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31.
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Mateusz Kurcinski Aleksandra Badaczewska‐Dawid Michal Kolinski Andrzej Kolinski Sebastian Kmiecik 《Protein science : a publication of the Protein Society》2020,29(1):211-222
Molecular docking of peptides to proteins can be a useful tool in the exploration of the possible peptide binding sites and poses. CABS‐dock is a method for protein–peptide docking that features significant conformational flexibility of both the peptide and the protein molecules during the peptide search for a binding site. The CABS‐dock has been made available as a web server and a standalone package. The web server is an easy to use tool with a simple web interface. The standalone package is a command‐line program dedicated to professional users. It offers a number of advanced features, analysis tools and support for large‐sized systems. In this article, we outline the current status of the CABS‐dock method, its recent developments, applications, and challenges ahead. 相似文献
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Mycopathologia - Dermatophytosis is a widespread disease with high prevalence and a substantial economic burden associated with costs of treatment. The pattern of this infectious disease covers a... 相似文献
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Adem Yildirim Sina Mozaffari-Jovin Ann-Kathrin Wallisch Jessica Schfer Sebastian E J Ludwig Henning Urlaub Reinhard Lührmann Uwe Wolfrum 《Nucleic acids research》2021,49(10):5845
Splicing is catalyzed by the spliceosome, a compositionally dynamic complex assembled stepwise on pre-mRNA. We reveal links between splicing machinery components and the intrinsically disordered ciliopathy protein SANS. Pathogenic mutations in SANS/USH1G lead to Usher syndrome—the most common cause of deaf-blindness. Previously, SANS was shown to function only in the cytosol and primary cilia. Here, we have uncovered molecular links between SANS and pre-mRNA splicing catalyzed by the spliceosome in the nucleus. We show that SANS is found in Cajal bodies and nuclear speckles, where it interacts with components of spliceosomal sub-complexes such as SF3B1 and the large splicing cofactor SON but also with PRPFs and snRNAs related to the tri-snRNP complex. SANS is required for the transfer of tri-snRNPs between Cajal bodies and nuclear speckles for spliceosome assembly and may also participate in snRNP recycling back to Cajal bodies. SANS depletion alters the kinetics of spliceosome assembly, leading to accumulation of complex A. SANS deficiency and USH1G pathogenic mutations affects splicing of genes related to cell proliferation and human Usher syndrome. Thus, we provide the first evidence that splicing dysregulation may participate in the pathophysiology of Usher syndrome. 相似文献
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Genetica - The vertebrate mitochondrial genome is characterized by an exceptional organization evolving towards a reduced size. However, the persistence of a non-coding and highly variable control... 相似文献
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Chiara Stronczek Stephan Lange Belinda Bullard Sebastian Wolniak Emma Brgeson Olga Mayans Jennifer R. Fleming 《The Journal of general physiology》2021,153(7)
The N2A segment of titin is a main signaling hub in the sarcomeric I-band that recruits various signaling factors and processing enzymes. It has also been proposed to play a role in force production through its Ca2+-regulated association with actin. However, the molecular basis by which N2A performs these functions selectively within the repetitive and extensive titin chain remains poorly understood. Here, we analyze the structure of N2A components and their association with F-actin. Specifically, we characterized the structure of its Ig domains by elucidating the atomic structure of the I81-I83 tandem using x-ray crystallography and computing a homology model for I80. Structural data revealed these domains to present heterogeneous and divergent Ig folds, where I81 and I83 have unique loop structures. Notably, the I81-I83 tandem has a distinct rotational chain arrangement that confers it a unique multi-domain topography. However, we could not identify specific Ca2+-binding sites in these Ig domains, nor evidence of the association of titin N2A components with F-actin in transfected C2C12 myoblasts or C2C12-derived myotubes. In addition, F-actin cosedimentation assays failed to reveal binding to N2A. We conclude that N2A has a unique architecture that predictably supports its selective recruitment of binding partners in signaling, but that its mechanical role through interaction with F-actin awaits validation. 相似文献
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Ferencz Sandor Toth Denes Kaszas Balint Bardosi Sebastian Vicena Viktoria Karadi Oszkar Reglodi Dora Kelemen Dezso 《International journal of peptide research and therapeutics》2021,27(3):1719-1728
International Journal of Peptide Research and Therapeutics - Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with widespread occurrence and diverse functions. PACAP... 相似文献
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Alshareef Ahmed Wu Taotao Giudice J. Sebastian Panzer Matthew B. 《Biomechanics and modeling in mechanobiology》2021,20(6):2301-2317
Biomechanics and Modeling in Mechanobiology - Computational models of the brain have become the gold standard in biomechanics to understand, predict, and mitigate traumatic brain injuries. Many... 相似文献