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Hepatocytes were isolated by collagenase perfusion of the liver from rainbow trout acclimated to 10 and 20 degrees C. The suitability of the stimulation of cellular respiration by succinate as criterion of viability was examined and discussed. Endogenous respiration rates of the hepatocytes were a function of cell size to the power of 0.8. Specific oxygen consumption of the hepatocytes and respiratory control ratios of the mitochondria in situ were independent of acclimation temperature. 相似文献
214.
Effects of temperature on glucose release and glycogen metabolism in isolated hepatocytes from rainbow trout (Salmo gairdneri) 总被引:1,自引:0,他引:1
Endogenous glucose release and glycogen metabolism were investigated in isolated hepatocytes from rainbow trout acclimated to 10 and 20 degrees C. Thermal acclimation did not significantly affect hepatocyte glycogen contents and the rates of glucose release during substrate-free incubations. In both acclimation groups glucose production and glycogen metabolism exhibited clearly different dependencies on assay temperature. It was concluded, that there are different sources of glucose release in the lower and upper temperature range--gluconeogenesis from endogenous precursors at low temperatures and glycogenolysis at high temperatures. This conclusion was supported by experiments with 3-mercaptopicolinic acid, which stimulated glycogen breakdown especially in the low temperature range. 相似文献
215.
William N. Norton Ierachmiel Daskal Howard E. Savage Richard A. Seibert Montague Lane 《Virchows Archiv. B, Cell pathology including molecular pathology》1979,30(1):43-51
Ultrastructural studies indicate that galactoflavin-induced riboflavin deficiency induces asynchrony of rat erythroblast maturation. During the latter stages of maturation erythroblasts retain significantly larger numbers of ribosomes as compared to control cells. Nucleoli are not evident in erythroblasts whose nuclei indicate cells in the latter stages of development. Membrane whorls develop within the mitochondria of plasma cells, eosinophils and neutrophils during the fifth week of riboflavin deficiency. No further evidence of degeneration was noted among additional cell organelles. 相似文献
216.
Plant Functional Types Differ in Their Long-term Nutrient Response to eCO2 in an Extensive Grassland
Seibert Ruben Andresen Louise C. Jarosch Klaus A. Moser Gerald Kammann Claudia I. Yuan Naiming Luterbacher Jrg Laughlin Ronnie J. Watson Catherine J. Erbs Martin Mller Christoph 《Ecosystems》2022,25(5):1084-1095
Ecosystems - Increasing atmospheric CO2 enhances plant biomass production and may thereby change nutrient concentrations in plant tissues. The objective of this study was to identify the effect of... 相似文献
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