全文获取类型
收费全文 | 2431篇 |
免费 | 183篇 |
国内免费 | 2篇 |
专业分类
2616篇 |
出版年
2023年 | 11篇 |
2022年 | 27篇 |
2021年 | 47篇 |
2020年 | 42篇 |
2019年 | 47篇 |
2018年 | 55篇 |
2017年 | 43篇 |
2016年 | 69篇 |
2015年 | 107篇 |
2014年 | 85篇 |
2013年 | 168篇 |
2012年 | 177篇 |
2011年 | 202篇 |
2010年 | 134篇 |
2009年 | 113篇 |
2008年 | 153篇 |
2007年 | 166篇 |
2006年 | 137篇 |
2005年 | 134篇 |
2004年 | 121篇 |
2003年 | 96篇 |
2002年 | 102篇 |
2001年 | 12篇 |
2000年 | 16篇 |
1999年 | 17篇 |
1998年 | 19篇 |
1997年 | 18篇 |
1996年 | 8篇 |
1995年 | 9篇 |
1994年 | 10篇 |
1993年 | 16篇 |
1992年 | 12篇 |
1991年 | 11篇 |
1990年 | 22篇 |
1989年 | 13篇 |
1988年 | 15篇 |
1987年 | 14篇 |
1986年 | 10篇 |
1985年 | 14篇 |
1984年 | 8篇 |
1982年 | 12篇 |
1981年 | 11篇 |
1979年 | 11篇 |
1978年 | 13篇 |
1976年 | 7篇 |
1975年 | 10篇 |
1974年 | 10篇 |
1973年 | 11篇 |
1972年 | 14篇 |
1969年 | 8篇 |
排序方式: 共有2616条查询结果,搜索用时 0 毫秒
61.
The recovery of radioactivity in the urine of guineapigs following a bolus intravenous dose of chromatographically pure 14C-Ntau-methylhistidine was measured in order to test whether the excretion of Ntau-methylhistidine (Ntau-MH) is a valid index of myofibrillar protein breakdown in these animals. Four male and four female guineapigs were dosed and after 7 days, 91.65+/-2.82% and 3.58+/-0.91% of injected radioactivity was recovered in the excreta and tissues, respectively. The average total recovery of 95.2+/-3.0% was not significantly different from 100%. Male guineapigs excreted the radioactivity more slowly than females (70% of the dose excreted within 74 h vs 39 h, respectively) but cumulative excretion at 7 days was the same for each sex. Chromatographic analysis of the urine showed almost all of the radioactivity to be associated with a single peak corresponding to Ntau-MH, indicating a lack of significant metabolism. These data show that although the clearance of 14C-Ntau-MH is slower than in rats or humans the urinary excretion of Ntau-MH is a valid index for myofibrillar protein degradation in the guineapig. 相似文献
62.
De Domenico I Nemeth E Nelson JM Phillips JD Ajioka RS Kay MS Kushner JP Ganz T Ward DM Kaplan J 《Cell metabolism》2008,8(2):146-156
Mammalian iron homeostasis is regulated by the interaction of the liver-produced peptide hepcidin and its receptor, the iron transporter ferroportin. Hepcidin binds to ferroportin resulting in degradation of ferroportin and decreased cellular iron export. We identify the hepcidin-binding domain (HBD) on ferroportin and show that a synthetic 19 amino acid peptide corresponding to the HBD recapitulates the characteristics and specificity of hepcidin binding to cell-surface ferroportin. The binding of mammalian hepcidin to ferroportin or the HBD shows an unusual temperature dependency with an increased rate of dissociation at temperatures below 15°C. The increased rate of dissociation is due to temperature- dependent changes in hepcidin structure. In contrast, hepcidin from poikilothermic vertebrates, such as fish or frogs, binds the HBD in a temperature-independent fashion. The affinity of hepcidin for the HBD permits a rapid, sensitive assay of hepcidin from all species and yields insights into the evolution of hepcidin. 相似文献
63.
64.
Cantanhede AM Da Silva VM Farias IP Hrbek T Lazzarini SM Alves-Gomes J 《Molecular ecology》2005,14(2):401-413
We used mitochondrial DNA control region sequences to examine phylogeography and population differentiation of the endangered Amazonian manatee Trichechus inunguis. We observe lack of molecular differentiation among localities and we find weak association between geographical and genetic distances. However, nested clade analysis supports restricted gene flow and/or dispersal with some long-distance dispersal. Although this species has a history of extensive hunting, genetic diversity and effective population sizes are relatively high when compared to the West Indian manatee Trichechus manatus. Patterns of mtDNA haplotype diversity in T. inunguis suggest a genetic disequilibrium most likely explained by demographic expansion resulting from secession of hunting and enforcement of conservation and protective measures. Phylogenetic analysis of T. manatus and T. inunguis haplotypes suggests that T. inunguis is nested within T. manatus, effectively making T. manatus a paraphyletic entity. Paraphyly of T. manatus and recent divergence times of T. inunguis and the three main T. manatus lineages suggest a possible need for a taxonomic re-evaluation of the western Atlantic Trichechus. 相似文献
65.
Future warmer seas: increased stress and susceptibility to grazing in seedlings of a marine habitat‐forming species 下载免费PDF全文
Gema Hernán María J. Ortega Alberto M. Gándara Inés Castejón Jorge Terrados Fiona Tomas 《Global Change Biology》2017,23(11):4530-4543
Increases in seawater temperature are expected to have negative consequences for marine organisms. Beyond individual effects, species‐specific differences in thermal tolerance are predicted to modify species interactions and increase the strength of top‐down effects, particularly in plant–herbivore interactions. Shifts in trophic interactions will be especially important when affecting habitat‐forming species such as seagrasses, as the consequences on their abundance will cascade throughout the food web. Seagrasses are a major component of coastal ecosystems offering important ecosystem services, but are threatened by multiple anthropogenic stressors, including warming. The mechanistic understanding of seagrass responses to warming at multiple scales of organization remains largely unexplored, especially in early‐life stages such as seedlings. Yet, these early‐life stages are critical for seagrass expansion processes and adaptation to climate change. In this study, we determined the effects of a 3 month experimental exposure to present and predicted mean summer SST of the Mediterranean Sea (25°C, 27°C, and 29°C) on the photophysiology, size, and ecology (i.e., plant‐herbivore interactions) of seedlings of the seagrass Posidonia oceanica. Warming resulted in increased mortality, leaf necrosis, and respiration as well as lower carbohydrate reserves in the seed, the main storage organ in seedlings. Aboveground biomass and root growth were also limited with warming, which could hamper seedling establishment success. Furthermore, warming increased the susceptibility to consumption by grazers, likely due to lower leaf fiber content and thickness. Our results indicate that warming will negatively affect seagrass seedlings through multiple direct and indirect pathways: increased stress, reduced establishment potential, lower storage of carbohydrate reserves, and increased susceptibly to consumption. This work provides a significant step forward in understanding the major mechanisms that will drive the capacity of seagrass seedlings to adapt and survive to warming, highlighting the potential additive effects that herbivory will have on ultimately determining seedling success. 相似文献
66.
Valéria Nogueira Machado Stuart C. Willis Aylton S. Teixeira Tomas Hrbek Izeni Pires Farias 《Environmental Biology of Fishes》2017,100(1):1-16
In the present study, sequences of the mtDNA control region (834 bp) were analyzed from 337 specimens of Prochilodus nigricans from sites along the main channel of the Amazonas River and three major tributaries, Madeira, Purus, and Juruá. The results of the analysis of molecular variance revealed that a large part of the genetic variation occurred within the populations analyzed (~85 %). Analysis with SAMOVA and Barriers suggested that the upper Madeira River and Purus Rivers had diverged genetically from the other samples, indicating restricted gene flow among these areas, while sites within the remaining range exhibited relatively little population structure. The high degree of structuring observed in the Madeira River basin population may be attributed to the presence of rapids along its upper course, while the genetic divergence found in the upper Purus River suggests historical connection between the upper Purus and upper Madeira Rivers followed by slow genetic drift due to large effective population sizes. However, given the life history and hypothesized evolutionary strategy of this species, we urge caution in interpreting that this targeted species is not at risk of overexploitation due to contemporary abundance. In order to preserve genetic diversity, we recommend enforcement of management regimes for regional stocks. 相似文献
67.
T Kirchhoff MM Gaudet AC Antoniou L McGuffog MK Humphreys AM Dunning SE Bojesen BG Nordestgaard H Flyger D Kang KY Yoo DY Noh SH Ahn T Dork P Schürmann JH Karstens P Hillemanns FJ Couch J Olson C Vachon X Wang A Cox I Brock G Elliott MW Reed B Burwinkel A Meindl H Brauch U Hamann YD Ko;GENICA Network A Broeks MK Schmidt LJ Van 't Veer LM Braaf N Johnson O Fletcher L Gibson J Peto C Turnbull S Seal A Renwick N Rahman PE Wu JC Yu CN Hsiung CY Shen MC Southey JL Hopper F Hammet T Van Dorpe 《PloS one》2012,7(6):e35706
Recently, a locus on chromosome 6q22.33 (rs2180341) was reported to be associated with increased breast cancer risk in the Ashkenazi Jewish (AJ) population, and this association was also observed in populations of non-AJ European ancestry. In the present study, we performed a large replication analysis of rs2180341 using data from 31,428 invasive breast cancer cases and 34,700 controls collected from 25 studies in the Breast Cancer Association Consortium (BCAC). In addition, we evaluated whether rs2180341 modifies breast cancer risk in 3,361 BRCA1 and 2,020 BRCA2 carriers from 11 centers in the Consortium of Investigators of Modifiers of BRCA1/2 (CIMBA). Based on the BCAC data from women of European ancestry, we found evidence for a weak association with breast cancer risk for rs2180341 (per-allele odds ratio (OR)?=?1.03, 95% CI 1.00-1.06, p?=?0.023). There was evidence for heterogeneity in the ORs among studies (I(2)?=?49.3%; p?=?<0.004). In CIMBA, we observed an inverse association with the minor allele of rs2180341 and breast cancer risk in BRCA1 mutation carriers (per-allele OR?=?0.89, 95%CI 0.80-1.00, p?=?0.048), indicating a potential protective effect of this allele. These data suggest that that 6q22.33 confers a weak effect on breast cancer risk. 相似文献
68.
69.
Dung beetle species interactions and multifunctionality are affected by an experimentally warmed climate 下载免费PDF全文
While substantial effort has been invested in modelling changes in species distribution with climate change, less attention has been given to how climate warming will affect interactions among co‐occurring species, and the cascading functional consequences. In this study, realistic dung beetle communities were subjected to an experimental warming treatment and the net effect on the functions of dung decomposition (in terms of dung mass) and plant productivity (in terms of biomass production of ryegrass grown on soil from underneath the dung pats) were examined. A priori, we hypothesized that the largest tunneling species would be functionally dominant, and be differently affected by experimental warming compared to pat‐dwelling, smaller species. In terms of dung decomposition, the largest beetles did prove to be the functionally most important, with the qualitative pattern unaffected by experimental warming. In contrast, for plant productivity all species appeared equally important under ambient conditions. However, the effects of single species on plant productivity were reduced as temperature increased: In a warmed climate, a combination of both tunneling and pat‐dwelling species came the closest to returning ecosystem functioning to levels found in the ambient treatment. These results suggest different roles for different species, and highlight the importance of maintaining multiple species within an ecosystem – particularly when systems are perturbed. 相似文献
70.
Darío C. Colautti Leandro Miranda Mariano Gonzalez-Castro Vanina Villanova Carlos A. Strüssmann Miguel Mancini Tomas Maiztegui Gustavo Berasain Ricardo Hattori Fabian Grosman Pablo Sanzano Ismael Lozano Sabina L. Vegh Victor Salinas Omar Del Ponti Pamela del Fresno Priscila Minotti Yoji Yamamoto Claudio R. M. Baigún 《Journal of fish biology》2020,96(1):202-216
In South America, the order Atheriniformes includes the monophyletic genus Odontesthes with 20 species that inhabit freshwater, estuarine and coastal environments. Pejerrey Odontesthes argentinensis is widely distributed in coastal and estuarine areas of the Atlantic Ocean and is known to foray into estuaries of river systems, particularly in conditions of elevated salinity. However, to our knowledge, a landlocked self-sustaining population has never been recorded. In this study, we examined the pejerrey population of Salada de Pedro Luro Lake (south-east of Buenos Aires Province, Argentina) to clarify its taxonomic identity. An integrative taxonomic analysis based on traditional meristic, landmark-based morphometrics and genetic techniques suggests that the Salada de Pedro Luro pejerrey population represents a novel case of physiological and morphological adaptation of a marine pejerrey species to a landlocked environment and emphasises the environmental plasticity of this group of fishes. 相似文献