全文获取类型
收费全文 | 204篇 |
免费 | 18篇 |
出版年
2022年 | 2篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2015年 | 7篇 |
2013年 | 7篇 |
2012年 | 9篇 |
2011年 | 8篇 |
2010年 | 8篇 |
2009年 | 10篇 |
2008年 | 13篇 |
2007年 | 7篇 |
2006年 | 9篇 |
2005年 | 6篇 |
2004年 | 2篇 |
2003年 | 5篇 |
2002年 | 7篇 |
2001年 | 5篇 |
2000年 | 9篇 |
1999年 | 13篇 |
1998年 | 6篇 |
1997年 | 9篇 |
1996年 | 3篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1977年 | 3篇 |
1975年 | 2篇 |
1969年 | 2篇 |
1960年 | 3篇 |
1959年 | 2篇 |
1957年 | 2篇 |
1954年 | 1篇 |
1951年 | 2篇 |
1949年 | 1篇 |
1947年 | 1篇 |
1946年 | 1篇 |
1943年 | 1篇 |
1942年 | 1篇 |
1941年 | 3篇 |
1940年 | 1篇 |
1939年 | 2篇 |
1938年 | 2篇 |
1937年 | 3篇 |
1932年 | 1篇 |
1921年 | 1篇 |
排序方式: 共有222条查询结果,搜索用时 15 毫秒
161.
Maddalena Salvalaio Nicholas Oliver Deniz Tiknaz Maximillian Schwarze Nicolas Kral Soo-Jeong Kim Giovanni Sena 《Plant physiology》2022,188(3):1604
Efficient foraging by plant roots relies on the ability to sense multiple physical and chemical cues in soil and to reorient growth accordingly (tropism). Root tropisms range from sensing gravity (gravitropism), light (phototropism), water (hydrotropism), touch (thigmotropism), and more. Electrotropism, also known as galvanotropism, is the phenomenon of aligning growth with external electric fields and currents. Although root electrotropism has been observed in a few species since the end of the 19th century, its molecular and physical mechanisms remain elusive, limiting its comparison with the more well-defined sensing pathways in plants. Here, we provide a quantitative and molecular characterization of root electrotropism in the model system Arabidopsis (Arabidopsis thaliana), showing that it does not depend on an asymmetric distribution of the plant hormone auxin, but instead requires the biosynthesis of a second hormone, cytokinin. We also show that the dose–response kinetics of the early steps of root electrotropism follows a power law analogous to the one observed in some physiological reactions in animals. Future studies involving more extensive molecular and quantitative characterization of root electrotropism would represent a step toward a better understanding of signal integration in plants and would also serve as an independent outgroup for comparative analysis of electroreception in animals and fungi. 相似文献
162.
Yuval Itan Bryony L Jones Catherine JE Ingram Dallas M Swallow Mark G Thomas 《BMC evolutionary biology》2010,10(1):36
Background
The ability of adult humans to digest the milk sugar lactose - lactase persistence - is a dominant Mendelian trait that has been a subject of extensive genetic, medical and evolutionary research. Lactase persistence is common in people of European ancestry as well as some African, Middle Eastern and Southern Asian groups, but is rare or absent elsewhere in the world. The recent identification of independent nucleotide changes that are strongly associated with lactase persistence in different populations worldwide has led to the possibility of genetic tests for the trait. However, it is highly unlikely that all lactase persistence-associated variants are known. Using an extensive database of lactase persistence phenotype frequencies, together with information on how those data were collected and data on the frequencies of lactase persistence variants, we present a global summary of the extent to which current genetic knowledge can explain lactase persistence phenotype frequency. 相似文献163.
M. Schubert J. Engel L. Th?ny-Meyer F. W. M. R. Schwarze J. Ihssen 《Applied and environmental microbiology》2012,78(20):7267-7275
In the present work, Norway spruce wood (Picea abies L.) was reacted with a commercial Trametes versicolor laccase in the presence of potassium iodide salt or the phenolic compounds thymol and isoeugenol to impart an antimicrobial property to the wood surface. In order to assess the efficacy of the wood treatment, a leaching of the iodinated and polymerized wood and two biotests including bacteria, a yeast, blue stain fungi, and wood decay fungi were performed. After laccase-catalyzed oxidation of the phenols, the antimicrobial effect was significantly reduced. In contrast, the enzymatic oxidation of iodide (I−) to iodine (I2) in the presence of wood led to an enhanced resistance of the wood surface against all microorganisms, even after exposure to leaching. The efficiency of the enzymatic wood iodination was comparable to that of a chemical wood preservative, VP 7/260a. The modification of the lignocellulose by the laccase-catalyzed iodination was assessed by the Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) technique. The intensities of the selected lignin-associated bands and carbohydrate reference bands were analyzed, and the results indicated a structural change in the lignin matrix. The results suggest that the laccase-catalyzed iodination of the wood surface presents an efficient and ecofriendly method for wood protection. 相似文献
164.
E W Schwarze 《Histochemistry》1975,44(1):103-104
The morphology and the histochemically proofed enzyme pattern of the (Kolmer's) epiplexus cells found at the telencephalic chorioid plexus of humans indicate that these cells are of monocytogenetic origin, that they are monocytes and monocytogenetic macrophages respectively. 相似文献
165.
166.
167.
LJ Melchers MJAM Clausen MF Mastik L Slagter-Menkema JE van der Wal GBA Wisman JLN Roodenburg E Schuuring 《Epigenetics》2015,10(9):850-860
Hypermethylation is an important mechanism for the dynamic regulation of gene expression, necessary for metastasizing tumour cells. Our aim is to identify methylation tumour markers that have a predictive value for the presence of regional lymph node metastases in patients with oral and oropharyngeal squamous cell carcinoma (OOSCC). Significantly differentially expressed genes were retrieved from four reported microarray expression profiles comparing pN0 and pN+ head-neck tumours, and one expression array identifying functionally hypermethylated genes. Additional metastasis-associated genes were included from the literature. Thus genes were selected that influence the development of nodal metastases and might be regulated by methylation. Methylation-specific PCR (MSP) primers were designed and tested on 8 head-neck squamous cell carcinoma cell lines and technically validated on 10 formalin-fixed paraffin-embedded (FFPE) OOSCC cases. Predictive value was assessed in a clinical series of 70 FFPE OOSCC with pathologically determined nodal status. Five out of 28 methylation markers (OCLN, CDKN2A, MGMT,
MLH1 and DAPK1) were frequently differentially methylated in OOSCC. Of these, MGMT methylation was associated with pN0 status (P = 0.02) and with lower immunoexpression (P = 0.02). DAPK1 methylation was associated with pN+ status (P = 0.008) but did not associate with protein expression. In conclusion, out of 28 candidate genes, two (7%) showed a predictive value for the pN status. Both genes, DAPK1 and MGMT, have predictive value for nodal metastasis in a clinical group of OOSCC. Therefore DNA methylation markers are capable of contributing to diagnosis and treatment selection in OOSCC. To efficiently identify additional new methylation markers, genome-wide methods are needed. 相似文献
168.
169.
Blocking the QB‐binding site of photosystem II by tenuazonic acid,a non–host‐specific toxin of Alternaria alternata,activates singlet oxygen‐mediated and EXECUTER‐dependent signalling in Arabidopsis
下载免费PDF全文
![点击此处可从《Plant, cell & environment》网站下载免费的PDF全文](/ch/ext_images/free.gif)
SHIGUO CHEN CHANHONG KIM JE MIN LEE HYUN‐AH LEE ZHANGJUN FEI LIANGSHENG WANG KLAUS APEL 《Plant, cell & environment》2015,38(6):1069-1080
Necrotrophic fungal pathogens produce toxic compounds that induce cell death in infected plants. Often, the primary targets of these toxins and the way a plant responds to them are not known. In the present work, the effect of tenuazonic acid (TeA), a non–host‐specific toxin of Alternaria alternata, on Arabidopsis thaliana has been analysed. TeA blocks the QB‐binding site at the acceptor side of photosystem II (PSII). As a result, charge recombination at the reaction centre (RC) of PSII is expected to enhance the formation of the excited triplet state of the RC chlorophyll that promotes generation of singlet oxygen (1O2). 1O2 activates a signalling pathway that depends on the two EXECUTER (EX) proteins EX1 and EX2 and triggers a programmed cell death response. In seedlings treated with TeA at half‐inhibition concentration 1O2‐mediated and EX‐dependent signalling is activated as indicated by the rapid and transient up‐regulation of 1O2‐responsive genes in wild type, and its suppression in ex1/ex2 mutants. Lesion formation occurs when seedlings are exposed to higher concentrations of TeA for a longer period of time. Under these conditions, the programmed cell death response triggered by 1O2‐mediated and EX‐dependent signalling is superimposed by other events that also contribute to lesion formation. 相似文献
170.
Witte K Schuh AL Hegermann J Sarkeshik A Mayers JR Schwarze K Yates JR Eimer S Audhya A 《Nature cell biology》2011,13(5):550-558
Export of proteins from the endoplasmic reticulum in COPII-coated vesicles occurs at defined sites that contain the scaffolding protein Sec16. We identify TFG-1, a new conserved regulator of protein secretion that interacts directly with SEC-16 and controls the export of cargoes from the endoplasmic reticulum in Caenorhabditis elegans. Hydrodynamic studies indicate that TFG-1 forms hexamers that facilitate the co-assembly of SEC-16 with COPII subunits. Consistent with these findings, TFG-1 depletion leads to a marked decline in both SEC-16 and COPII levels at endoplasmic reticulum exit sites. The sequence encoding the amino terminus of human TFG has been previously identified in chromosome translocation events involving two protein kinases, which created a pair of oncogenes. We propose that fusion of these kinases to TFG relocalizes their activities to endoplasmic reticulum exit sites, where they prematurely phosphorylate substrates during endoplasmic reticulum export. Our findings provide a mechanism by which translocations involving TFG can result in cellular transformation and oncogenesis. 相似文献