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41.
Jose P. Vaqué Robert T. Dorsam Xiaodong Feng Ramiro Iglesias-Bartolome David J. Forsthoefel Qianming Chen Anne Debant Mark A. Seeger Bruce R. Ksander Hidemi Teramoto J. Silvio Gutkind 《Molecular cell》2013,49(1):94-108
Highlights? Human cancers harbor frequent mutations in Gq-linked GPCRs and Gαq subunits ? A genome-wide RNAi screen revealed that Trio is essential for activating AP-1 via Gαq ? A network of Trio-regulated Rho GTPases and MAPKs links Gq to the nucleus ? A hard-wired Gq-Trio signaling axis promotes the growth of many human malignancies 相似文献
42.
Aerobic bacteria, such as Burkholderia xenovorans LB400, are able to degrade a wide range of polychlorobiphenyls (PCBs). Generally, these bacteria are not able to transform
chlorobenzoates (CBAs), which accumulate during PCB degradation. In this study, the effects of CBAs on the growth, the morphology
and the proteome of Burkholderia
xenovorans LB400 were analysed. 4-CBA and 2-CBA were observed to inhibit the growth of strain LB400 on glucose. Strain LB400 exposed
to 4-CBA exhibited increased number and size of electron-dense granules in the cytoplasm, which could be polyphosphates. Two-dimensional
(2-D) polyacrylamide gel electrophoresis was used to characterise the molecular response of strain LB400 to 4-CBA. This compound
induced the enzymes BenD and CatA of benzoate and catechol catabolic pathways. The induction of molecular chaperones DnaK
and HtpG by 4-CBA indicated that the exposure to this compound constitutes a stressful condition for this bacterium. Additionally,
the induction of some Krebs cycle enzymes was observed, probably as response to cellular energy requirements. This study contributes
to the knowledge on the effects of CBA on the PCB-degrader Burkholderia xenovorans LB400. 相似文献
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Paul Gerhardt Seeger 《Cell and tissue research》1933,19(3):441-487
Ohne Zusammenfassung 相似文献
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We developed a fluorescence reader for the sensitive detection of surface-generated fluorescence. The system is applicable for high resolution imaging as well as for the readout of large biochips. The surface of a microscope coverslip is scanned with a laser beam focused to a waist diameter of 500 nm (FWHM) by means of a single aspheric lens. Scanning large areas with a focused beam usually evokes the need of automatic control elements to adjust the laser spot to the designated position at the surface. Due to the special design of the reader, the focus keeps at the plane of the surface even when scanning large areas, obviating the requirement of any real time control. Thus the instrument is straightforward and inexpensive. Nevertheless it features a high sensitivity and high optical resolution. The versatility of the instrument is demonstrated by imaging cells and reading out a DNA-chip. The excellent sensitivity is shown by detecting single fluorescently labeled antibodies. 相似文献
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Soni Pullamsetti Stefanie Krick Hüseyin Yilmaz Hossein Ardeschir Ghofrani Christian Schudt Norbert Weissmann Beate Fuchs Werner Seeger Friedrich Grimminger Ralph Theo Schermuly 《Respiratory research》2005,6(1):128