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香蕉束顶病毒基因Ⅰ的克隆及序列分析 总被引:5,自引:0,他引:5
以中国漳州地区感染BBTV的香蕉组织总DNA为模板,根据我国台湾地区BBTV分离物基因组Ⅰ序列,设计并合成了一对引物,通过PCR扩增出约500bp的片段。利用pBluescriptⅡSK T-载体获得此片段的克隆,经测序表明为BBTV组分Ⅰ的部分序列。由已测知的BBTV基因组Ⅰ序列设计一对相邻引物,以我国漳州的感染BBTV香蕉组织总DNA为模板,通过PCR坟增出约1.1kb的片段。利用pBlues 相似文献
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D R Seals J M Hagberg W K Allen B F Hurley G P Dalsky A A Ehsani J O Holloszy 《Journal of applied physiology (Bethesda, Md. : 1985)》1984,56(6):1521-1525
Groups of endurance-trained masters athletes (60 +/- 2 yr), older untrained men (62 +/- 1 yr), lean older untrained men (61 +/- 2 yr), endurance-trained young athletes (26 +/- 1 yr), and young untrained men (28 +/- 1 yr) were studied to obtain information on the separate effects of age, physical activity, and body fatness on glucose tolerance and insulin sensitivity. Each subject underwent an oral 100-g glucose tolerance test. Skinfold thickness was determined at six sites. The trained groups had a higher maximum O2 uptake capacity and lower sum of skinfolds than their sedentary peers. The lean older untrained group had a sum of skinfolds similar to that of the young untrained group. The masters athletes, young athletes, and young untrained men exhibited similar glucose tolerance whereas the two older untrained groups had an almost twofold greater total area under the glucose curve (P less than 0.05). The masters and young athletes had significantly blunted plasma insulin responses compared with the other three groups (P less than 0.05). The young and the lean older untrained groups had similar plasma insulin responses with significantly lower insulin levels than the older untrained group (P less than 0.05). These results provide evidence that regularly performed vigorous exercise can, in some individuals, prevent the deterioration of glucose tolerance and insulin sensitivity with age. 相似文献
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Synaptosome Antisera affect Permeability of Synaptosomal Membranes <Emphasis Type="Italic">in vitro</Emphasis> 总被引:2,自引:0,他引:2
ANTISERA against specific regions of the brain can specifically influence the electrical activity of those regions1,2. It is possible that these immunological techniques may be used to determine the role of specific chemical components in brain function. 相似文献
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The neurohypophysial hormone, arginine vasotocin, is depletedfrom the hypothalamus, and rises in concentration in the bloodduring oviposition in hens. The contractile responses of isolatedoviducts from birds, reptiles and amphibians are more sensitiveto arginine vasotocin than to oxytocin or mesotocin. This evidenceclearly indicates that arginine vasotocin is involved in parturitionor oviposition in nonmammalian tetrapods. Evidence for a physiologicalrole for specific neurohypophysial hormones in the regulationof oviductor in some cases ovarian contractilityin fishes is unclear and occasionally contradictory. However,it appears unlikely that arginine vasotocin is involved in thefish species that have been investigated. It is evident that,much like the neurohypophysial hormones, the neurohypophysialhormone receptors of the vertebrate myometrium have undergoneevolutionary change. 相似文献
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