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221.
B Merola A Colao M Cataldi E Rossi A Selleri F Briganti G Schettini A Bellastella G Lombardi 《Hormone research》1992,37(1-2):18-22
In some acromegalics, GH release can be induced by TRH and/or LHRH administration. The pathogenesis of these GH paradoxical responses was supposed to be a somatotroph-reduced sensitivity to somatostatin, somatotrophin release-inhibiting factor (SRIF), or an hypothalamic derangement of the SRIF release. In this study, this hypothesis was investigated by means of GH suppression during chronic therapy with octreotide [Somatostatin analogue (SMS)] in order to evaluate the possible correlation between GH and insulin-like growth factor 1 (IGF-1) normalization and the disappearance of these paradoxical responses in 15 acromegalic patients: 15/15 with a paradoxical GH rise after TRH and 7/15 with a paradoxical GH rise after LHRH. SMS therapy was administered subcutaneously at the dose of 150-450 micrograms/day. During the treatment, GH and IGF-1 levels normalized in 12 patients and were reduced in the remaining 3 others. The GH response to TRH disappeared in 7 patients, while the GH response to LHRH disappeared in 4 patients. chi 2 analysis failed to show any significant correlation between GH and IGF-1 normalization and the disappearance of GH response to TRH and LHRH (chi 2 = 0.00686). No linear correlation existed between GH/IGF-1 decrease and GH peak or area under the curve at any time ('r' values: TRH test, GH -0.47, IGF-1 -0.48; LHRH test, GH -0.50, IGF-1 -0.49). The absence of any significant correlation between GH/IGF-1 normalization and the disappearance of GH paradoxical responses during chronic octreotide administration suggests that other factors apart from SRIF sensitivity are involved in the genesis of these responses. 相似文献
222.
223.
B Merola E Rossi S Longobardi A Selleri V Esposito A Colao M Cataldi D Ferone G Lombardi 《Hormone research》1992,38(3-4):150-153
In our previous studies we had demonstrated that, in children affected with isolated GH deficiency (IGHD), a short-term recombinant growth hormone (rGH) therapy increases the 11-deoxycortisol (S) secretion and induces an IGF-I responsiveness to the ACTH challenge. The aim of the present study was to further investigate the mechanisms by which IGF-I is secreted after ACTH challenge in children affected with IGHD by correlating IGF-I versus cortisol (F) time courses after ACTH administration. Ten children affected with IGHD were subjected to rGH therapy (4 IU/day subcutaneously) for 10 days. The responsiveness of IGF-I, F and S to the ACTH 1-17 test were evaluated before and at the end of the therapy. No IGF-I response to the ACTH test was recorded in the patients before the rGH treatment, whereas after rGH administration ACTH induced a significant IGF-I release (p < 0.001) which started at the 1st hour, reached a peak value between the 5th and 6th hours and disappeared at the 10th hour. In conclusion, our study confirms that a short-term rGH therapy induces an IGF-I responsiveness to ACTH and helps to better define the kinetics and the mechanism of this IGF-I response to ACTH. 相似文献
224.
Niklas Feldhahn Elisabetta Ferretti Davide F Robbiani Elsa Callen Stephanie Deroubaix Licia Selleri Andre Nussenzweig Michel C Nussenzweig 《The EMBO journal》2021,40(2)
We recently became aware of panel duplications in Supplementary Figures S6 and S7, due to pasting errors of similar flow cytometry images during figure preparation. This concerned the first two panels in the top row of Suppl. Fig S6A; second and third panel in the bottom row of Suppl. Fig S7B; and third and fourth panel in the bottom row of Suppl. Fig S7C.Furthermore, we noted a typographical error in Suppl. Fig S7B (top row, sixth plot), where the indicated percentage was wrongly given as 1.4%, instead of 1.1%. These errors did not change the results or the interpretation of the data. We deeply apologize to the scientific community for any confusion these errors may have caused. The updated appendix is published with this corrigendum.The original FlowJo analysis plots related to the affected figures are published as source data with this corrigendum. Please note that initial labelling of the experiments in these files referred to the official gene name Obfc2b informally as hSSB1, and Obfc2a‐shRNAs as ‘sh1’ and sh4’.Open in a separate windowFigure S6AOriginalOpen in a separate windowFigure S6ACorrectedOpen in a separate windowFigure S7BOriginalOpen in a separate windowFigure S7BCorrectedOpen in a separate windowFigure S7COriginalOpen in a separate windowFigure S7CCorrected 相似文献
225.
Valeria Calandrelli Agata Gambacorta Ida Romano Vito Carratore Licia Lama 《World journal of microbiology & biotechnology》2008,24(10):2269-2275
A novel thermo-alkali-stable catalase–peroxidase from Oceanobacillus oncorhynchi subsp. incaldaniensis subsp. nov., strain 20AG, was purified and characterized. The protein purified from the cells resulted in 110-fold purification
with a specific activity of 35,000 U/mg. The enzyme consisted of four identical subunits of 72 kDa as determined by SDS-PAGE
and the total molecular mass measured by gel filtration was 280 kDa. The heme content was determined to be 1 heme per homodimer.
The enzyme showed a Soret peak at 406 nm in the oxidized form and was easily reduced by dithionite. The enzyme showed an appreciable
peroxidase activity in addition to high catalase activity. The behaviour of this heme-enzyme was typical of the class of prokaryotic
catalase–peroxidases, which are sensitive to cyanide and insensitive to the eukaryotic catalase inhibitor 3-amino-1,2,4-triazole.
The enzyme was active over a temperature range from 30 to 60°C and a pH range from 5 to 10, with an optimum pH about 9.0 and
an optimum temperature of 40°C. The enzyme was stable in the pH range of 5.0 to 10.0 after 1 h of treatment at 40°C. The enzyme
was stable for 24 h at 40°C with a half-life of 4 h 60°C. The enzyme had a K
m of 24 mM for hydrogen peroxide. The amino terminal amino acid sequence of the catalase–peroxidase from strain 20AG was SEKRKMTTAFGA
and it showed no homology with other catalases. 相似文献
226.
Colin Verónica Leticia Baigorí Mario Domingo Pera Licia María 《World journal of microbiology & biotechnology》2010,26(12):2291-2295
Microbial emulsifiers are compounds employed in primary mechanisms for bioremediation of petroleum and other hydrocarbon pollutants
from the environment. Although emulsifiers of biological origin are produced by microorganisms generally in response to growth
on hydrocarbons, Aspergillus niger MYA 135 produced a bioemulsifier during fermentation in a sucrose-based culture medium at an initial pH of 5.0 and at 30°C.
The production of bioemulsifiers can be strongly influenced by environmental factors. In this connection, a study of the effect
of initial pH, the incubation temperature and presence of CaCl2 or FeCl3 in the culture medium was conducted. Emulsification index was increased by 112 and 206% at an initial pH 2.0 or in medium
supplemented with FeCl3, respectively. On the other hand, emulsifying ability of Aspergillus niger supernatants was detected during the exponential phase, suggesting that bioemulsifiers accumulation and microbial growth
would be related. Interestingly, this study suggests that iron and/or phosphate ions would play a key role in maintaining
the emulsifying ability. Finally, factorial design was also employed to study the effects of the initial pH, the presence
of FeCl3 and the concentration of KH2PO4 on the emulsification index. 相似文献