全文获取类型
收费全文 | 103490篇 |
免费 | 1493篇 |
国内免费 | 887篇 |
专业分类
105870篇 |
出版年
2023年 | 74篇 |
2022年 | 132篇 |
2021年 | 291篇 |
2020年 | 140篇 |
2019年 | 196篇 |
2018年 | 12015篇 |
2017年 | 10833篇 |
2016年 | 7730篇 |
2015年 | 1105篇 |
2014年 | 855篇 |
2013年 | 927篇 |
2012年 | 4997篇 |
2011年 | 13530篇 |
2010年 | 12412篇 |
2009年 | 8557篇 |
2008年 | 10268篇 |
2007年 | 11839篇 |
2006年 | 687篇 |
2005年 | 909篇 |
2004年 | 1344篇 |
2003年 | 1392篇 |
2002年 | 1142篇 |
2001年 | 473篇 |
2000年 | 400篇 |
1999年 | 233篇 |
1998年 | 118篇 |
1997年 | 116篇 |
1996年 | 95篇 |
1995年 | 64篇 |
1994年 | 90篇 |
1993年 | 100篇 |
1992年 | 171篇 |
1991年 | 162篇 |
1990年 | 136篇 |
1989年 | 123篇 |
1988年 | 128篇 |
1987年 | 120篇 |
1986年 | 89篇 |
1985年 | 100篇 |
1984年 | 84篇 |
1983年 | 81篇 |
1982年 | 63篇 |
1981年 | 72篇 |
1980年 | 72篇 |
1979年 | 84篇 |
1977年 | 65篇 |
1975年 | 76篇 |
1974年 | 71篇 |
1972年 | 295篇 |
1971年 | 319篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
81.
The combination of two-dimensional mini gel electrophoresis with the 'western blot' technique proves to be a powerful tool in characterizing lymphoid cells. By testing for kappa and lambda immunoglobulin light chains we were able to differentiate between mono-, oligo-, and polyclonal B-cell diseases. The distribution of the lambda isotypes of 24 cases with chronic lymphocytic B-cell leukemia (B-CLL) tested was not random when compared to the distribution of the lambda light chains in B-lymphocytes of normal persons. This might implicate a genetic link between the lambda loci (chromosome 22) and the development of the lambda-CLL. 相似文献
82.
Organization of the ribosomal ribonucleic acid genes in various wild-type strains and wild-collected strains of Neurospora 总被引:6,自引:0,他引:6
Peter J. Russell Sheryl Wagner Karin D. Rodland Rhonda L. Feinbaum Jennifer P. Russell Marion S. Bret-Harte Stephen J. Free Robert L. Metzenberg 《Molecular & general genetics : MGG》1984,196(2):275-282
Summary The organization of the ribosomal DNA (rDNA) repcat unit in the standard wild-type strain of Neurospora crassa, 74-OR23-1A, and in 30 other wild-type strains and wild-collected strains of N. crassa, N. tetrasperma, N. sitophila, N. intermedia, and N. discreta isolated from nature, was investigated by restriction enzyme digestion of genomic DNA, and probing of the Southern-blotted DNA fragments with specific cloned pieces of the rDNA unit from 74-OR23-1A. The size of the rDNA unit in 74-OR23-1A was shown to be 9.20 kilobase pairs (kb) from blotting data, and the average for all strains was 9.11+0.21 kb; standard error=0.038; coefficient of variation (C.V.)=2.34%. These data indicate that the rDNA repeat unit size has been highly conserved among the Neurospora strains investigated. However, while all strains have a conserved HindIII site near the 5 end of the 25 S rDNA coding sequence, a polymorphism in the number and/or position of HindIII sites in the nontranscribed spacer region was found between strains. The 74-OR23-1A strain has two HindIII sites in the spacer, while others have from 0 to at least 3. This restriction site polymorphism is strain-specific and not species-specific. It was confirmed for some strains by restriction analysis of clones containing most of the rDNA repeat unit. The current restriction map of the 74-OR23-1A rDNA repeat unit is presented. 相似文献
83.
The defining equations of the multiple inert gas elimination technique are underdetermined, and an infinite number of VA/Q ratio distributions exists that fit the same inert gas data. Conventional least-squares analysis with enforced smoothing chooses a single member of this infinite family whose features are assumed to be representative of the family as a whole. To test this assumption, the average of all ventilation-perfusion ratio (VA/Q) distributions that are compatible with given data was calculated using a linear program. The average distribution so obtained was then compared with that recovered using enforced smoothing. Six typical sets of inert gas data were studied. In all sets but one, the distribution recovered with conventional enforced smoothing closely matched the structure of the average distribution. The single exception was associated with the broad log-normal VA/Q distribution, which is rarely observed using the technique. We conclude that the VA/Q distribution conventionally recovered approximates a simple average of all compatible distributions. It therefore displays average features and only that degree of fine structural detail that is typical of the family as a whole. 相似文献
84.
85.
The kinetics of haemolysis of rabbit erythrocytes byCroton tiglium lectin was studied as a function of concentration of the lectin and erythrocytes. The length of the prelytic period decreased with increasing lectin concentrations, indicating that the secondary events at the membrane which follow the binding of the lectin to cell surface carbohydrate receptors are accelerated at higher surface concentrations of the lectin. The rate or extent of haemolysis was not affected by the inclusion of ions like K+, Ca2+ and Mg2+ in the medium or by the substitution of ionic medium by a non-ionic medium. The inhibition of haemagglutination and haemolysis of rabbit red cells byCroton tiglium lectin by antilectin rabbit serum was observed. A possible mechanism of haemolysis by the lectin is discussed. 相似文献
86.
N-Acetyl-D-galactosamine in β-linkage being ubiquitous in cell surface glycoproteins, their interaction with lectins specific for this sugar moiety may be a significant event in cell adhesion phenomena. This article discusses the common β-N-acetyl galactosamine-specific lectins, with particular stress on the lectin from winged beans (Psophocarpus tetragonolobus). 相似文献
87.
88.
Demonstration of an “Excitation-Contraction Recoupling” Mechanism in Mammalian Ventricular Myocardium 总被引:1,自引:0,他引:1
A PROCESS called “excitation-contraction coupling” has been generally accepted to take place only in the direction of excitation to contraction. Through this mechanism a propagated action potential initiates an active state in skeletal or cardiac muscle and the muscle contracts. We propose that, in the mammalian ventricular myocardium at least, the process is not unidirectional and an important reverse coupling between the contractile system and the excitable plasma membrane has been overlooked. Through this feedback interaction the mode of contraction (that is, isotonic or isometric) not only determines the instantaneous electrical state of the plasma membrane, but also influences the mechanical events of the subsequent beats. Thus when Kaufmann et al.1 recorded intracellular action potentials from cat papillary muscle, the time course of the repolarization was altered depending on the mode of contraction. Some kind of contraction-excitation feedback has also been suggested by Stauch2 and Lab3,4. They showed a difference in the shape of the monophasic action potential, as recorded by a suction electrode, when comparing isotonic and isovolumic contraction of the intact ventricle. But their experimental conditions did not allow satisfactory analysis of the phenomenon. 相似文献
89.
90.
Function of the <Emphasis Type="Italic">rel</Emphasis> Gene in <Emphasis Type="Italic">Escherichia coli</Emphasis> 总被引:6,自引:0,他引:6
Sokawa et al. suggest that rel- strains of Escherichia coli possess abnormal protein synthesizing machinery, which cannot carry out normal protein synthesis when the supply of amino-acids is limited. 相似文献