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991.
Ikuko Kondo Takeki Hirano Hideo Hamaguchi Yoshiaki Ohta Sachiko Haibara Hiroshi Nakai Hitoshi Takita 《Human genetics》1979,46(2):141-147
Summary An infant with karyotype 46,XY,der(8),t(3;8)(q21;p23) is presented. The presence of trisomy 3q21qter syndrome is suspected on the basis of comparison of the clinical and laboratory findings of this patient with those of cases that have been reported as partial 3q trisomy. The common phenotypic features of this syndrome include growth failure and mental or developmental retardation, hypotonia, persistent lanugo, distorted head, congenital glaucoma, short and upturned nose, prominent maxilla, micrognathia, short, webbed neck, short limbs, retroflexed third and fourth toes, cutaneous syndactyly of the second, third and fourth toes, and elevated galactose-1-phosphate uridyl transferase activity in the red blood cells. 相似文献
992.
H. Tokura M. Shimomoto T. Tsurutani T. Ohta 《International journal of biometeorology》1978,22(4):271-278
Body weight loss was measured continuously for 24 hours in lightly clothed subjects at a room temperature of 26°C and relative humidity of 60% using the bed scale (model 33B, James Addison Potter). One male, aged 38 years and four females, aged 22 years served as subjects. Skin temperatures of the chest, thigh, leg and arm were measured every ten min. and mean skin temperature was calculated. Water loss through the skin was estimated by deducting body weight loss due to respiratory gas exchange and respiratory water loss from total weight loss. Respiratory water loss was estimated by using the formula
(44-Pa) where
e is the rate of evaporative water loss in the expired air (g/min),
the oxygen uptake (L/min STPD) and Pa is the ambient water vapor pressure. Insensible water loss through skin was found to be significantly lower between 2:00–5:00 h than in the daytime. These findings contradict the results that the value of insensible water loss was higher at night than during the day. 相似文献
993.
Under hydrolytic conditions using 6 M HCl, tryptophan reacted separately with dithiodiglycolic acid and cystine to give beta-3-oxindolylalanine (beta-[3-(2-indolinone)]alanine) as the main product. A compound, which eluted in the amino acid analyzer at the same position as beta-3-oxindolylalanine, was found in the acid hydrolyzate of lysozyme. The identicalness of these two compounds was established by comparison of their ultraviolet absorption spectra, elution positions on ion exchange chromatograms, etc. The "acid degradation product of tryptophan", which is known to be produced upon acid hydrolysis of tryptophan-containing proteins, must also be the same compound. 相似文献
994.
Gene clusters for ribosomal proteins in the mitochondrial genome of a liverwort, Marchantia polymorpha. 总被引:2,自引:2,他引:0
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M Takemura K Oda K Yamato E Ohta Y Nakamura N Nozato K Akashi K Ohyama 《Nucleic acids research》1992,20(12):3199-3205
We detected 16 genes for ribosomal proteins in the complete sequence of the mitochondrial DNA from a liverwort, Marchantia polymorpha. The genes formed two major clusters, rps12-rps7 and rps10-rpl2-rps19-rps3-rpl16-rpl5- rps14-rps8- rpl6-rps13-rps11-rps1, very similar in organization to Escherichia coli ribosomal protein operons (str and S10-spc-alpha operons, respectively). In contrast, rps2 and rps4 genes were located separately in the liverwort mitochondrial genome (the latter was part of the alpha operon in E. coli). Furthermore, several ribosomal proteins encoded by the liverwort mitochondrial genome differed substantially in size from their counterparts in E. coli and liverwort chloroplast. 相似文献
995.
The secretory enzyme extracellular-superoxide dismutase (EC-SOD) has affinity for heparin and some other sulfated glycosaminoglycans and is in vivo bound to heparan sulfate proteoglycan. Nonenzymic glycation of EC-SOD, both in vivo and in vitro, is associated with a reduction in heparin affinity, whereas the enzymic activity is not affected. The glycation sites in EC-SOD are further studied in the present article. It is shown that modification of a few of the five lysyl residues of the subunits of the enzyme with trinitrobenzene sulfonic acid nearly abolishes the in vitro glycation susceptibility. From a chymotryptic digest of in vitro glycated EC-SOD, two peptides with affinity for boronate could be isolated. Amino acid sequence analysis showed that both encompassed the carboxyterminal end. epsilon-Glucitol lysine was identified in both peptides at positions 211 and 212. The primary glycation sites in EC-SOD are thus lysine-211 and lysine-212 in the putative heparin-binding domain in the carboxyterminal end. 相似文献
996.
Manabu Ogiso Masako Ohta Yasushi Harada Shusuke Hirano 《Journal of neurochemistry》1992,59(2):636-643
Dissociated neuronal cells from rat embryonic hemispheres were cultivated on astroglial layers. The increase in ganglioside content of the cocultures was more rapid than that of neuronal cultures seeded on polylysine surfaces for the first 24 h, and the extent of the increase was greater 7 days after inoculation, probably because of interaction between the preformed astroglial layers and the neuronal cells in vitro. The promoted expression of the a-pathway gangliosides, GM1 and GD1a, was recognized by TLC and the increase in GM1 was immunologically ascertained. The incorporation of 3H-labeled N-acetyl-D-mannosamine into GD3 and b-series gangliosides was elevated for the first 24 h. However, cocultures in which there was no contact between neuronal cells and the astroglial sheet showed no appreciable increase in incorporation. Thus, cell surface changes were induced at the membrane glycolipid level in the neuronal cells by contact with astroglial layers. The synthesis and expression of neuronal gangliosides are discussed in relation to the onset of neuron--glia interaction. 相似文献
997.
Y F Sasaki H Yamada K Shimoi N Kinae I Tomita H Matsumura T Ohta Y Shirasu 《Mutation research》1992,269(1):79-95
The effects of post-treatment with heterocyclic amines and beta-carbolines on the induction of chromosome aberrations were studied in Chinese hamster CHO K-1 cells and SV40-transformed excision repair-deficient human XP2OSSV cells. The number of chromosome aberrations induced by UV and MMC were increased by post-treatment with Trp-P-1 and Trp-P-2, in both the presence and the absence of S9 mix. A alpha C, MeA alpha C, Glu-P-1, Glu-P-2, IQ, MeIQ, harman and harmine increased chromosome aberrations only in the presence of S9 mix. Glu-P-2, IQ, MeIQ, harman, and harmine did not induce chromosome aberrations by themselves at the concentrations used in this study. Trp-P-1, Trp-P-2, A alpha C, MeA alpha C and Glu-P-1 were weak clastogens by themselves, but at much higher concentrations than those at which they increased the induction of chromosome aberrations in cells pretreated with UV or MMC. Therefore, the increases in chromosome aberrations were not considered to be additive. 相似文献
998.
Nakamura Yoshiyuki; Kasamo Kunihiro; Sakata Makato; Ohta Eiji 《Plant & cell physiology》1992,33(2):139-149
Extrusion of protons as a response to high-NaCl stress in intactmung bean roots was investigated at different external concentrationsof Ca2+ ions ([Ca2+]ex). The extrusion of protons was graduallyenhanced in the roots exposed to 100 mM NaCl, and high [Ca2+]exdiminished this enhancement of the extrusion. Vesicles of plasmalemmaand tonoplast were prepared from the roots and the H+-translocatingATPase (H+-ATPase) activities associated with the two typesof membrane and the H+-pyrophosphatase (H+-PPase) activity ofthe tonoplast were assayed. The plasmalemma ATPase was stimulatedin parallel with dramatic increases in the intracellular concentrationof Na+([Na+]in). High [Ca2+]ex prevented the increase in [Na+]inand diminished the stimulation of ATPase activity. The tonoplastATPase showed a rapid response to salt stress and was similarlystimulated even at high [Ca2+]M. The activities of both ATPaseswere, however, insensitive to concentrations of Na+ ions upto 100 HIM. By contrast, H+-PPase activity of the tonoplastwas severely inhibited with increasing [Na+]in under salt stressand recovered with high [Ca2+]ex. These findings suggest thathigh-NaCl stress increases the intracellular concentration ofNa+ ions in mung bean roots, which inhibits the tonoplast H+-PPase,and the activity of the plasmalemma H+-ATPase is thereby stimulatedand regulates the cytoplasmic pH. (Received March 26, 1991; Accepted December 13, 1991) 相似文献
999.
Purification and characterization of a thermostable carboxypeptidase (carboxypeptidase Taq) from Thermus aquaticus YT-1. 总被引:3,自引:0,他引:3
S H Lee E Minagawa H Taguchi H Matsuzawa T Ohta S Kaminogawa K Yamauchi 《Bioscience, biotechnology, and biochemistry》1992,56(11):1839-1844
A thermostable carboxypeptidase, which we named carboxypeptidase Taq, was purified from Thermus aquaticus YT-1 and characterized. The molecular weight of the enzyme was estimated to be about 56,000 and 58,000 on SDS-polyacrylamide gel electrophoresis and gel filtration, respectively, indicating that the enzyme has a monomeric structure. The optimum pH of the enzyme was 8.0, and the optimum temperature for the reaction was 80 degrees C. The enzyme activity was dependent on cobalt ion and was inhibited by metal-chelating reagents, indicating that the enzyme is a metalloenzyme. The enzyme had high thermostability independent of cobalt ion; about 90% of its activity remained even after treatment at 80 degrees C for 5 h. The enzyme showed broad substrate specificity, although proline at the C-terminus of peptides was not cleaved. The enzyme released amino acids sequentially from the C-terminus. 相似文献
1000.
Y Ogura M Takemura K Oda K Yamato E Ohta H Fukuzawa K Ohyama 《Bioscience, biotechnology, and biochemistry》1992,56(5):788-793
A gene, frxC, which is unique to the chloroplast genome of the liverwort Marchantia polymorpha, has sequence similarity to nifH, the product of which is an iron protein of a nitrogenase. Although frxC is expressed to produce a protein in liverwort chloroplasts, its function is not known. Using a probe of liverwort chloroplast DNA, a 10.1-kb region containing a gene cluster consisting of open reading frames (ORF278-frxC-ORF469-ORF248) was isolated from the cyanobacterium Synechocystis PCC6803. In this region, frxC and ORF469 showed sequence similarities to liverwort chloroplast frxC (83%) and immediately downstream ORF465 (74%), respectively. Synechocystis frxC showed 31% amino acid sequence identity with nifH1 from Clostridium pasteurianum. Additionally, Synechocystis ORF469 showed a sequence similarity (19% identity) to C. pasteurianum nifK product, which is the beta subunit of a molybdenum-iron protein of a nitrogenase complex. Conservation of the gene arrangement between liverwort and Synechocystis suggests that the liverwort chloroplast frxC-ORF465 cluster may have evolved from an ancestor common to Synechocystis, and that these two genes may have been transferred to the nuclear genome in tobacco and rice during evolution. 相似文献