用生化分析仪测定小鼠血钙浓度。用半定量RT-PCR法研究小鼠肾脏组织中1α羟化酶和24羟化酶基因的表达。结果:1α羟化酶基因敲除小鼠体内血钙低于野生型小鼠(78±10.4 mg/Lvs111±16.5 mg/L,P<0.05.),测不出24羟化酶基因表达。维生素D受体基因敲除小鼠有很高的1α羟化酶表达,小鼠血钙也显著低于野生型小鼠(68±9.8 mg/Lvs111±16.5 mg/L,P<0.05),测不出24羟化酶表达。但给予高乳糖饲料后,两种基因敲除小鼠血钙都上升到与野生型小鼠一致水平。此时,24羟化酶基因的表达与野生型也基本一致。结论:血钙是调节24羟化酶基因表达的直接因素,1α羟化酶对24羟化酶的正向调节作用是通过升高血钙来实现的。