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991.
Shota Yamauchi Kenji Takeuchi Kazuyasu Chihara Xuedong Sun Chisato Honjoh Hatsumi Yoshiki Hak Hotta Kiyonao Sada 《The Journal of biological chemistry》2015,290(36):21857-21864
Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) is thought to regulate the replication of viral RNA and the assembly of virus particles in a serine/threonine phosphorylation-dependent manner. However, the host kinases that phosphorylate NS5A have not been fully identified. Here, we show that HCV particle assembly involves the phosphorylation of NS5A by the c-Abl tyrosine kinase. Pharmacological inhibition or knockdown of c-Abl reduces the production of infectious HCV (J6/JFH1) particles in Huh-7.5 cells without markedly affecting viral RNA translation and replication. NS5A is tyrosine-phosphorylated in HCV-infected cells, and this phosphorylation is also reduced by the knockdown of c-Abl. Mutational analysis reveals that NS5A tyrosine phosphorylation is dependent, at least in part, on Tyr330 (Tyr2306 in polyprotein numbering). Mutation of this residue to phenylalanine reduces the production of infectious HCV particles but does not affect the replication of the JFH1 subgenomic replicon. These findings suggest that c-Abl promotes HCV particle assembly by phosphorylating NS5A at Tyr330. 相似文献
992.
Spermatogenesis is a key developmental process allowing for a formation of a mature male gamete. During its final phase, spermiogenesis, haploid round spermatids undergo cellular differentiation into spermatozoa, which involves extensive restructuring of cell morphology, DNA, and epigenome. Using mouse models with abrogated Y chromosome gene complements and Y-derived transgene we identified Y chromosome encoded Zfy2 as the gene responsible for sperm formation and function. In the presence of a Zfy2 transgene, mice lacking the Y chromosome and transgenic for two other Y-derived genes, Sry driving sex determination and Eif2s3y initiating spermatogenesis, are capable of producing sperm which when injected into the oocytes yield live offspring. Therefore, only three Y chromosome genes, Sry, Eif2s3y and Zfy2, constitute the minimum Y chromosome complement compatible with successful intracytoplasmic sperm injection in the mouse. 相似文献
993.
Songlin Wang Sudhakar Parthasarathy Yusuke Nishiyama Yuki Endo Takahiro Nemoto Kazuo Yamauchi Tetsuo Asakura Mitsuhiro Takeda Tsutomu Terauchi Masatsune Kainosho Yoshitaka Ishii 《PloS one》2015,10(4)
We present a general approach in 1H-detected 13C solid-state NMR (SSNMR) for side-chain signal assignments of 10-50 nmol quantities of proteins using a combination of a high magnetic field, ultra-fast magic-angle spinning (MAS) at ~80 kHz, and stereo-array-isotope-labeled (SAIL) proteins [Kainosho M. et al., Nature 440, 52–57, 2006]. First, we demonstrate that 1H indirect detection improves the sensitivity and resolution of 13C SSNMR of SAIL proteins for side-chain assignments in the ultra-fast MAS condition. 1H-detected SSNMR was performed for micro-crystalline ubiquitin (~55 nmol or ~0.5mg) that was SAIL-labeled at seven isoleucine (Ile) residues. Sensitivity was dramatically improved by 1H-detected 2D 1H/13C SSNMR by factors of 5.4-9.7 and 2.1-5.0, respectively, over 13C-detected 2D 1H/13C SSNMR and 1D 13C CPMAS, demonstrating that 2D 1H-detected SSNMR offers not only additional resolution but also sensitivity advantage over 1D 13C detection for the first time. High 1H resolution for the SAIL-labeled side-chain residues offered reasonable resolution even in the 2D data. A 1H-detected 3D 13C/13C/1H experiment on SAIL-ubiquitin provided nearly complete 1H and 13C assignments for seven Ile residues only within ~2.5 h. The results demonstrate the feasibility of side-chain signal assignment in this approach for as little as 10 nmol of a protein sample within ~3 days. The approach is likely applicable to a variety of proteins of biological interest without any requirements of highly efficient protein expression systems. 相似文献
994.
Hisanobu Ota Naofumi Takehara Tatsuya Aonuma Maki Kabara Motoki Matsuki Atsushi Yamauchi Toshiharu Takeuchi Jun-ichi Kawabe Naoyuki Hasebe 《PloS one》2015,10(4)
Objective
Relationship between microalbuminuria and worse outcome of coronary artery disease patients is discussed, but its underlying pathophysiological mechanism remains unclear. We investigated the role of microalbuminuria to the function of endothelial progenitor cells (EPCs), that might affect to outcome of acute myocardial infarction (AMI) patients.Methods
Forty-five AMI patients were divided into two groups according to their urinary albumin excretion: normal (n = 24) and microalbuminuria (>30 mg/day, n = 21). At day-2 and day-7 after AMI onset, circulating-EPCs (CD34+Flk1+) were quantified by flow cytometry. The number of lectin-acLDL-positive cultured-EPCs immobilized on fibronectin was determined. To assess the cellular senescence of cultured-EPCs, the expression level of sirtuin-1 mRNA and the number of SA-β-gal positive cell were evaluated. Angiographic late in-stent loss after percutaneous coronary intervention (PCI) was evaluated at a six-month follow-up.Results
No significant differences in coronary risk and the extent of myocardial damage were observed between the two groups. Late in-stent loss at the six-month follow-up was significantly higher in the microalbuminuria group (normal : microalbuminuria = 0.76±0.34 : 1.18±0.57 mm, p=0.021). The number of circulating-EPCs was significantly increased in microalbuminuria group at day-7, however, improved adhesion of EPCs was observed in normal group but not in microalbuminuria group from baseline to day-7 (+3.1±8.3 : -1.3±4.4 %: p<0.05). On the other hand, in microalbuminuria group at day-7, the level of sirtuin-1 mRNA expression of cultured-EPCs was significantly decreased (7.1±8.9 : 2.5±3.7 fold, p<0.05), which was based on the negative correlation between the level of sirtuin-1 mRNA expression and the extent of microalbuminuria. The ratio of SA-β-gal-positive cells in microalbuminuria group was increased compared to that of normal group.Conclusions
Microalbuminuria in AMI patients is closely associated with functional disorder of EPCs via cellular senescence, that predicts the aggravation of coronary remodeling after PCI. 相似文献995.
Yamauchi J Ishii N 《Journal of strength and conditioning research / National Strength & Conditioning Association》2007,21(3):703-709
Relations between force-velocity characteristics of the multijoint movement of the lower limbs and vertical jump performance were investigated. A total of 67 untrained subjects (age: 19.54 +/- 2.38 years; height: 166.88 +/- 8.53 cm; body mass: 59.14 +/- 10.82 kg, mean +/- SD) performed isometric and isotonic knee-hip extension movements on a servo-controlled dynamometer, and the force-velocity relations were determined. Also, vertical jump (VJ) performance was measured with a jump gauge. The force-velocity relation was described with a linear function so that the maximum isometric force (Fmax) and the maximum unloaded velocity (Vmax) for the knee-hip extension movement were estimated by extrapolation. Maximum isometric force coincided with maximum isometric force, F(0) (F(0)/Fmax = 1.03 +/- 0.24). Maximum isometric force, Vmax, and maximum power output (Pmax) were positively correlated with VJ (r = 0.48, 0.68, and 0.76, respectively; p < 0.001). However, when Fmax, Vmax, and Pmax were normalized with body mass (BM), leg length (LL), and BM, respectively, no correlation was seen between Fmax/BM and VJ (r = 0.24, p > 0.05), and significant correlations were seen between Vmax/LL and VJ (r = 0.56, p < 0.001) and between Pmax/BM and VJ (r = 0.65, p < 0.001). On the other hand, Fmax and Vmax (r = 0.12, p > 0.05) and Fmax/BM and Vmax/LL (r = 0.05, p > 0.05) were not significantly correlated, indicating that Fmax and Vmax were independent variables. The present estimates of Fmax, Vmax, and Pmax can be useful for evaluating the actual performance of multijoint movement of the lower limbs. It is suggested that, although in untrained individuals the speed of movement might be a more important determinant of jump performance, jump performance ability has a potential to improve with increases in strength of the lower limb. 相似文献
996.
997.
Yamauchi S Sugahara T Matsugi J Someya T Masuda T Kishida T Akiyama K Maruyama M 《Bioscience, biotechnology, and biochemistry》2007,71(9):2283-2290
The effect of the benzylic structure of lignan on antioxidant activity was evaluated. Secoisolariciresinol (1) and 3,4-bis(4-hydroxy-3-methoxybenzyl)tetrahydrofuran (2), which have two secondary benzylic positions without oxygen, showed the highest antioxidant activity. Optically active verrucosin (4) was synthesized for the first time in this experiment. 相似文献
998.
Haruo Hashimoto Toshiro Arai Yasuyuki Ohnishi Tomoo Eto Mamoru Ito Kyoji Hioki Ryo Suzuki Toshimasa Yamauchi Mitsuru Ohsugi Muneo Saito Yoshito Ueyama Kazuyuki Tobe Takashi Kadowaki Norikazu Tamaoki Kinori Kosaka 《Experimental Animals》2007,56(2):149-154
We studied the impact of "IVF - ET" on the glucose tolerance test (GTT), insulin tolerance test (ITT) and adiponectin to investigate differences in the phenotypes of B6J- Irs2(-/-) mice. The B6J-Irs2(-/-) mice (KO-Nat group) were prepared by natural mating. Other mice were produced by IVF-ET used ICR strain recipients and surrogate mothers (KO-IVF group). Measurement of body weight, GTT, ITT and blood sampling were performed at the ages of 6, 14 and 24 weeks after birth. Body weights, impaired glucose tolerance, insulin resistance and plasma adiponectin concentrations did not differ for each gender between the KO-IVF and KO-Nat groups. Therefore, we concluded that phenotypes of Irs2(-/-) mice produced by reproductive technology are stable. 相似文献
999.
1000.
Limviphuvadh V Tanaka S Goto S Ueda K Kanehisa M 《Bioinformatics (Oxford, England)》2007,23(16):2129-2138
MOTIVATION: Neurodegenerative disorders (NDDs) are progressive and fatal disorders, which are commonly characterized by the intracellular or extracellular presence of abnormal protein aggregates. The identification and verification of proteins interacting with causative gene products are effective ways to understand their physiological and pathological functions. The objective of this research is to better understand common molecular pathogenic mechanisms in NDDs by employing protein-protein interaction networks, the domain characteristics commonly identified in NDDs and correlation among NDDs based on domain information. RESULTS: By reviewing published literatures in PubMed, we created pathway maps in Kyoto Encyclopedia of Genes and Genomes (KEGG) for the protein-protein interactions in six NDDs: Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), Huntington's disease (HD), dentatorubral-pallidoluysian atrophy (DRPLA) and prion disease (PRION). We also collected data on 201 interacting proteins and 13 compounds with 282 interactions from the literature. We found 19 proteins common to these six NDDs. These common proteins were mainly involved in the apoptosis and MAPK signaling pathways. We expanded the interaction network by adding protein interaction data from the Human Protein Reference Database and gene expression data from the Human Gene Expression Index Database. We then carried out domain analysis on the extended network and found the characteristic domains, such as 14-3-3 protein, phosphotyrosine interaction domain and caspase domain, for the common proteins. Moreover, we found a relatively high correlation between AD, PD, HD and PRION, but not ALS or DRPLA, in terms of the protein domain distributions. AVAILABILITY: http://www.genome.jp/kegg/pathway/hsa/hsa01510.html (KEGG pathway maps for NDDs). 相似文献