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31.
LM Harris L Blank RP Desai NE Welker ET Papoutsakis 《Journal of industrial microbiology & biotechnology》2001,27(5):322-328
The effect of solR inactivation on the metabolism of Clostridium acetobutylicum was examined using fermentation characterization and metabolic flux analysis. The solR-inactivated strain (SolRH) of this study had a higher rate of glucose utilization and produced higher solvent concentrations
(by 25%, 14%, and 81%, respectively, for butanol, acetone, and ethanol) compared to the wild type. Strain SolRH(pTAAD), carrying
a plasmid-encoded copy of the bifunctional alcohol/aldehyde dehydrogenase gene (aad) used in butanol production, produced even higher concentrations of solvents (by 21%, 45%, and 62%, respectively, for butanol,
acetone, and ethanol) than strain SolRH. Clarithromycin used for strain SolRH maintenance during SolRH(pTAAD) fermentations
did not alter product formation; however, tetracycline used for pTAAD maintenance resulted in 90% lower solvent production.
Journal of Industrial Microbiology & Biotechnology (2001) 27, 322–328.
Received 12 September 2000/ Accepted in revised form 21 July 2001 相似文献
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Judith Seligman Nechama S. Kosower Ruth Shalgi 《Molecular reproduction and development》1997,47(3):295-301
Mammalian spermatozoa gain their fertilizing ability as they mature in the epididymis, a process which is accompanied by oxidation of sperm protein thiols. Since sperm maturation is dependent upon normal androgenic support to the epididymis, the present work was designed to study the effects of castration on thiol status. Spermatozoa and epididymal fluid were isolated from the epididymides of male rats 5 days after castration or after 11 daily injections of the antiandrogen, cyproterone acetate. Spermatozoa and epididymal fluid were labeled with the fluorescent thiol labeling agent monobromobimane. Intact spermatozoa were evaluated by fluorescence microscopy, protein thiols were analyzed by electrophoresis, and fertilizing ability was examined after insemination of sperm suspension into the uterine horns of immature superovulated female rats. We found that both treatments resulted in an increase in cauda sperm thiols as shown by increased fluorescence in the intact spermatozoa. Protamines and nonbasic proteins were found to have increased levels of reactive thiols. The protein profiles of epididymal fluid from castrated rats were different from those of the controls, and the fluorescence patterns corresponded to the protein profiles. Our results indicate that testosterone withdrawal leads to inhibition of sperm thiol oxidation. Mol. Reprod. Dev. 47:295–301, 1997. © 1997 Wiley-Liss, Inc. 相似文献
36.
The transferrin receptor has been isolated from human placenta using immunochromatography and affinity chromatography. The receptor is a glycoprotein and has a Mr = 90,000 on sodium dodecyl sulfate-gel electrophoresis in the presence of 2-mercaptoethanol. The isolated receptor is immunologically related to the transferrin receptor on the reticulocyte cell surface. 相似文献
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Carlos G PinheiroJr Eduardo LM Naves Pierre Pino Etienne Losson Adriano O Andrade Guy Bourhis 《Biomedical engineering online》2011,10(1):31
We have now sufficient evidence that using electrical biosignals in the field of Alternative and Augmented Communication is
feasible. Additionally, they are particularly suitable in the case of people with severe motor impairment, e.g. people with
high-level spinal cord injury or with locked-up syndrome. Developing solutions for them implies that we find ways to use sensors
that fit the user's needs and limitations, which in turn impacts the specifications of the system translating the user's intentions
into commands. After devising solutions for a given user or profile, the system should be evaluated with an appropriate method,
allowing a comparison with other solutions. This paper submits a review of the way three bioelectrical signals - electromyographic,
electrooculographic and electroencephalographic - have been utilised in alternative communication with patients suffering
severe motor restrictions. It also offers a comparative study of the various methods applied to measure the performance of
AAC systems. 相似文献