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91.
92.
We outline a general strategy for determining the effective coarse-grained interactions between the amino acids of a protein from the experimentally derived native-state structures. The method is, in principle, free from any adjustable or empirically determined parameters, and it is tested on simple models and compared with other existing approaches. Proteins 30:244–248, 1998. © 1998 Wiley-Liss, Inc. 相似文献
93.
Haidong Tan Yueguang Li Ling Chen Takayuki Kudoh Tomonari Kasai Masaharu Seno 《Molecular Biology》2012,46(1):142-148
Green fluorescent protein (GFPuv) has been widely used as a reporter fused to individual targeting sequences. However, its
state in liquid and its effect on other proteins are still unclear. The conformational polymorphisms of glutathione-S-transferase-green
fluorescent protein (GST-GFPuv), GFPuv and GST were analyzed by native polyacrylamide gel, indicating that GST was in many
different states while GFPuv and GST-GFPuv were only in four and two slightly different states. Four different circular dichroism
spectra were obtained from the GFPuv polymorphisms. The single molecular behavior of GST-GFPuv and GFPuv was also characterized
by MALDI-TOF MS. Thus, we demonstrated that: (1) there might be four different structural polymorphisms for the native GFPuv;
(2) GFPuv could reduce its partner’s polymorphism as a fusion protein. Although GFPuv had many merits as a reporter, its unreliability
was found in the study. 相似文献
94.
Ishikawa Junko Fujimura Shigeto Kondo Motohiko Murai-Hatano Mari Goto Akitoshi Shinano Takuro 《Plant and Soil》2018,424(1-2):503-524
Plant and Soil - In the Mediterranean basin, reduction in cloudiness owing to climate change is expected to enhance solar ultraviolet (UV) levels and to decrease rainfall over the coming years,... 相似文献
95.
Summary A new immunohistochemical method for light and electron microscopy of tissue- and cell-specific antigens by using ferric colloid-labeled antibody is presented. The antibodies labeled with the cationic cacodylate ferric colloid are stable and bind specifically to the target antigens to show clearly the site of antigens in tissue sections and on free cells by Prussian blue reaction for light microscopy and by the specific figure of electron opaque ferric colloid particles for electron microscopy. The staining procedure is very simple and it gives clear picture. So the method will be of beneficial for general laboratory use in immuno-histochemical researches. 相似文献
96.
Based on statistical variance as an index of electroencephalogram (EEG) parameters, we monitored slow-wave sleep in both humans and rats in real time and on-line with a widely used personal computer. This EEG variance method may be a useful tool to carry out biological rhythm research, including sleep studies. 相似文献
97.
Dai Ayusawa Kimiko Iwata Takeshi Seno 《Biochemical and biophysical research communications》1980,96(4):1654-1661
Of 625 aphidicolin-resistant clones selected at 33.5°C from mutagenized mouse FM3A cells, 13 clones could not grow at 39.5°C. Five of these clones, chosen at random, resumed growth at 39.5°C when thymidine was added to the culture medium. In hybrids, conditional thymidine auxotrophy was a recessive trait, but aphidicolin-resistance was either a codominant or recessive one depending on the mutant clone used.Thymidylate synthetase activity in crude extracts of these mutants was completely inactivated by preincubation for 30 min at 42°C, whereas that of the parent cells was not affected by the same treatment. Thus, the temperature-sensitive growth of the mutants described here seems to be due to this heat-sensitive thymidylate synthetase. 相似文献
98.
Masanori A. Murayama Shigeru Kakuta Takumi Maruhashi Kenji Shimizu Akimasa Seno Sachiko Kubo Nozomi Sato Shinobu Saijo Masahira Hattori Yoichiro Iwakura 《Biochemical and biophysical research communications》2014
Rheumatoid arthritis (RA) is an autoimmune inflammatory disease exhibited most commonly in joints. We found that the expression of C1qtnf3, which encodes C1q/TNF-related protein 3 (CTRP3), was highly increased in two mouse RA models with different etiology. To elucidate the pathogenic roles of CTRP3 in the development of arthritis, we generated C1qtnf3−/− mice and examined the development of collagen-induced arthritis in these mice. We found that the incidence and severity score was higher in C1qtnf3−/− mice compared with wild-type (WT) mice. Histopathology of the joints was also more severe in C1qtnf3−/− mice. The levels of antibodies against type II collagen and pro-inflammatory cytokine mRNAs in C1qtnf3−/− mice were higher than WT mice. These observations indicate that CTRP3 plays an important role in the development of autoimmune arthritis, suggesting CTRP3 as a possible medicine to treat RA. 相似文献
99.
Tsujii T. Akita M. Katayama K. Yamamoto S. Seno S. 《Histochemistry and cell biology》1984,81(5):427-433
Summary In order to reveal the absorption process of elastase from the intestine, hog pancreatic elastase was injected into the ligated jejunum lumen of the rat, and the tissues were cytochemically observed at various times after injection. The peroxidase anti-peroxidase (PAP) method using anti-hog-elastase rabbit antibody was used for light microscopy, and the anti-elastase Fab-peroxidase conjugate was used for electron microscopy. The tissues stained by the PAP method exhibited a dense deposition of reaction products on the luminal surface of epithelial cells and a moderate deposition in the blood and lymph capillaries of the intestinal villi. Immunoelectron microscopy revealed that the reaction product was deposited on the surface of the microvilli and in their pocketing; some was found in the pinocytotic vesicles in the terminal-web area and on the inner surface of the enlarged smooth endoplasmic reticulum. Round droplets which gave a positive reaction were found in the widened intercellular cleft and the thick basement membrane lining the blood capillaries and lymphatics. The jejunum retained its normal ultrastructure. The results indicate that the elastase molecules, which were introduced into the rat jejunum lumen, were absorbed without being decomposed through healthy intestinal epithelial cells by pinocytosis and translocated into blood and lymph capillaries. 相似文献
100.
Hideaki Matsushita Masanori Hijioka Akinori Hisatsune Yoichiro Isohama Shigeto Iwamoto Hiroaki Terasawa Hiroshi Katsuki 《PloS one》2013,8(7)
Intracerebral hemorrhage (ICH) is featured by poor prognosis such as high mortality rate and severe neurological dysfunction. In humans, several valuables including hematoma volume and ventricular expansion of hemorrhage are known to correlate with the extent of mortality and neurological dysfunction. However, relationship between hematoma conditions and the severity of symptoms in animal ICH models has not been clarified. Here we addressed this issue by using 7-tesla magnetic resonance imaging (MRI) on collagenase-induced ICH model in mice. We found that the mortality rate and the performance in behavioral tests did not correlate well with the volume of hematoma. In contrast, when hemorrhage invaded the internal capsule, mice exhibited high mortality and showed poor sensorimotor performance. High mortality rate and poor performance in behavioral tests were also observed when hemorrhage invaded the lateral ventricle, although worsened symptoms associated with ventricular hemorrhage were apparent only during early phase of the disease. These results clearly indicate that invasion of the internal capsule or the lateral ventricle by hematoma is a critical determinant of poor prognosis in experimental ICH model in mice as well as in human ICH patients. MRI assessment may be a powerful tool to refine investigations of pathogenic mechanisms and evaluations of drug effects in animal models of ICH. 相似文献