排序方式: 共有183条查询结果,搜索用时 15 毫秒
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Silvia Morbelli Cecilia Marini Gian Franco Adami Nobuyuki Kudomi Giovanni Camerini Patricia Iozzo Michela Massollo Selene Capitanio Samanta Bodrato Maria Teresa Verardi Francesco Papadia Renzo Cordera Juhani Knuuti Nicola Scopinaro Gianmario Sambuceti 《Obesity (Silver Spring, Md.)》2013,21(3):E175-E181
Objective:
The present study was planned to investigate, by means of quantitative FDG‐PET, how bariatric surgery (BS) modifies the metabolic pattern of the whole body and different tissues in slightly obese patients with type 2 diabetes mellitus (T2DM).Design and Methods:
Before, 1 and 4 months after BS, 21 consecutive slightly obese T2DM patients underwent blood sampling to estimate plasma levels of glucose, insulin, glycosylated hemoglobin. At the same time points, these patients underwent a dynamic 18F‐FDG PET study of thorax and upper abdomen in fasting state and after washout of T2DM therapy. Gjedde‐Patlak analysis was applied to estimate glucose uptake in the whole body and in different tissues (myocardium, skeletal back muscle, adipose tissue, and liver).Results:
Surgical intervention quickly lowered levels of both insulin and glucose documenting an amelioration of glucose tolerance. Similarly, skeletal muscle and myocardial glucose uptake significantly increased soon after surgery (P < 0.001 and P < 0.01 at 1 month versus baseline, respectively) and remained substantially stable thereafter. By contrast, glucose uptake slightly decreased from its baseline values in the liver (P < 0.01 at 4 months) while no response could be documented over time in the adipose tissue.Conclusions:
These findings document that BS‐induced modification of glucose homeostasis in slightly obese T2DM patients is mostly due to an increase in muscle glucose consumption. The surgically modified metabolic pattern of these patients might be of interest as a new model to investigate mechanism underlying insulin resistance. 相似文献82.
Pablo F. German Selene van der Poel Colm Carraher Andrew V. Kralicek Richard D. Newcomb 《Insect biochemistry and molecular biology》2013,43(2):138-145
Insect olfactory receptors (ORs) are a novel family of ligand-gated cation channels that can respond to volatile organic compounds at low concentrations. They are involved in the detection of odorants associated with mate recognition, food localisation and predator avoidance. These receptors form a complex that is currently thought to contain at least two subunit members: the non-canonical Orco ion channel subunit and a ligand-binding receptor subunit. The integral membrane proteins SNMP1 and 2 are also associated with olfactory function, with SNMP1 required for cis-vaccinyl acetate reception in Drosophila melanogaster. In order to investigate protein–protein interactions among these membrane proteins we measured intermolecular Förster/Fluorescence Resonance Energy Transfer (FRET) in live insect cells by acceptor photobleaching. Fusion proteins containing Cyan Fluorescent Protein or Yellow Fluorescent Protein were produced using baculovirus-mediated expression in High Five? cells. The majority of the recombinant products were of the expected size for the fusion proteins and located within intracellular membranes. We were able to show FRET efficiencies providing evidence for homomeric and heteromeric interactions of the ligand-binding OR, Or22a, and Orco (Or22a–Or22a, Or22a–Orco, Orco–Orco). There was no evidence for an interaction between SNMP1 and Orco or between SNMP2 and Orco or Or22a. However, fusion proteins of SNMP1 and Or22a did show an interaction by FRET, suggesting SNMP1 may interact with at least some insect olfactory receptor complexes. In summary, this study supports previously observed homomeric and heteromeric interactions between Orco and the ligand-binding OR, Or22a, and identifies a novel interaction between Or22a and SNMP1. 相似文献
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Dubrovsky JG Napsucialy-Mendivil S Duclercq J Cheng Y Shishkova S Ivanchenko MG Friml J Murphy AS Benková E 《The New phytologist》2011,191(4):970-983
Root system architecture depends on lateral root (LR) initiation that takes place in a relatively narrow developmental window (DW). Here, we analyzed the role of auxin gradients established along the parent root in defining this DW for LR initiation. Correlations between auxin distribution and response, and spatiotemporal control of LR initiation were analyzed in Arabidopsis thaliana and tomato (Solanum lycopersicum). In both Arabidopsis and tomato roots, a well defined zone, where auxin content and response are minimal, demarcates the position of a DW for founder cell specification and LR initiation. We show that in the zone of auxin minimum pericycle cells have highest probability to become founder cells and that auxin perception via the TIR1/AFB pathway, and polar auxin transport, are essential for the establishment of this zone. Altogether, this study reveals that the same morphogen-like molecule, auxin, can act simultaneously as a morphogenetic trigger of LR founder cell identity and as a gradient-dependent signal defining positioning of the founder cell specification. This auxin minimum zone might represent an important control mechanism ensuring the LR initiation steadiness and the acropetal LR initiation pattern. 相似文献
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Selene S. C. Nogueira Janine P. Pedroza Sérgio L. G. Nogueira‐Filho Rosana S. Tokumaru 《Ethology : formerly Zeitschrift fur Tierpsychologie》2012,118(10):1001-1009
Contact calls are used to promote cohesion among individuals that live in social groups. Capybaras are very gregarious and often vocalize. This species emits a vocalization known as a click, which has been observed during aggregating and foraging behaviors, suggesting contact or monitoring call function. We carried out a playback experiment to evaluate behavioral responses to the capybara's click call and to a bird call, used as control in ten capybaras. We compared animals' latency to respond to stimuli, the time spent in behavioral patterns, alertness, head orientation toward the sound source, and approach to the sound source. All capybaras responded to the emission of the click call playback. Most of them assumed an alert position, showed head orientation toward the sound source, and approached the sound source. They promptly reacted to the first click call emission, while few reacted to the first bird call emission, used as control. All subjects showed behavioral changes after the second emission of the click call, and some responded to the third emission. Just three individuals answered after the first control emission, while none of them responded to the second and third emission of the playback. Therefore, click call playback promotes prompt behavioral changes in capybaras, including approach to the sound source. These results indicate that this vocalization functions as a contact or monitoring call in the species. 相似文献
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Natalia Henao‐Gallego Selene Escobar‐Ramírez Zoraida Calle James Montoya‐Lerma Inge Armbrecht 《Restoration Ecology》2012,20(5):555-560
Ants are effective at moving seeds toward their nests, something that may benefit the seeds. We evaluated whether seed movements that may be useful for the rehabilitation of degraded pastures in Colombia can be enhanced by local ants. An artificial aril was prepared and then evaluated in six open cattle pasture farms. Twenty paper disks (each holding seeds with an artificial aril, honey, tuna oil, and control) were set up along linear transects at each farm, and monitored five times in 48 hours. A total of 340 out of 480 seeds were moved from the experimental units by ants. Seeds with tuna oil and an artificial aril were removed twice as frequently as the control and honey smeared seeds. Ectatomma ruidum, Solenopsis geminata, and Pheidole sp. removed the majority of seeds. Advantages of the artificial aril over tuna oil are discussed. This inexpensive technique can enhance seed movement by generalist ants in degraded pastures, likely contributing to regeneration and ecological rehabilitation. 相似文献
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Ecological impacts of feral pigs in the Hawaiian Islands 总被引:1,自引:0,他引:1
Sérgio L. G. Nogueira-Filho Selene S. C. Nogueira José M. V. Fragoso 《Biodiversity and Conservation》2009,18(14):3677-3683
The foraging habits of exotic ungulate species can directly and indirectly affect native plant and animal distribution and
abundance patterns. Most of the studies on feral pig interactions with other biota in the Hawaiian Islands have been published
as difficult to access reports to governmental and nongovernmental organizations, graduate student theses, and a few in peer
reviewed journals. In this paper we discuss the origins of pig introductions to Hawaii, their feralization process, population
expansion, and interactions with native and non-native biota. We also consider the environmental effects triggered by pigs
on local ecosystems and biotic communities. Feral pig activities can reduce the abundance of native plant species, enhance
conditions for the establishment of invasive non-indigenous plants, and perhaps indirectly negatively impact native forest
bird species. Pig foraging and traveling patterns may also lead to physical alteration of ecosystems by increasing soil erosion
that may lead to watershed degradation. However, much remains to be learned about the strength and significance of aforementioned
interactions and their long-term effects on Hawaiian biota and ecosystems due to some confounding events. Elucidating the
dynamics and long-term ecological effects generated by pigs is a crucial step towards increasing our understanding of and
more effectively managing biotic interactions. 相似文献