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121.
122.
Chang-Kug Kim Young-Joo Seol Dong-Jun Lee Jae-Hee Lee Tae-Ho Lee Dong-Suk Park 《Bioinformation》2014,10(10):664-666
The National Agricultural Biotechnology Information Center (NABIC) in South Korea reconstructed a RiceQTLPro database for
gene positional analysis and structure prediction of the chromosomes. This database is an integrated web-based system providing
information about quantitative trait loci (QTL) markers in rice plant. The RiceQTLPro has the three main features namely, (1) QTL
markers list, (2) searching of markers using keyword, and (3) searching of marker position on the rice chromosomes. This updated
database provides 112 QTL markers information with 817 polymorphic markers on each of the 12 chromosomes in rice.
Availability
The database is available for free at http://nabic.rda.go.kr/gere/rice/geneticMap/ 相似文献123.
Daud?Kassamkassam@cc.kochi-u.ac.jp; KY yamaoka@cc.kochi-u.ac.jp" title="DK kassam@cc.kochi-u.ac.jp; KY yamaoka@cc.kochi-u.ac.jp" itemprop="email" data-track="click" data-track-action="Email author" data-track-label="">Email author Shinji?Mizoiri Kosaku?Yamaoka 《Ichthyological Research》2004,51(3):195-201
Differences in color patterns have been the most used feature in describing cichlid species belonging to genus Petrotilapia from Lake Malawi. In this study, we quantified morphological variation in body shape within and among three coexisting Petrotilapia species using landmark-based geometric morphometric methods. Statistic analyses revealed significant body shape differences among species but not between sexes. Post hoc multiple comparisons based on Mahalanobis distances revealed that P. nigra was significantly different from P. genalutea and Petrotilapia sp., whereas the latter two were not significantly different. The splines generated showed that the most pronounced variation was in the head region, in which P. nigra had a relatively longer and deeper head than the other two. The most clear-cut distinction was in gape length; P. genalutea had the longest gape, followed by Petrotilapia sp., whereas P. nigra had the shortest gape. Body depth was shallower in P. nigra than the others. When comparing sexes by their centroid size, ANOVA revealed that males were bigger than females. Therefore, we conclude that color is not the only feature that can distinguish these congeners. We discuss the observed sexual dimorphism in terms of sexual selection and relate morphological variation among species to feeding behavior, which may help explain their coexistence in nature. 相似文献
124.
125.
Yu HS Jeong HJ Hong YC Seol SY Chung DI Kong HH 《The Korean journal of parasitology》2007,45(1):11-18
Recent in vitro studies have revealed that a certain Mycobacterium can survive and multiply within free-living amoebae. It is believed that protozoans function as host cells for the intracellular replication and evasion of Mycobacterium spp. under harmful conditions. In this study, we describe the isolation and characterization of a bacterium naturally observed within an amoeba isolate acquired from a contact lens storage case. The bacterium multiplied within Acanthamoeba, but exerted no cytopathic effects on the amoeba during a 6-year amoebic culture. Transmission electron microscopy showed that the bacteria were randomly distributed within the cytoplasm of trophozoites and cysts of Acanthamoeba. On the basis of the results of 18S rRNA gene analysis, the amoeba was identified as A. lugdunensis. A 16S rRNA gene analysis placed this bacterium within the genus Mycobacterium. The bacterium evidenced positive reactivity for acid-fast and fluorescent acid-fast stains. The bacterium was capable of growth on the Middlebrook 7H11-Mycobacterium-specific agar. The identification and characterization of bacterial endosymbionts of free-living protozoa bears significant implications for our understanding of the ecology and the identification of other atypical mycobacterial pathogens. 相似文献
126.
Luciano G Martelotto Charlotte KY Ng Maria R De Filippo Yan Zhang Salvatore Piscuoglio Raymond S Lim Ronglai Shen Larry Norton Jorge S Reis-Filho Britta Weigelt 《Genome biology》2014,15(10)
Background
Massively parallel sequencing studies have led to the identification of a large number of mutations present in a minority of cancers of a given site. Hence, methods to identify the likely pathogenic mutations that are worth exploring experimentally and clinically are required. We sought to compare the performance of 15 mutation effect prediction algorithms and their agreement. As a hypothesis-generating aim, we sought to define whether combinations of prediction algorithms would improve the functional effect predictions of specific mutations.Results
Literature and database mining of single nucleotide variants (SNVs) affecting 15 cancer genes was performed to identify mutations supported by functional evidence or hereditary disease association to be classified either as non-neutral (n = 849) or neutral (n = 140) with respect to their impact on protein function. These SNVs were employed to test the performance of 15 mutation effect prediction algorithms. The accuracy of the prediction algorithms varies considerably. Although all algorithms perform consistently well in terms of positive predictive value, their negative predictive value varies substantially. Cancer-specific mutation effect predictors display no-to-almost perfect agreement in their predictions of these SNVs, whereas the non-cancer-specific predictors showed no-to-moderate agreement. Combinations of predictors modestly improve accuracy and significantly improve negative predictive values.Conclusions
The information provided by mutation effect predictors is not equivalent. No algorithm is able to predict sufficiently accurately SNVs that should be taken forward for experimental or clinical testing. Combining algorithms aggregates orthogonal information and may result in improvements in the negative predictive value of mutation effect predictions.Electronic supplementary material
The online version of this article (doi:10.1186/s13059-014-0484-1) contains supplementary material, which is available to authorized users. 相似文献127.
128.
Kim SM Lee HW Choi YW Kim SH Lee JC Lee YC Seol SY Cho DT Kim J 《Journal of microbiology (Seoul, Korea)》2012,50(1):175-178
In this study, we examined the biofilm forming ability, the mRNA expression of curli genes and the morphologies of curli fimbriae
and biofilms in clinical isolates of Enterobacter cloacae. The csgBA operon was found in 11 (78.6%) of the 14 isolates. The ability of E. cloacae isolates to form biofilms was significantly correlated with the mRNA expression level of the csgA and csgD genes. The curli protein fimbriae appeared as tangled fibers and the curli-proficient strain formed mature biofilms. Our
data suggest that the expression of the curli fimbriae play an important role in biofilm formation in E. cloacae. 相似文献
129.
Pasaje CF Bae JS Park BL Kim JH Cheong HS Uh ST Park CS Shin HD 《Molecular biology reports》2012,39(5):5913-5919
Modest effects of genes in various pathways are significant in the etiology of complex human diseases, including aspirin exacerbated
respiratory disease (AERD). By functioning as a relevant component of respiratory processes, the human kinesin family member C1 (KIFC1) is hypothesized to play a role in AERD pathogenesis. A case–control analysis was carried out by comparing the genotype distribution
of six KIFC1 single-nucleotide polymorphisms between 93 AERD cases and 96 aspirin-tolerant asthma controls in a Korean population. After
controlling for confounds, logistic and regression models via various modes of genetic inheritance facilitated the association
analysis. Initial results revealed significant association at 0.05 level of significance between several KIFC1 variations and AERD (P = 0.01–0.05, OR = 1.81–1.90) as well as fall rate of forced expiratory volume in the 1st second, an important diagnostic
marker of airways constriction (P = 0.04–0.05). However, the signals were not deemed significant after multiple testing corrections (P
corr > 0.05). Although the results do not support a major role of KIFC1 in AERD pathogenesis in a Korean asthma cohort, further replication and validation studies are required to clarify the current
findings. 相似文献
130.