排序方式: 共有139条查询结果,搜索用时 15 毫秒
121.
Mariana Selena Gonzalez Carlos Daniel De Brasi Michele Bianchini Patricia Gargallo Carmen Stanganelli Ilana Zalcberg Irene Beatriz Larripa 《PloS one》2014,9(1)
Most cases of BCR-ABL1-negative myeloproliferative neoplasms (MPNs), essential thrombocythemia, polycythemia vera and primary myelofibrosis are associated with JAK2
V617F mutations. The outcomes of these cases are critically influenced by the transition from JAK2
V617F heterozygosity to homozygosity. Therefore, a technique providing an unbiased assessment of the critical allele burden, 50% JAK2
V617F, is highly desirable. In this study, we present an approach to assess the JAK2
V617F burden from genomic DNA (gDNA) and complementary DNA (cDNA) using one-plus-one template references for allele-specific quantitative-real-time-PCR (qPCR). Plasmidic gDNA and cDNA constructs encompassing one PCR template for JAK2
V617F spaced from one template for JAK2Wild Type were constructed by multiple fusion PCR amplifications. Repeated assessments of the 50% JAK2V617F burden within the dynamic range of serial dilutions of gDNA and cDNA constructs resulted in 52.53±4.2% and 51.46±4.21%, respectively. The mutation-positive cutoff was estimated to be 3.65% (mean +2 standard deviation) using 20 samples from a healthy population. This qPCR approach was compared with the qualitative ARMS-PCR technique and with two standard methods based on qPCR, and highly significant correlations were obtained in all cases. qPCR assays were performed on paired gDNA/cDNA samples from 20 MPN patients, and the JAK2
V617F expression showed a significant correlation with the allele burden. Our data demonstrate that the qPCR method using one-plus-one template references provides an improved assessment of the clinically relevant transition of JAK2
V617F from heterozygosity to homozygosity. 相似文献
122.
Rudall PJ Eldridge T Tratt J Ramsay MM Tuckett RE Smith SY Collinson ME Remizowa MV Sokoloff DD 《American journal of botany》2009,96(9):1581-1593
New data on endosperm development in the early-divergent angiosperm Trithuria (Hydatellaceae) indicate that double fertilization results in formation of cellularized micropylar and unicellular chalazal domains with contrasting ontogenetic trajectories, as in waterlilies. The micropylar domain ultimately forms the cellular endosperm in the dispersed seed. The chalazal domain forms a single-celled haustorium with a large nucleus; this haustorium ultimately degenerates to form a space in the dispersed seed, similar to the chalazal endosperm haustorium of waterlilies. The endosperm condition in Trithuria and waterlilies resembles the helobial condition that characterizes some monocots, but contrasts with Amborella and Illicium, in which most of the mature endosperm is formed from the chalazal domain. The precise location of the primary endosperm nucleus governs the relative sizes of the chalazal and micropylar domains, but not their subsequent developmental trajectories. The unusual tissue layer surrounding the bilobed cotyledonary sheath in seedlings of some species of Trithuria is a belt of persistent endosperm, comparable with that of some other early-divergent angiosperms with a well-developed perisperm, such as Saururaceae and Piperaceae. The endosperm of Trithuria is limited in size and storage capacity but relatively persistent. 相似文献
123.
Jose R. Gutierrez Alexi L. Balmuth Vardis Ntoukakis Tatiana S. Mucyn Selena Gimenez‐Ibanez Alexandra M.E. Jones John P. Rathjen 《The Plant journal : for cell and molecular biology》2010,61(3):507-518
Cytoplasmic recognition of pathogen virulence effectors by plant NB‐LRR proteins leads to strong induction of defence responses termed effector triggered immunity (ETI). In tomato, a protein complex containing the NB‐LRR protein Prf and the protein kinase Pto confers recognition of the Pseudomonas syringae effectors AvrPto and AvrPtoB. Although structurally unrelated, AvrPto and AvrPtoB interact with similar residues in the Pto catalytic cleft to activate ETI via an unknown mechanism. Here we show that the Prf complex is oligomeric, containing at least two molecules of Prf. Within the complex, Prf can associate with Pto or one of several Pto family members including Fen, Pth2, Pth3, or Pth5. The dimerization surface for Prf is the novel N‐terminal domain, which also coordinates an intramolecular interaction with the remainder of the molecule, and binds Pto kinase or a family member. Thus, association of two Prf N‐terminal domains brings the associated kinases into close promixity. Tomato lines containing Prf complexed with Pth proteins but not Pto possessed greater immunity against P. syringae than tomatoes lacking Prf. This demonstrates that incorporation of non‐Pto kinases into the Prf complex extends the number of effector proteins that can be recognized. 相似文献
124.
Roger Koukiekolo Zygmunt J. Jakubek Jenny Cheng Selena M. Sagan John Paul Pezacki 《Biophysical chemistry》2009
Eukaryotes have evolved complex cellular responses to double-stranded RNA. One response that is highly conserved across many species is the RNA silencing pathway. Tombusviruses have evolved a mechanism to evade the RNA silencing pathway that involves a small protein, p19, that acts as a suppressor of RNA silencing. This protein binds specifically to small-interfering RNAs (siRNAs) with nanomolar affinity in a sequence-independent manner and with size selectivity. 相似文献
125.
126.
Elena Rostkova Selena G. Burgess Richard Bayliss Mark Pfuhl 《Biomolecular NMR assignments》2018,12(2):221-224
The microtubule regulatory protein colonic and hepatic tumor overexpressed gene (chTOG), also known as cytoskeleleton associated protein 5 (CKAP5) plays an important role in organizing the cytoskeleton and in particular in the assembly of k-fibres in mitosis. Recently, we dissected the hitherto poorly understood C-terminus of this protein by discovering two new domains—a cryptic TOG domain (TOG6) and a smaller, helical domain at the very C-terminus. It was shown that the C-terminal domain is important for the interaction with the TACC domain in TACC3 during the assembly of k-fibres in a ternary complex that also includes clathrin. Here we now present the solution NMR assignment of the chTOG C-terminal domain which confirms our earlier prediction that it is mainly made of α-helices. However, the appearance of the 1H–15N HSQC spectrum is indicative of the presence of a considerable amount of unstructured and possibly flexible portions of protein in the domain. 相似文献
127.
Elaine M. Gould Selena J. Holmes Grant W. Tempero 《New Zealand journal of zoology.》2013,40(4):363-369
Abstract We compared the attractiveness to rats and mice of two lures, the pheromone Z‐7‐dodecen‐1 ‐y 1 acetate and peanut butter. In the first part of the study, laboratory‐bred rats and mice were placed in a Y‐maze. Each arm of the maze offered their normal food, plus either the pheromone or 5% ethanol as a control. There were no significant differences, by species or sex, in the number of visits or the amount of time spent in each arm. The male mice took significantly less time, however, to enter the control arm of the maze than the pheromone arm. In addition, female mice were less active during the first 5 min of the Y‐maze study than during the 5 min familiarisation period, suggesting a repellent effect of the pheromone lure. In the second part of the study, tracking tunnels were placed out in the field and baited with one of three lures—the pheromone, peanut butter or a 5% ethanol control. There was no significant difference in the number of visits by mice to tunnels containing the three lures, but rats made more visits to the tunnels containing peanut butter in the “Test” stage than in the “Neo” stage. The wild rats and mice were found not to be neophobic, suggesting the standard 3‐week familiarisation period is not necessary. 相似文献
128.
Jewell Dennis E. Tavener Selena K. Hollar Regina L. Panickar Kiran S. 《Metabolomics : Official journal of the Metabolomic Society》2022,18(8):1-5
Metabolomics - The global population is aging. Preserving function and independence of our aging population is paramount. A key component to maintaining independence is the preservation of... 相似文献
129.
John D. Wade Selena S. Layden Paul F. Lambert Hariclia Kakouris Geoffrey W. Tregear 《Journal of Protein Chemistry》1994,13(3):315-321
The synthesis of the hormone relaxin from the speciesGorilla gorilla (gorilla) andMacaca mulatta (rhesus monkey) has been achieved. Each of the two chains which constitute the peptide structures was assembled separately, the A-chains (24 amino acids) by the Boc-polystyrene solid-phase procedure and the B-chains (29 and 28 amino acids) by the Fmoc-polyamide (gorilla) and the Boc-polystyrene (rhesus monkey) solid-phase methods. After cleavage from the solid supports, the separate chains were purified to a high degree of homogeneity. Oxidative combination of the respective A- and B-chains in solution at highpH afforded the synthetic relaxins in low overall yield. Chemical and physiochemical characterization of the products confirmed both their purity and their conformational similarity to the human hormone. The synthetic gorilla and rhesus monkey relaxins were both found to possess potent chronotropic and inotropic activity in the isolated rat cardiac atrium assay. 相似文献
130.
Xiaoyun Wu Gavin R. Schnitzler Galen F. Gao Brett Diamond Andrew R. Baker Bethany Kaplan Kaylyn Williamson Lindsay Westlake Selena Lorrey Timothy A. Lewis Colin W. Garvie Martin Lange Sikander Hayat Henrik Seidel John Doench Andrew D. Cherniack Charlotte Kopitz Matthew Meyerson Heidi Greulich 《The Journal of biological chemistry》2020,295(48):16464