全文获取类型
收费全文 | 68篇 |
免费 | 9篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 3篇 |
2014年 | 1篇 |
2013年 | 4篇 |
2012年 | 5篇 |
2011年 | 1篇 |
2010年 | 7篇 |
2009年 | 5篇 |
2008年 | 3篇 |
2007年 | 4篇 |
2006年 | 7篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 1篇 |
2002年 | 1篇 |
2001年 | 2篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1983年 | 1篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1972年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有77条查询结果,搜索用时 135 毫秒
61.
Zea systematics: ribosomal ITS evidence 总被引:10,自引:0,他引:10
Ribosomal internal transcribed spacer (ITS) sequences were used to evaluate
the phylogenetics of Zea and Tripsacum. Maximum likelihood and polymorphism
parsimony were used for phylogenetic reconstructions. Zea ITS nucleotide
diversity was high compared to other plant species, but approximately
equivalent to other maize loci. Coalescence of ITS alleles was rapid
relative to other nuclear loci; however, there was still much diversity
within populations. Zea and Tripsacum form a clade clearly differentiated
from all other Poaceae. Four Zea ITS pseudogenes were identified by
phylogenetic position and nucleotide composition. The phylogenetic position
of Z. mays ssp. huehuetenangensis was clearly established as basal to the
other Z. mays. The ITS phylogeny disfavored a Z. luxurians and Z.
diploperennis clade, which conflicted with some previous studies. The
introgression of Z. mays alleles into Z. perennis and Z. diploperennis was
also established. The ITS data indicated a near contemporary divergence of
domesticated maize and its two closest wild relatives.
相似文献
62.
R. J. Safran E. S. C. Scordato M. R. Wilkins J. K. Hubbard B. R. Jenkins T. Albrecht S. M. Flaxman H. Karaardıç Y. Vortman A. Lotem P. Nosil P. Pap S. Shen S.‐F. Chan T.L. Parchman N. C. Kane 《Molecular ecology》2016,25(16):3865-3883
Population divergence in geographic isolation is due to a combination of factors. Natural and sexual selection may be important in shaping patterns of population differentiation, a pattern referred to as ‘isolation by adaptation’ (IBA). IBA can be complementary to the well‐known pattern of ‘isolation by distance’ (IBD), in which the divergence of closely related populations (via any evolutionary process) is associated with geographic isolation. The barn swallow Hirundo rustica complex comprises six closely related subspecies, where divergent sexual selection is associated with phenotypic differentiation among allopatric populations. To investigate the relative contributions of selection and geographic distance to genome‐wide differentiation, we compared genotypic and phenotypic variation from 350 barn swallows sampled across eight populations (28 pairwise comparisons) from four different subspecies. We report a draft whole‐genome sequence for H. rustica, to which we aligned a set of 9493 single nucleotide polymorphisms (SNPs). Using statistical approaches to control for spatial autocorrelation of phenotypic variables and geographic distance, we find that divergence in traits related to migratory behaviour and sexual signalling, as well as geographic distance, together explain over 70% of genome‐wide divergence among populations. Controlling for IBD, we find 42% of genomewide divergence is attributable to IBA through pairwise differences in traits related to migratory behaviour and sexual signalling alone. By (i) combining these results with prior studies of how selection shapes morphological differentiation and (ii) accounting for spatial autocorrelation, we infer that morphological adaptation plays a large role in shaping population‐level differentiation in this group of closely related populations. 相似文献
63.
64.
65.
66.
Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery 总被引:7,自引:2,他引:5
下载免费PDF全文
![点击此处可从《Biophysical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Multiphoton fluorescence photobleaching recovery (MP-FPR) is a technique for measuring the three-dimensional (3D) mobility of fluorescent molecules with 3D spatial resolution of a few microns. A brief, intense flash of mode-locked laser light pulses excites fluorescent molecules via multiphoton excitation in an ellipsoidal focal volume and photobleaches a fraction. Because multiphoton excitation of fluorophores is intrinsically confined to the high-intensity focal volume of the illuminating beam, the bleached region is restricted to a known, three-dimensionally defined volume. Fluorescence in this focal volume is measured with multiphoton excitation, using the attenuated laser beam to measure fluorescence recovery as fresh unbleached dye diffuses in. The time course of the fluorescence recovery signal after photobleaching can be analyzed to determine the diffusion coefficient of the fluorescent species. The mathematical formulas used to fit MP-FPR recovery curves and the techniques needed to properly utilize them to acquire the diffusion coefficients of fluorescently labeled molecules within cells are presented here. MP-FPR is demonstrated on calcein in RBL-2H3 cells, using an anomalous subdiffusion model, as well as in aqueous solutions of wild-type green fluorescent protein, yielding a diffusion coefficient of 8.7 x 10(-7) cm(2)s(-1) in excellent agreement with the results of other techniques. 相似文献
67.
68.
Coffee (Coffea arabica L.) genes early expressed during infection by the rust fungus (Hemileia vastatrix) 总被引:2,自引:0,他引:2
69.
ISABEL FERNANDES CLÁUDIA PASCOAL HELENA GUIMARÃES RUTE PINTO INÊS SOUSA FERNANDA CÁSSIO 《Freshwater Biology》2012,57(11):2306-2317
1. We investigated the effects of riparian plant diversity (species number and identity) and temperature on microbially mediated leaf decomposition by assessing fungal biodiversity, fungal reproduction and leaf mass loss. 2. Leaves of five riparian plant species were first immersed in a stream to allow microbial colonisation and were then exposed, alone or in all possible combinations, at 16 or 24 °C in laboratory microcosms. 3. Fungal biodiversity was reduced by temperature but was not affected by litter diversity. Temperature altered fungal community composition with species of warmer climate, such as Lunulospora curvula, becoming dominant. 4. Fungal reproduction was affected by litter diversity, but not by temperature. Fungal reproduction in leaf mixtures did not differ or was lower than that expected from the weighted sum of fungal sporulation on individual leaf species. At the higher temperature, the negative effect of litter diversity on fungal reproduction decreased with the number of leaf species. 5. Leaf mass loss was affected by the identity of leaf mixtures (i.e. litter quality), but not by leaf species number. This was mainly explained by the negative correlation between leaf decomposition and initial lignin concentration of leaves. 6. At 24 °C, the negative effects of lignin on microbially mediated leaf decomposition diminished, suggesting that higher temperatures may weaken the effects of litter quality on plant litter decomposition in streams. 7. The reduction in the negative effects of lignin at the higher temperature resulted in an increased microbially mediated litter decomposition, which may favour invertebrate‐mediated litter decomposition leading to a depletion of litter stocks in streams. 相似文献
70.
The apocrine and sebaceous scent glands of ringtailed lemurs (Lemur catta) appear to serve different social functions. In behavioral experiments, lemurs modulate their responses to scent marks based on the type of odorant, their own physiological state, the signaler's physiological state, and prior social experience. To examine variation in odorant chemistry relative to olfactory behavior, we used gas chromatography and mass spectrometry to analyze over 86 samples of glandular secretion collected over 2 years from 15 adult lemurs. Labial and scrotal secretions contained organic acids and esters, whereas male brachial secretions were composed almost entirely of squalene and cholesterol derivatives. Principal component and linear discriminant analyses revealed glandular, individual-specific, and seasonal variation in chemical profiles but no relationship to the signaler's social status. The chemical composition of the various secretions provides further clues about the function of the different glands: the higher molecular weight compounds in genital and brachial secretions may increase signal longevity and provide lasting information to conspecifics, consistent with a role in advertising resource ownership or reproductive state. Conversely, the lower molecular weight compounds of antebrachial secretions produce ephemeral signals used primarily in social dominance displays and require integration of multiple sensory modalities for effective signal transmission. 相似文献