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排序方式: 共有221条查询结果,搜索用时 15 毫秒
91.
Seitaro Mutoh Ikuo Funakoshi Nobuo Ui Ikuo Yamashina 《Archives of biochemistry and biophysics》1980,202(1):137-143
A proteoglycan isolated from plasma membranes of an ascites hepatoma, AH 66, was characterized structurally. The glycosaminoglycan was obtained by alkali treatment and was identified as heparan sulfate. It was essentially the only type of carbohydrate chain attached to the core protein. The identification was based on chemical analysis, electrophoresis, and digestibility with heparitinase from Flavobacterium heparinum. Analysis of neutral sugars of the proteoglycan by mass fragmentography indicated the presence of xylose and galactose which should be involved in the linkage region between a heparan sulfate chain and the core protein. The weight-average molecular weights of the proteoglycan and its heparan sulfate chain were determined to be 71,000 and 21,000, respectively, by meniscus depletion equilibrium centrifugation. The latter value was in good agreement with those obtained by chemical analysis and by gel filtration. From these values for molecular weight and the protein content of the proteoglycan (10.6%), the molecular weight of the core protein was estimated to be 7500. On the basis of these molecular parameters, it was proposed that three heparan sulfate chains on average are linked to the core protein. 相似文献
92.
We obtained a monoclonal antibody (MA-1) specific for macronuclei of the ciliate Paramecium caudatum and P. dubosqui. Immunoblotting showed that the antigen was a polypeptide of 50 kilodalton (kDa). During the process of nuclear differentiation in P. caudatum, the MA-1 antigens appeared in the macronuclear anlagen immediately after four out of eight post zygotic nuclei differentiated morphologically into the macronuclear anlagen. Afterwards, the antigens could be detected in the macronucleus through the cell cycle, and disappeared when the macronucleus began to degenerate in exconjugant cells. These results suggest that the antigens may play a role in the differentiation and function of the macronucleus. 相似文献
93.
T Yamada A Fujishima K Kawahara K Kato O Nishimura 《Archives of biochemistry and biophysics》1987,257(1):194-199
Recombinant human interleukin-2 (rIL-2) produced in Escherichia coli possesses a free thiol group at Cys-125 and a disulfide linkage between Cys-58 and Cys-105, as in the case for natural human interleukin-2. Treatment of rIL-2 with 200 mM dithiothreitol resulted in the cleavage of the Cys-58-Cys-105 disulfide bond. The reduced form of rIL-2 thus obtained retained only 10% of the in vitro biological activity of the native form, as measured by the ability to stimulate the growth of an IL-2-dependent mouse natural killer cell line, NKC3. Far-uv circular dichroism studies indicated that the cleavage of the disulfide bond results in a decrease of alpha-helix content. Near-uv circular dichroism studies suggested that the native molecule is folded into a rigid tertiary structure, while the reduced form showed a spectrum similar to that of rIL-2 denatured in the presence of 6 M guanidine.HCl. The once-reduced molecule was readily reoxidized in the presence of 10 microM Cu2+ to form the native molecule with full biological activity. These results strongly demonstrate that the Cys-58-Cys-105 disulfide linkage in the IL-2 molecule is essential for constructing a rigid and biologically active form of IL-2. 相似文献
94.
Interaction of calmodulin with troponin I and the troponin-tropomyosin-actin complex. Effect of Ca2+ and Sr2+ ions.
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In an effort to elucidate the mechanism of calmodulin regulation of muscle contraction, we investigated the interaction between calmodulin and troponin components in the presence of Ca2+ or Sr2+ by the use of ultracentrifugation methods and polyacrylamide-gel electrophoresis. Skeletal-muscle troponin C bound to troponin I and dissociated it from the tropomyosin-actin complex in the presence of Ca2+ or Sr2+. When troponin T was absent, calmodulin bound to troponin I and dissociated it from the tropomyosin-actin complex in the presence of Ca2+ or Sr2+. When troponin T was present, calmodulin hardly bound to troponin I even in the presence of bivalent cations. Trifluoperazine, a calmodulin antagonist, inhibited the bivalent-cation-dependent interaction between calmodulin and troponin I. Calmodulin migrated more slowly in the presence of Sr2+ than it did in the presence of EGTA but faster than it did in the presence of Ca2+ on polyacrylamide-gel electrophoresis under non-denaturing conditions. It is concluded that troponin T is not required in the calmodulin regulation of muscle contraction because troponin T inhibits the bivalent-cation-dependent interaction between calmodulin and troponin I and because calmodulin binds to troponin I and dissociates it from the tropomyosin-actin complex in a bivalent-cation-dependent manner. Sr2+-induced exposure of the hydrophobic region enables calmodulin to bind to troponin I, as is the case with Ca2+. 相似文献
95.
Summary. Each symbiotic Chlorella sp. of the ciliate Paramecium bursaria is enclosed in a perialgal vacuole derived from the host digestive vacuole, and thereby the alga is protected from digestion
by lysosomal fusion. Algae-free cells can be reinfected with algae isolated from algae-bearing cells by ingestion into digestive
vacuoles. To examine the timing of acidification and lysosomal fusion of the digestive vacuoles and of algal escape from the
digestive vacuole, algae-free cells were mixed with isolated algae or yeast cells stained with pH indicator dyes at 25 ± 1
°C for 1.5 min, washed, chased, and fixed at various time points. Acidification of the vacuoles and digestion of Chlorella sp. began at 0.5 and 2 min after mixing, respectively. All single green Chlorella sp. that had been present in the host cytoplasm before 0.5 h after mixing were digested by 0.5 h. At 1 h after mixing, however,
single green algae reappeared in the host cytoplasm, arising from those digestive vacuoles containing both nondigested and
partially digested algae, and the percentage of such cells increased to about 40% at 3 h. At 48 h, the single green algae
began to multiply by cell division, indicating that these algae had succeeded in establishing endosymbiosis. In contrast to
previously published studies, our data show that an alga can successfully escape from the host’s digestive vacuole after acidosomal
and lysosomal fusion with the vacuole has occurred, in order to produce endosymbiosis.
Correspondence and reprints: Biological Institute, Faculty of Science, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8512,
Japan. 相似文献
96.
Discovery of quinazolinone and quinoxaline derivatives as potent and selective poly(ADP-ribose) polymerase-1/2 inhibitors 总被引:2,自引:0,他引:2
Iwashita A Hattori K Yamamoto H Ishida J Kido Y Kamijo K Murano K Miyake H Kinoshita T Warizaya M Ohkubo M Matsuoka N Mutoh S 《FEBS letters》2005,579(6):1389-1393
Two classes of quinazolinone derivatives and quinoxaline derivatives were identified as potent and selective poly(ADP-ribose) polymerase-1 and 2 (PARP-1) and (PARP-2) inhibitors, respectively. In PARP enzyme assays using recombinant PARP-1 and PARP-2, quinazolinone derivatives displayed relatively high selectivity for PARP-1 and quinoxaline derivatives showed superior selectivity for PARP-2. SBDD analysis via a combination of X-ray structural study and homology modeling suggested distinct interactions of inhibitors with PARP-1 and PARP-2. These findings provide a new structural framework for the design of selective inhibitors for PARP-1 and PARP-2. 相似文献
97.
T. Eki M. Abe K. Furuya I. Ahmad N. Fujishima H. Kishida A. Shiratori T. Onozaki K. Yokoyama D. Le Paslier D. Cohen F. Hanaoka Y. Murakami 《Mammalian genome》1996,7(4):303-311
The human Chromosome (Chr) 21q22.1 region contains several genes for cytokines and neurotransmitters and the gene for superoxide
dismutase (mutant forms of which can cause familial amyotrophic lateral sclerosis). A region of approximately 5.8 Mb encompassing
D21S82 and the glycinamide ribonucleotide transformylase (GART) loci was covered by overlapping YAC clones, which were contiguously
ordered by clone walking with sequence-tagged site (STSs). A total of 76 markers, including 29 YAC end-specific STSs, were
unambiguously ordered in this 5.8-Mb region, and the average interval between markers was 76 kb. Restriction maps of the YAC
clones with rare-cutting enzymes were simultaneously prepared, and the restriction sites were aligned to obtain a consensus
restriction map of the proximal region of the 21q22.1 band. The restriction map made from 44 overlapping YACs contains 54
physically assigned STSs. By integrating the consensus map of the adjacent 1.8-Mb region, we obtained a fine physical map
spanning 6.5 Mb of human Chr 21q22.1. This map contains 24 precisely positioned end-specific STSs and 12 NotI-linking markers. More than 39 potential CpG islands were identified in this region and were found to be unevenly distributed.
This physical map and the YACs should be useful as a reference map and as a resource for further structural analysis of the
Giemsa-negative band (R-band) of Chr 21q22.1.
Received: 1 September 1995 / Accepted: 21 November 1995 相似文献
98.
Tomihiro Shimizu Mitsuo Kosaka Kazutaka Fujishima 《European journal of applied physiology and occupational physiology》1998,78(6):473-478
Eight healthy and physically well-trained male students exercised on a treadmill for 60 min while being immersed in water
to the middle of the chest in a laboratory flowmill. The water velocity was adjusted so that the intensity of exercise correspond
to 50% maximal oxygen uptake of each subject, and experiments were performed once at each of three water temperatures: 25,
30, 35°C, following a 30-min control period in air at 25°C, and on a treadmill in air at an ambient temperature of 25°C. Thermal
states during rest and exercise were determined by measuring rectal and skin temperatures at various points, and mean skin
temperatures were calculated. The intensity of exercise was monitored by measuring oxygen consumption, and heart rate was
monitored as an indicator for cardiovascular function. At each water temperature, identical oxygen consumption levels were
attained during exercise, indicating that no extra heat was produced by shivering at the lowest water temperature. The slight
rise in rectal temperature during exercise was not influenced by the water temperature. The temperatures of skin exposed to
air rose slightly during exercise at 25°C and 30°C water temperature and markedly at 35°C. The loss of body mass increased
with water temperature indicating that both skin blood flow and sweating during exercise increased with the rise in water
temperature. The rise in body temperature provided the thermoregulatory drive for the loss of the heat generated during exercise.
Heart rate increased most during exercise in water at 35°C, most likely due to enhanced requirements for skin blood flow.
Although such requirements were certainly smallest at 25°C water temperature, heart rate at this temperature was slightly
higher than at 30°C suggesting reflex activation of sympathetic control by cold signals from the skin. There was a significantly
greater increase in mean skin and rectal temperatures in subjects exercising on the treadmill in air, compared to those exercising
in water at 25°C.
Accepted: 22 May 1998 相似文献
99.
T Karashima K Kato H Nawata S Ikuyama H Ibayashi K Okamura A Shiroozu T Nakashima M Fujishima 《Endocrinologia japonica》1986,33(2):163-167
The value of predictive tests in bromocriptine therapy and the effects of long-term bromocriptine therapy were investigated in acromegalic patients. In 72 acromegalic patients, there was a tendency for patients with a plasma GH response to TRH or with an elevated basal plasma PRL level, but without a plasma GH response to LHRH, to have a plasma GH response to bromocriptine, though statistical analysis did not reveal a significant difference. Acute and chronic effects of bromocriptine were significantly interrelated, while the chronic effect of bromocriptine and abnormal plasma GH response to TRH or elevated plasma PRL levels were not, in 18 acromegalic patients. These results suggest that the acute bromocriptine test is a better predictor than the TRH test and plasma PRL levels for evaluating the effects of chronic bromocriptine therapy. To maintain the low plasma GH levels, increasing doses of bromocriptine were needed in most patients, and failure to control the elevated GH level despite increasing doses was observed in 2 of 18 patients. 相似文献
100.
Hiroshi Yao Takashi Matsumoto Makoto Hirano Toshihide Kuroki Tetsuyuki Tsutsumi Hideyuki Uchimura Kaoru Nakamura Tatsuo Nakahara Masatoshi Fujishima 《Neurochemical research》1989,14(1):75-79
This study attempted to investigate the possible involvement of the brain stem noradrenergic system in the development of hypertension in spontaneously hypertensive rats. Steady-state norepinephrine, dopamine, serotonin and 5-hydroxyindoleacetic acid concentrations and norepinephrine turnover were determined in the individual brain stem nuclei using high performance liquid chromatography with electrochemical detection. Decreased norepinephrine contents in the nucleus tractus solitarii in spontaneously hypertensive rats compared with Wistar-Kyoto rats at the age of 4, 8, and 16 weeks were demonstrated. In later stages (8 and 16 weeks), increased norepinephrine levels were observed in the nucleus reticularis gigantocellularis, the A1 and A5 areas. Norepinephrine turnover was not different between spontaneously hypertensive rats and Wistar-Kyoto rats in the nucleus tractus solitarii at the age of 4 and 16 weeks and increased in the nucleus reticularis gigantocellularis of spontaneously hypertensive rats at 16 weeks. Our results indicate that altered norepinephrine metabolism in the specific brain stem nuclei, especially the consistently decreased norepinephrine in the nucleus tractus solitarii of spontaneously hypertensive rats, contribute to the development of genetic hypertension. 相似文献