Developing Indigenous Rights

Indigenous Peoples, Ethnic Groups and the State. David Maybury-Lewis. Boston, MA: Allyn and Bacon, 1997.168 pp.
Malaysia and the Original People:. Case Study of the Impact of Development on Indigenous Peoples. Robert Knox Dentan. Kirk Endicott. Alberto G. Gomes. and M. B. Hooker. Boston, MA: Allyn and Bacon, 1997. 175 pp.
Forest Dwellers, Forest Protectors: Indigenous Models for International Development. Richard Reed. Boston, MA: Allyn and Bacon, 1997. 135 pp.     

Quantitative comparison of infection of neural cell and fibroblast monolayers by two strains of Toxoplasma gondii

Disease caused by the coccidian Toxoplasma gondii can be confined to the central nervous system, although the parasite is capable of infecting all organ systems. To determine whether neural cells are differentially susceptible to infection and destruction by T. gondii, infection of neonatal mouse brain monolayers was compared to infection of human fibroblast monolayers under the same conditions with equal inocula of two parasite strains. In preliminary experiments there was no difference in total parasite yield or in plaques per monolayer between rodent and human cells. A standardized inoculum of T. gondii RH strain caused 35.6 +/- 6.4 (SD) plaques per well in neural explant monolayers compared to 39.3 +/- 12.5 plaques per well in fibroblasts. T. gondii P strain produced 35.6 +/- 8.9 infected foci per well in neural cells compared to 32.6 +/- 9.3 foci in fibroblasts. Intrinsic properties of neural cells do not appear to cause a higher rate of infection than that in nonneural cells.     

Isolation and partial purification of ceruloplasmin messenger RNA from rat liver

Partially purified ceruloplasmin mRNA was isolated using indirect immunoprecipitation of rat liver polysomes and poly(U)-Sepharose chromatography of polysomal RNA. This RNA programmed the synthesis of ceruloplasmin polypeptides in a cell-free system from mitochondria. Immunochemical analysis of the translation products revealed a 40-fold enrichment of the ceruloplasmin mRNA activity. The purified ceruloplasmin mRNA migrated as a major homogeneous component with an apparent molecular weight about 1×10⁶ daltons in polyacrylamide gels containing sodium dodecyl sulfate. The immunoprecipitated products of the cell-free translation had molecular weights in the range 4.5–5.4×10⁴ daltons as estimated by gel-electrophoresis under denaturating conditions. These values approach the weight of the half-molecule of native ceruloplasmin.     

Toxoplasma gondii: characterization of monoclonal antibodies that recognize rhoptries

We have previously reported on a series of monoclonal antibodies that recognize the rhoptries of Toxoplasma gondii and that interfere with the action of penetration enhancing factor. The antibodies immunoprecipitate several related antigens from [35S]methionine-labeled parasites that range in size from 60 to 43 kDa. By immunoblot, one of the antibodies reacts with the 60 kDa protein in the presence of protease inhibitors. Trypsin digestion of the antigen destroyed antigenic reactivity indicating that the 60 kDa antigen is a protein. The antigen was stable to periodate oxidation and failed to react with Schiff's reagent, indicating that the antigen contains little or no carbohydrate. Two-dimensional gel electrophoresis followed by immunoblot showed that the antigen recognized by Tg 49 was an acidic protein with an approximate pI of 5.8.