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111.
112.
Tsutomu Araki Hiroyuki Kato Yasuo Kanai Kyuya Kogure 《Neurochemistry international》1993,23(6):541-548
Age-related alterations in major neurotransmitter receptors and voltage dependent calcium channels were analyzed by receptor autoradiography in the gerbil brain. [3H]Quinuclidinyl benzilate (QNB). [3H]cyclohexyladenosine (CHA), [3H]muscimol, [3H]MK-801, [3H]SCH 23390, [3H]naloxone, and [3H]PN200-110 were used to label muscarinic acetylcholine receptors, adenosine A1 receptors, γ-aminobutyric acidA (GABAA) receptors,
(NMDA) receptors, dopamine D1 receptors, opioid receptors, and voltage dependent calcium channels, respectively. In middle-aged gerbils (16 months old), the hippocampus exhibited a significant elevation in [3H]QNB, [3H]MK-801, [3H]SCH 23390, [3H]naloxone, and [3H]PN200-110 binding, whereas [3H]CHA and [3H]muscimol binding showed a significant reduction in this area, compared with that of young animals (1 month). On the other hand, the cerebellum showed a significant alteration in [3H]QNB, [3H]CHA, and [3H]naloxone binding and the striatum also exhibited a significant alteration in [3H]SCH 23390 and [3H]CHA binding in middle-aged gerbils. The neocortex showed a significant elevation only in [3H]CHA binding in middle-aged animals. The nucleus accumbens and thalamus also showed a significant alteration only in [3H]muscimol binding. However, the hypothalamus and substantia nigra exhibited no significant alteration in these bindings in middle-aged gerbils. These results demonstrate the age-related alterations of various neurotransmitter receptors and voltage dependent calcium channels in most brain regions. Furthermore, they suggest that the hippocampus is most susceptible to aging processes and is altered at an early stage of senescence. 相似文献
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114.
Hydrobiologia - During larval development of the intertidal barnacle Fistulobalanus albicostatus, larvae in the naupliar stages I and II (NI&II) possess a single naupliar eye, and later... 相似文献
115.
Although the ecology of many exotic invaders has been intensively examined in the novel range, few studies have comparatively
explored how population dynamics differ in native and novel parts of an invading plants’ range. The population dynamics of
mile-a-minute weed, Polygonum perfoliatum L., was explored in both the native (Japan) and novel (northeastern USA) portions of its range and evaluated using periodic
matrix models. Projected per capita population growth rate (λ) varied within and between native and novel range populations.
Surprisingly, five of the six populations in the novel range were projected to fail to replace themselves (λ<1) while only
two of the four native range populations were projected to decline, although these projections had wider confidence intervals
than in the novel habitat. While changes in germination, survivorship, fecundity and seed banking would have equivalent effects
on population growth in the invasive habitat, small increases in plant survivorship would greatly increase λ in native populations.
The differences between native and novel population growth rates were driven by lower adult survival in the native range caused
by annual flooding and higher fecundity. Simulation analyses indicated that a 50% reduction in plant survival would be required
to control growing populations in the novel range. Further comparative studies of other invading species in both their native
and novel ranges are needed to examine whether the high per capita population growth and strong regulatory effects of adult
survival in the native habitat are generally predictive of invasive behavior in novel habitats.
Sachiko Araki: (Deceased) 相似文献
116.
Gazza squamiventralis sp. nov. is described as the fifth species of the genus, based on the holotype and eight paratypes, 42–96 mm in standard
length, collected along the east coast of Africa, from Kenya to Mozambique. The species is similar to other congeners in general
appearance, differing clearly from them in having the ventrolateral surface of the body scaled anterior to a line from the
pectoral fin base to the pelvic fin origin (vs naked) and a smooth supraorbital ridge (vs finely serrated). Additionally,
the species differs from G. dentex, G. rhombea, and G. achlamys in having the dorsolateral surface of the body scaled anterior to a vertical through the tip of the posterior branch of the
supratemporal canal (vs corresponding region naked), and from G. minuta in having the first dorsal fin pterygiophore narrowly expanded anteriorly, with a concave margin (vs broadly expanded, with
a convex or linear margin), and a short antrorse extension of the first anal fin pterygiophore (vs long). A key to the five
species of the genus Gazza is provided.
Received: May 30, 2000 / Revised: September 16, 2000 / Accepted: January 16, 2001 相似文献
117.
Hori Y Bichenkova EV Wilton AN El-Attug MN Sadat-Ebrahimi S Tanaka T Kikuchi Y Araki M Sugiura Y Douglas KT 《Biochemistry》2001,40(3):603-608
2,2'-p-Phenylene bis[6-(4-methyl-1-piperazinyl)]benzimidazole, 2,2'-bis(3,5-dihydroxyphenyl)-6,6'-bis benzimidazole, and 2,2'-bis(4-hydroxyphenyl)-6,6'-bis benzimidazole are shown by UV-visible and fluorescence spectrophotometry to be strong ligands for tRNA, giving simple, hyperbolic binding isotherms with apparent dissociation constants in the micromolar range. Hydroxyl radical footprinting indicates that they may bind in the D and T loops. On the basis of this tRNA recognition as a rationale, they were tested as inhibitors of the processing of precursor tRNAs by the RNA subunit of Escherichia coli RNase P (M1 RNA). Preliminary studies show that inhibition of the processing of Drosophila tRNA precursor molecules by phosphodiester bond cleavage, releasing the extraneous 5'-portion of RNA and the mature tRNA molecule, was dependent on both the structure of the inhibitor and the structure of the particular tRNA precursor substrate for tRNA(Ala), tRNA(Val), and tRNA(His). In more detailed followup using the tRNA(His) precursor as the substrate, experiments to determine the concentration dependence of the reaction showed that inhibition took time to reach its maximum extent. I(50) values (concentrations for 50% inhibition) were between 5.3 and 20.8 microM, making these compounds among the strongest known inhibitors of this ribozyme, and the first inhibitors of it not based on natural products. These compounds effect their inhibition by binding to the substrate of the enzyme reaction, making them examples of an unusual class of enzyme inhibitors. They provide novel, small-molecule, inhibitor frameworks for this endoribonuclease ribozyme. 相似文献
118.
Araki H Li Y Yamamoto Y Haneda M Nishi K Kikkawa R Ohkubo I 《Journal of biochemistry》2001,129(2):279-288
We purified dipeptidyl peptidase II (DPP II) to homogeneity from rat kidney and determined its physicochemical properties, including its molecular weight, substrate specificity, and partial amino acid sequence. Furthermore, we screened a rat kidney cDNA library, isolated the DPP II cDNA and determined its structure. The cDNA was composed of 1,720 base pairs of nucleotides, and 500 amino acid residues were predicted from the coding region of cDNA. Human quiescent cell proline dipeptidase (QPP) cloned from T-cells is a 58-kDa glycoprotein existing as a homodimer formed with a leucine zipper motif. The levels of amino acid homology were 92.8% (rat DPP II vs. mouse QPP) and 78.9% (rat DPP II vs. human QPP), while those of nucleotide homology were 93.5% (rat DPP II vs. mouse QPP) and 79.4% (rat DPP II vs. human QPP). The predicted amino acid sequences of rat DPP II and human and mouse QPP possess eight cysteine residues and a leucine zipper motif at the same positions. The purified DPP II showed similar substrate specificity and optimal pH to those of QPP. Consequently, it was thought that DPP II is identical to QPP. Northern blot analysis with rat DPP II cDNA revealed prominent expression of DPP II mRNA in the kidney, and the order for expression was kidney > testis > or = heart > brain > or = lung > spleen > skeletal muscle > or = liver. In parallel with Northern blot analysis, the DPP II antigen was detected by immunohistochemical staining in the cytosol of epithelial cells in the kidney, testis, uterus, and cerebrum. 相似文献
119.
A soluble enzyme activity that attaches free diaminopimelic acid to bdelloplast peptidoglycan 总被引:1,自引:0,他引:1
An enzyme activity, responsible for the attachment of diaminopimelic acid (DAP) to bdelloplast wall peptidoglycan, was studied in an in vitro, cell-free system. Most of the activity was found in the high-speed (20000g) supernatant fraction of homogenates of bdelloplasts prepared from a culture of the intracellular bacterium Bdellovibrio bacteriovorus 109J, growing synchronously within cells of Escherichia coli. Peptidoglycan preparations obtained either from E. coli ML35 or from the walls of bdelloplasts synchronously cultured for 40 or 90 min served as the acceptors in this reaction, whereas cell wall or peptidoglycan preparations obtained from Gram-positive bacteria could not function as acceptors of DAP. The attachment activity had an apparent Km value for DAP of 10 microM; for bdelloplast peptidoglycan, it was approximately 0.43 mg/mL, which is 13 microM with respect to peptidoglycan disaccharide peptide units. DAP attachment was partially inhibited by the structural analogues lanthionine, L-ornithine, beta-aminobutyric acid, and D-serine, as well as the cell wall synthesis inhibitors penicillin G, ampicillin, and cephalexin. This enzyme activity is present only during the intracellular stage of the bdellovibrio's developmental growth cycle and may serve a stage-specific function of biochemically modifying the cell in which it grows. 相似文献
120.