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11.
H Suzuki Y Kondo A D Carlos J C Seidel 《The Journal of biological chemistry》1988,263(22):10974-10979
The effects of ionic strength, MgATP, and phosphorylation on the degradation rates of heavy meromyosin (HMM) by papain have been compared to their effects on the sedimentation coefficient (s20,w) to determine the relationship of the degradation rate to the equilibrium between the flexed and the extended forms (Suzuki, H., Stafford, W. F., Slayter, H. S., and Seidel, J. C. (1985) J. Biol. Chem. 260, 14810-14817). At 0.025 M NaCl, where HMM is predominantly in the flexed form, MgATP, Mg-adenylyl imidodiphosphate or MgADP reduce kH by 80-90%. MgATP exerts its optimal effect at this ionic strength, where at least 70% of HMM is flexed in the presence or absence of MgATP, suggesting that nucleotides reduce kH by decreasing the proteolytic susceptibility of the flexed form. At 0.5 M NaCl, where HMM is in the extended form, MgATP has no effect on kH. At low ionic strengths phosphorylation decreases kH but increases it in the presence of MgATP. Plots of kH against s20,w determined at various ionic strengths are linear, the data for phosphorylated and dephosphorylated HMM falling on the same line. Thus, raising the ionic strength or phosphorylating the 20-kDa light chain appears to alter kH by increasing the fraction of HMM in the extended form. The degradation rate of the 20-kDa light chain (kL) of dephosphorylated HMM responds to changes in ionic strength in essentially the same way as does kH, suggesting that the response of kL to changes in ionic strength can also be attributed to conversion of HMM to the extended form. However, kL for phosphorylated HMM measured in the presence of MgATP exhibits very little dependence on ionic strength. 相似文献
12.
Calcium ions produce a 3-4-fold stimulation of the actin-activated ATPase activities of phosphorylated myosin from bovine pulmonary artery or chicken gizzard at 37 degrees C and at physiological ionic strengths, 0.12-0.16 M. Actins from either chicken gizzard or rabbit skeletal muscle stimulate the activity of phosphorylated myosin in a Ca2+-dependent manner, indicating that the Ca2+ sensitivity involves myosin or a protein associated with it. Partial loss of Ca2+ sensitivity upon treatment of phosphorylated gizzard myosin with low concentrations of chymotrypsin and the lack of any change on similar treatment of actin supports the above conclusion. Although both actins enhance ATPase activity, activation by gizzard actin exhibits Ca2+ dependence at higher temperatures or lower ionic strengths than does activation by skeletal muscle actin. The Ca2+ dependence of the activity of phosphorylated heavy meromyosin is about half that of myosin and is affected differently by temperature, ionic strength and Mg2+, being independent of temperature and optimal at lower concentrations of NaCl. Raising the concentration of Mg2+ above 2-3 mM inhibits the activity of heavy meromyosin but stimulates that of myosin, indicating that Mg2+ and Ca2+ activate myosin at different binding sites. 相似文献
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The influences of a single rtg irradiation dose (14 mGy) on some quantitative signs in dependence on the body length was studied in the experimental fishes Barbus conchonius and B. tetrazona. The values of all followed signs increased positively due to the body length growth. The irradiation with the abovementioned dose in the given conditions had relative small influence on the growth course of the chosen quantitative sign values in the control and irradiated fishes of both species is the same (the regression lines are in good agreement, resp. they are parallel). The statistically significant difference was stated only between the irradiated the non-irradiated groups of B. tetrazona due to the head height (the regression lines are divergent). 相似文献
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Zusammenfassung Zellen des aus unreifen Plasmoblasten bestehenden, transplantablen KG 13-Plasmocytoms des Goldhamsters zeigen bei phasenoptischer Lebendbeobachtung in vitro ein besonders großes, stark strukturiertes Golgifeld. Dieses wird von einem Saum heller Pinocytosetröpfchen gegen die Speichergranula abgesetzt. Die Form des Golgifeldes, die Gesetzmäßigkeit seiner Veränderungen, die Spezifität seiner Strukturen und seine Beziehung zum Zellkern werden beschrieben. Pinocytosebläschen wandern bei dieser Zellart im gesamten Verlauf sichtbar von der Zellmembran zwischen den Speichergranula hindurch zur Randzone des Golgifeldes und lösen sich dort auf. Die Granula des Cytoplasmas entstehen offenbar im Golgifeld und werden in seiner Randzone erstmalig als solche erkennbar, um ins Cytoplasma zu wandern.
Die Arbeit wurde mit Unterstützung durch die Deutsche Forschungsgemeinschaft durchgeführt. 相似文献
Summary The KG 13 plasmocytoma from the Syrian golden hamster consists of immature Plasmoblasts. Living cells of this plasmocytoma observed by phase contrast microscopy in the hanging drop in vitro have a particularly large Golgi field surrounded by a border of light pinocytose bubbles. The cytoplasma is filled with storage granules. The form and the modifications of the Golgi field are described as well as his structure and his relations to the nucleus. Pinocytose bubbles reach the border of the Golgi field after crossing granules and disappeare. The granules are evidently formed in the Golgi field and become visible in its periphery.
Die Arbeit wurde mit Unterstützung durch die Deutsche Forschungsgemeinschaft durchgeführt. 相似文献
19.
Maria Reuter Margaretha K. S. Gustafsson Katja Mäntylä Cornelius J. P. Grimmelikhuijzen 《Zoomorphology》1996,116(3):111-122
The organization of the nervous system ofDendrocoelum lacteum (Tricladida, Paludicola, Dendrocelidae) andPolycelis tenuis (Tricladida, Paludicola, Planariidae) was studied by immunocytochemical double staining, using neuropeptide RFamide and serotonin
(5-HT) antisera on cryosections. The study confirmed the status of the main nerve cords (MCs) as the most important and stable
of the longitudinal cords and supported the hypothesis of a common phylogenetic origin of the MCs in flatworms. The ganglion-like
structures along the MCs at the beginning of transverse commissures and laterla branches showed a close contact with ventral
fibres of the submuscular nerve plexus indicating an origin from crossing points of insunken ring commissures. The distributional
pattern and morphology of the RFamide-IR cell bodies inD. lacteum corresponded to that of neurosecretory cells. Most RFamide-IR cells were unipolar and rounded while 5-HT-IR cells were uni-
bi- and multipolar. The neutropile consisted of a dense RFamide-IR and a loose 5-HT-IR network. RFamide dominated in all parts
of the genital plexus. 相似文献
20.
Significance of Ca2+ and K+ for the complex morphogenesis ofMicrasterias, which takes place through multipolar tip growth,was investigated. Studies with different external Ca2+ concentrationsand Ca2+ channel inhibitors LaCl3 and verapamil indicate thatCa2+ and Ca2+ channels are essential in the development, whiletreatments with different K+ concentrations and K+ channel inhibitorTEA demonstrate that potassium or K+ channels are not neededin the process, albeit the existence of K+ channels. K+ is notneeded even for the regulation of turgor pressure, which wasfound to decrease clearly during cell development. The plasmamembrane ATPase inhibitors diethylstilbesterol (DES) and Na-orthovanadatestop morphogenesis and indicate the importance of ion pumpsin the developmental process. Both supraoptimal, external K+and Ca2+ cause abundant Ca2+ precipitate formation in chloroplasts,which shows that chloroplasts are important in regulation ofcytoplasmic Ca2+ metabolism and that K+ activates the uptakeof Ca2+ through Ca2+ channels. (Received June 13, 1995; Accepted September 13, 1996) 相似文献