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61.
EMBO J
32
13, 1817–1828 doi:10.1038/emboj.2013.96; published online April262013During evolution, the mammalian brain massively expanded its size. However, the exact roles of distinct neural precursors, identified in the developing cortex during embryogenesis, for size expansion and surface folding (i.e., gyration) remain largely unknown. New findings by Nonaka-Kinoshita et al advance our understanding of embryonic neural precursor function by identifying cell type-selective functions for size expansion and folding, and challenge previously held concepts of mammalian brain development.Over the course of evolution, the mammalian brain massively expanded its size and complexity, which is believed to be responsible for an increase in cognitive functions and intellectual skills. The increase in brain size and number of cortical neurons is primarily due to an increased surface area by generating folds (gyrations) while the cortical thickness remained relatively constant (Lui et al, 2011). In the last decade, substantial progress has been made in identifying the cellular sources of cortex development. Using genetic lineage tracing of individual cell populations and time-lapse imaging of rodent and human slices of the embryonic cortex, radial glial cells (RGCs) were identified as the primary progenitors or neural stem cells (NSCs) in the developing cortex (Gotz and Huttner, 2005). Simplified, RG in the ventricular zone (VZ) line the ventricular surface and self-renew through symmetric divisions or give rise to basal progenitors (BPs; also called intermediate progenitors) in the subventricular zone (SVZ) that typically divide symmetrically and generate neurons. In contrast to the lissencephalic rodent brain, the developing cortex of gyrated mammals (e.g., humans and ferrets) contains a large number of basal radial glial (bRG) cells that reside in the outer subventricular zone (OSVZ), retain a cellular process that is connected to the pial surface and that are, in contrast to BPs, multipotent, meaning that they have the potency to generate diverse neural cell types (Fietz et al, 2010; Hansen et al, 2010; Reillo et al, 2011).Largely based on the anatomical differences between the developing cortex of lissencephalic and gyrencephalic brains, several hypotheses have been formulated aiming to explain the massive increase in size and induction of brain folding during mammalian evolution. One prominent hypothesis, called the radial unit hypothesis, suggests that the expansion of RGCs lining the ventricle leads to an increase of radial units that generate neurons and thus is responsible for the increase of surface area (Rakic, 1995). Others proposed that the increase in size and folding could be due to an increase in BP expansion in the SVZ compared to RGC numbers in the VZ, a hypothesis called the intermediate progenitor model (Kriegstein et al, 2006). These hypotheses were helpful to start explaining mammalian brain evolution, but testing the exact role of different neural precursors remained extremely challenging due to technical difficulties to selectively manipulating the proliferative activity of distinct precursor populations. Even though previous approaches were successful in enhancing brain size/neuron numbers in mouse models (e.g., by ectopically enhancing WNT signalling activity or manipulating the activity of the small RhoGTPase Cdc42 in neural precursors), these strategies had the drawback that the normal six-layered cortical topography was disrupted, making it difficult to draw definite conclusions (Chenn and Walsh, 2002; Cappello et al, 2006).In a collaborative work from the Calegari and Borrell laboratories, Nonaka-Kinoshita et al, 2013 now used an elegant approach to selectively enhance proliferation of distinct precursor populations in the mouse and ferret developing cortex. They used a previously described approach manipulating cell cycle length and subsequently proliferation by overexpressing the cell cycle regulators cdk4 and cyclinD1 that is sufficient to enhance neurogenesis without affecting cortical layering (a system called 4D) (Lange et al, 2009). For their mouse experiments, Nonaka-Kinoshita et al used a transgenic strategy to transiently overexpress 4D in nestin-expressing precursors using a tetracycline-controlled gene expression system (nestinrtTA/tetbi4D). With this approach, they selectively enhanced proliferation of BPs in the SVZ without affecting the number or proliferation of RGCs in the VZ (Nonaka-Kinoshita et al, 2013). Strikingly, targeted expansion of BPs induced a substantial increase in surface area but was not sufficient to induce cortical folding in the otherwise smooth mouse cortex, challenging the radial unit hypothesis and the intermediate progenitor model with regard to their predictions on the effects on size and/or gyration of the cortex upon expansion of the BP pool. Complementing their findings of BP expansion in the lissencephalic mouse brain, Nonaka-Kinoshita et al used retroviral vectors and electroporation of 4D expression constructs to target 4D expression to neural precursors in the developing ferret cortex that is gyrated under physiological conditions. In the ferret, 4D expression induced proliferation of multipotent bRG located in the OSVZ, as outlined above, a cell type that is found predominantly in gyrated cortices compared to lissencephalic brains. Notably, enhanced proliferation of bRG triggered the formation of novel cortical folds, suggesting that indeed the expansion of bRG may represent a key event during evolution to induce gyration and subsequent surface expansion of the mammalian brain (Borrell and Reillo, 2012; Nonaka-Kinoshita et al, 2013) (Figure 1). This now experimentally supported hypothesis is strongly reinforced by two recent publications: one from (Tuoc et al, 2013) who found that deletion of the chromatin remodelling protein BAF170 increases the BP pool and subsequently enhances brain size; and another one from the Götz laboratory where it was found that experimentally reduced expression levels of the DNA-associated protein Trnp1 substantially increased the expansion of bRG and BPs, inducing folding of the normally lissencephalic mouse brain (Stahl et al, 2013). Taken together, these studies suggest that bRG in the OSVZ play an important role in cortical folding by enhancing the generation of neurons and by providing a glial scaffold for newborn neurons to disperse more laterally and thus to form folds in the developing brain (Reillo et al, 2011).Open in a separate windowFigure 1How different neural precursors appear to regulate size expansion and folding during mammalian brain development. (A) Shown are the main cellular components of the cortex of the lissencephalic mouse brain during embryonic development with RGCs (blue) lining the lateral ventricles in the VZ that generate BPs (yellow) in the SVZ and provide a scaffold for migrating neurons (left; green). Note that the mouse developing brain contains only a few bRG in the OSVZ (red). Notably, expansion of BPs using the 4D strategy developed in the Calegari laboratory increases surface area of the murine cortex without inducing the folding of the smooth mouse brain surface (right panel). (B) In contrast to lissencephalic animals, the developing cortices of species with gyrated brains (e.g., humans and ferrets) contain a substantial number of bRG located in the OSVZ (left panel). 4D-based, virus-mediated expansion of bRG in the ferret cortex leads to the induction of additional folds in the ferret cortex, indicating that the proliferative activity of bRG is critically involved in the extent of folding in physiologically gyrated brains (right panel).Even though this new study challenges previously held concepts regarding size expansion and folding of the mammalian brain, future studies are required that even more selectively enhance the proliferation and expansion of distinct precursor subtypes with high temporal and spatial control. Thus, the combination of sophisticated genetic tools to enhance precursor activity with detailed molecular analyses (e.g., analysing gene expression in highly folded versus unfolded brain regions, an approach that already showed differential levels of Trnp1 expression; Stahl et al, 2013) and live-imaging studies in the developing mammalian cortex will further enhance the understanding how our brains developed during evolution. 相似文献
62.
S.P. Meenakshisundaram B. Karthikeyan K. Muthu S. Sebastian 《Molecular simulation》2013,39(7):584-595
The experimental and theoretical studies on the molecular structure and vibrational spectra of bis(thiourea)zinc(II) chloride (BTZC) crystals were investigated. The Fourier transform infrared, Fourier transform Raman and UV–vis spectra of BTZC were recorded. The molecular geometry and vibrational frequencies of BTZC in the ground state were calculated by using B3LYP with LANL2DZ as basis set. Comparison of the observed structural parameters of BTZC with single-crystal X-ray studies yields a good agreement. Vibrational analysis of the simultaneous IR and Raman activation of the Zn–Cl stretching mode in the molecule provides the evidence for the charge transfer interaction taking place within the molecule. The energy and oscillator strength are calculated by time-dependent density functional theory. The simulated spectra satisfactorily coincide with the experimental spectra. 相似文献
63.
64.
Felix Willmund Marta del Alamo Sebastian Pechmann Taotao Chen Véronique Albanèse Eric B. Dammer Junmin Peng Judith Frydman 《Cell》2013,152(1-2):196-209
Highlights? Yeast Hsp70 SSB binds cotranslationally to a defined set of nascent polypeptides ? SSB shows specificity for substrates highly challenged in cotranslational folding ? The heterodimeric cochaperone RAC modulates cotranslational SSB specificity ? Loss of SSB function leads to widespread misfolding and aggregation 相似文献
65.
Frank O. Aylward Bradon R. McDonald Sandra M. Adams Alejandra Valenzuela Rebeccah A. Schmidt Lynne A. Goodwin Tanja Woyke Cameron R. Currie Garret Suen Michael Poulsen 《Applied and environmental microbiology》2013,79(12):3724-3733
Sphingomonads comprise a physiologically versatile group within the Alphaproteobacteria that includes strains of interest for biotechnology, human health, and environmental nutrient cycling. In this study, we compared 26 sphingomonad genome sequences to gain insight into their ecology, metabolic versatility, and environmental adaptations. Our multilocus phylogenetic and average amino acid identity (AAI) analyses confirm that Sphingomonas, Sphingobium, Sphingopyxis, and Novosphingobium are well-resolved monophyletic groups with the exception of Sphingomonas sp. strain SKA58, which we propose belongs to the genus Sphingobium. Our pan-genomic analysis of sphingomonads reveals numerous species-specific open reading frames (ORFs) but few signatures of genus-specific cores. The organization and coding potential of the sphingomonad genomes appear to be highly variable, and plasmid-mediated gene transfer and chromosome-plasmid recombination, together with prophage- and transposon-mediated rearrangements, appear to play prominent roles in the genome evolution of this group. We find that many of the sphingomonad genomes encode numerous oxygenases and glycoside hydrolases, which are likely responsible for their ability to degrade various recalcitrant aromatic compounds and polysaccharides, respectively. Many of these enzymes are encoded on megaplasmids, suggesting that they may be readily transferred between species. We also identified enzymes putatively used for the catabolism of sulfonate and nitroaromatic compounds in many of the genomes, suggesting that plant-based compounds or chemical contaminants may be sources of nitrogen and sulfur. Many of these sphingomonads appear to be adapted to oligotrophic environments, but several contain genomic features indicative of host associations. Our work provides a basis for understanding the ecological strategies employed by sphingomonads and their role in environmental nutrient cycling. 相似文献
66.
Nadine Flinner Lars Ellenrieder Sebastian B. Stiller Thomas Becker Enrico Schleiff Oliver Mirus 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》2013,1833(12):3314-3325
Mitochondrial β-barrel proteins fulfill central functions in the outer membrane like metabolite exchange catalyzed by the voltage-dependent anion channel (VDAC) and protein biogenesis by the central components of the preprotein translocase of the outer membrane (Tom40) or of the sorting and assembly machinery (Sam50). The mitochondrial division and morphology protein Mdm10 is another essential outer membrane protein with proposed β-barrel fold, which has so far only been found in Fungi. Mdm10 is part of the endoplasmic reticulum mitochondria encounter structure (ERMES), which tethers the ER to mitochondria and associates with the SAM complex. In here, we provide evidence that Mdm10 phylogenetically belongs to the VDAC/Tom40 superfamily. Contrary to Tom40 and VDAC, Mdm10 exposes long loops towards both sides of the membrane. Analyses of single loop deletion mutants of Mdm10 in the yeast Saccharomyces cerevisiae reveal that the loops are dispensable for Mdm10 function. Sequences similar to fungal Mdm10 can be found in species from Excavates to Fungi, but neither in Metazoa nor in plants. Strikingly, the presence of Mdm10 coincides with the appearance of the other ERMES components. Mdm10's presence in both unikonts and bikonts indicates an introduction at an early time point in eukaryotic evolution. 相似文献
67.
68.
69.
Robert Gliniars Gabriel Sebastian Becker Désirée Braun Helmut Dalitz 《Trees - Structure and Function》2013,27(4):1129-1138
Monthly stem increment of 766 trees was assessed for 7 years in Kakamega Forest, Kenya and related to monthly climatic variables. Mean stem increment of all tree individuals correlated negatively with maximum temperature but not with mean and minimum temperatures. For the precipitation variables sum of precipitation and number of rainy days we found positive correlations. Stem increment of the trees in the early-, mid-, and late-successional groups correlated positively with the number of rainy days. For late-successional trees increment correlated negatively with mean and maximum temperature and positively with all other precipitation variables. For mid-successional trees we found a negative correlation with mean temperature. In addition, the stem increment of most species related positively to precipitation variables and negatively to mean and maximum temperature. In view of the expected increasing temperatures and fewer but heavier rain events, our results suggest that climate change will lead to a reduction in stem increment. The results appertaining to the successional groups imply that early and mid-successional species are better equipped to perform well under the expected future climatic conditions than the late-successional species. This could reduce the role of this East African forest as a carbon store. As the responses to climatic variables were highly group- and species-specific it is likely that climate change will result in a species composition shift, presumably in favour of drought-resistant and heat-tolerating species. 相似文献
70.
Sebastian Tarcz 《European journal of protistology》2013,49(1):50-61
Paramecium novaurelia Beale and Schneller, 1954, was first found in Scotland and is known to occur mainly in Europe, where it is the most common species of the P. aurelia complex. In recent years, two non-European localities have been described: Turkey and the United States of America. This article presents the analysis of intraspecific variability among 25 strains of P. novaurelia with the application of ribosomal and mitochondrial loci (ITS1-5.8S-ITS2, 5′ large subunit rDNA (5′LSU rDNA) and cytochrome c oxidase subunit 1 (COI) mtDNA). The mean distance observed for all of the studied P. novaurelia sequence pairs was p = 0.008/0.016/0.092 (ITS1-5.8S-ITS2/5′LSU rDNA/COI). Phylogenetic trees (NJ/MP/BI) based on a comparison of all of the analysed sequences show that the studied strains of P. novaurelia form a distinct clade, separate from the P. caudatum outgroup, and are divided into two clusters (A and B) and two branches (C and D). The occurrence of substantial genetic differentiation within P. novaurelia, confirmed by the analysed DNA fragments, indicates a rapid evolution of particular species within the Paramecium genus. 相似文献