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21.
Daisuke Sawada Tomoyuki Katayama Yuya Tsukuda Nozomi Saito Masashi Takano Hiroshi Saito Ken-ichiro Takagi Eiji Ochiai Seiichi Ishizuka Kazuya Takenouchi Atsushi Kittaka 《Bioorganic & medicinal chemistry letters》2009,19(18):5397-5400
We synthesized and isolated 2α-substituted analogs of 14-epi-previtamin D3 after thermal isomerization at 80 °C for the first time. The VDR binding affinity and transactivation activity of osteocalcin promoter in HOS cells were evaluated, and the 2α-methyl-substituted analog was found to have greater genomic activity than 14-epi-previtamin D3. 相似文献
22.
Kana Tsukuda Masaki Mogi Jun Iwanami Li-Juan Min Fei Jing Kousei Oshima Masatsugu Horiuchi 《Biochemical and biophysical research communications》2011,(2):275
Angiotensin II type 1 (AT1) receptor blockers (ARBs) are known to prevent the onset of stroke and to attenuate neural damage. Additional beneficial effects of ARBs, independent of AT1 receptor blockade, have been highlighted. Irbesartan is reported to act as an antagonist of the monocyte chemoattractant protein-1 (MCP-1) receptor, C–C chemokine receptor 2 (CCR2), due to its molecular structure. We examined the possible synergistic effects of co-administration of irbesartan with propagermanium, a CCR2 antagonist, on ischemic brain damage. Administration of propagermanium decreased ischemic brain area after middle cerebral artery occlusion (MCAO). To study the possible synergistic effects of propagermanium with ARBs, we employed non-effective lower doses of irbesartan and losartan. Administration of irbesartan with propagermanium decreased the ischemic brain area more markedly compared with propagermanium alone, but co-administration of losartan did not. MCP-1 mRNA level was significantly increased on the ipsilateral side after MCAO, and administration of irbesartan with propagermanium decreased the MCP-1 level, whereas co-administration of losartan did not. Similar results were obtained for MCP-1 protein level. CCR2 mRNA expression was significantly elevated on the ipsilateral side; however, no significant difference was observed between each group. mRNA levels of other inflammatory cytokines such as TNF-α and IL-1β also significantly increased on the ipsilateral side, but the expression levels were not changed by each drug treatment. Taking these findings together, irbesartan exerts more beneficial effects on ischemic brain damage with an MCP-1 receptor blocker, at least due to its inhibitory effects on MCP-1/CCR2 signaling beyond AT1 receptor blockade. 相似文献
23.
Agnese Mancini Jesse Senko Ricardo Borquez-Reyes Juan Guzman Póo Jeffrey A. Seminoff Volker Koch 《Human ecology: an interdisciplinary journal》2011,39(6):743-756
Despite complete legal protection, improvements in infrastructure, and market conditions that provide easier access to other
protein sources, illegal poaching of sea turtles for consumption in Baja California Sur (BCS), Mexico remains a major threat
to their recovery. Few studies have focused on understanding the economic and social drivers behind this activity, which is
fundamental to determining best practices for discouraging it. From June 2007 to April 2008 we conducted eight in-depth, semi-structured
interviews with sea turtle poachers at five coastal communities in BCS to determine the drivers influencing them. The most
prevalent reasons for illegal poaching were direct economic benefits, lack of law enforcement and ease of escape from or bribery
of authorities, and strong family tradition. Our results suggest that to reduce illegal poaching it will be necessary to better
enforce existing environmental laws, reduce social acceptance of sea turtle hunting throughout the region, educate fishers
on the ecological importance of sea turtles, and show fishers direct economic benefits from non-consumptive use of sea turtles,
such as ecotourism. 相似文献
24.
Manabu Kaneko Koichi Watashi Shinji Kamisuki Hiroki Matsunaga Masashi Iwamoto Fumihiro Kawai Hirofumi Ohashi Senko Tsukuda Satomi Shimura Ryosuke Suzuki Hideki Aizaki Masaya Sugiyama Sam-Yong Park Takayoshi Ito Naoko Ohtani Fumio Sugawara Yasuhito Tanaka Masashi Mizokami Camille Sureau Takaji Wakita 《Journal of virology》2015,89(23):11945-11953
25.
Cloning, sequence analysis, and expression of ligninolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus 总被引:18,自引:0,他引:18
Y Kojima Y Tsukuda Y Kawai A Tsukamoto J Sugiura M Sakaino Y Kita 《The Journal of biological chemistry》1990,265(25):15224-15230
Two cDNAs and two genomic DNAs coding for the allelic forms of the ligninolytic phenoloxidase were isolated from the white-rot fungus Coriolus hirsutus. The cloned genes were identified in genetic libraries by hybridization screening using four deoxyoligonucleotide probes which corresponded to the partial amino acid sequence of the purified enzyme. Each cDNA encoded the full-length of the phenoloxidase, a protein consisting of 499 amino acid residues, and its putative signal peptide of 21 amino acid residues. The nucleotide sequences of the two alleles differed by 18 single base changes within the open reading frames resulting in one amino acid substitution. Ten small introns interrupted both genomic DNAs as indicated by direct comparison with the corresponding cDNAs. Putative eukaryotic regulatory sequences, "CAAT" and "TATA," were observed in the 5'-flanking region of both genomic DNAs. Each of the phenoloxidase cDNAs was successfully expressed in an active form in Saccharomyces cerevisiae using the useful yeast expression vector YEp51. 相似文献
26.
H Katagiri T Asano H Ishihara K Tsukuda J L Lin K Inukai M Kikuchi Y Yazaki Y Oka 《The Journal of biological chemistry》1992,267(31):22550-22555
The intracellular C-terminal domain is diverse in size and amino acid sequence among facilitative glucose transporter isoforms. The characteristics of glucose transport are also divergent, and GLUT2 has far higher Km and Vmax values compared with GLUT1. To investigate the role of the intracellular C-terminal domain in glucose transport, we expressed in Chinese hamster ovary cells the mutated GLUT1 protein whose intracellular C-terminal domain was replaced with that of GLUT2 by means of engineering the chimeric cDNA. Cytochalasin B, for which GLUT2 protein has much lower affinity, bound to this chimeric protein in a fashion similar to GLUT1. In contrast, greater transport activity was observed in this chimeric glucose transporter compared with the wild-type GLUT1 at 10 mM 2-deoxy-D-glucose concentration. The kinetic studies on 2-deoxy-D-glucose uptake revealed a 3.8-fold increase in Km and a 4.3-fold increase in Vmax in this chimeric glucose transporter compared with the wild-type GLUT1. Thus, replacement of the intracellular C-terminal domain confers the GLUT2-like property on the glucose transporter. These results strongly suggest that the diversity of intracellular C-terminal domain contributes to the diversity of glucose transport characteristics among isoforms. 相似文献
27.
T Asano H Katagiri K Tsukuda J L Lin H Ishihara K Inukai Y Yazaki Y Oka 《FEBS letters》1992,298(2-3):129-132
Digestion of human GLUT1 protein in erythrocytes with 5 micrograms/ml papain for 5 min yielded several fragments. By using several site-specific antibodies, two of these fragments containing the intracellular loop domain between M6 and M7 were demonstrated to be further digested by a prolonged incubation with papain. The addition of 0.2 M D-glucose enhanced this digestion between M6 and M7 by approximately 3.5-fold, while the addition of 0.2 M D-sorbitol exhibited no effects. These results strongly suggest that D-glucose binding induces the conformational change of the intracellular loop domain between M6 and M7 of GLUT1 protein. Since the homology of the amino acid sequence was low in this intracellular domain among the five facilitative glucose transporter isoforms, this intracellular loop might contribute to the difference in their Km and Vmax values for glucose uptake. 相似文献
28.
29.
Walter P. Cullen Jon Bordner Liang H. Huang Peter M. Moshier John R. Oscarson Laura A. Presseau Richard S. Ware Earl B. Whipple Yasuhiro Kojima Hiroshi Maeda Satoshi Nishiyama Junsuke Tone Katsuhitsu Tsukuda Kelvin S. Holdom John C. Ruddock 《Journal of industrial microbiology & biotechnology》1990,5(6):365-374
Summary CP-60,993, 19-epi-dianemycin, is a novel polycyclic ether antibiotic produced byStreptomyces hygroscopicus ATCC 39305. Fermentation recovery, purification and crystallization were achieved using standard procedures. CP-60,993 was characterized as a monocarboxylic acid. Elemental analysis suggested a molecular formula of C47H78O14 for the free acid and C47H77O14 Na for the sodium salt. Crystalline form CP-60,993 sodium salt shows the following properties: m.p. 193205°C, E
1 cm
1%
=157 at 232 nm, []
D
25°C
+11.0 (c 1, methanol). The structure, determined by MS, PMR and CMR, differs from dianemycin only in the stereochemistry at position 19. This was confirmed by X-ray crystallography carried out on the rubidium salt of CP-60,993. It exhibited activity in vitro against Gram-positive and anaerobic bacteria, efficacy againstEimeria coccidia in vivo in poultry, and stimulation in vitro of rumen propionic acid production. 相似文献
30.
The glucose transport activity of GLUT1 is markedly decreased by substitution of a single amino acid with a different charge at residue 415 总被引:1,自引:0,他引:1
H Ishihara T Asano H Katagiri J L Lin K Tsukuda Y Shibasaki Y Yazaki Y Oka 《Biochemical and biophysical research communications》1991,176(2):922-930
GLUT1 glucose transporter cDNA was modified to introduce a single amino acid substitution of aspartic acid for asparagine 415, which is conserved among all facilitative glucose transporter isoforms. Although a significant amount of the mutated transporter was expressed into plasma membranes of Chinese hamster ovary cells by transfection with expression vector, almost no increase in glucose transport activity was observed. Analysis of glucose uptake with Lineweaver-Burk plot depicts that the mutation induced a marked decrease (more than 5-fold) in turnover number and a slight increase (1.5-fold) in Km compared with the wild-type GLUT1. Results obtained with cytochalasin B and ethylidene glucose suggested that the inner but not outer glucose binding site was modulated. These results suggest that asparagine 415 is located close to the inner glucose binding site and the putative inner gate of GLUT1 glucose transporter and that an ionic charge in this domain might play an important role in the rate of conformational change between an inward-facing form and an outward-facing form of glucose transporter. 相似文献