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31.
Summary The presence of a coupled Na+/Ca2+ exchange system has been demonstrated in plasma membrane vesicles from rat pancreatic acinar cells. Na+/Ca2+ exchange was investigated by measuring45Ca2+ uptake and45Ca2+ efflux in the presence of sodium gradients and at different electrical potential differences across the membrane (=) in the presence of sodium. Plasma membranes were prepared by a MgCl2 precipitation method and characterized by marker enzyme distribution. When compared to the total homogenate, the typical marker for the plasma membrane, (Na++K+)-ATPase was enriched by 23-fold. Markers for the endoplasmic reticulum, such as RNA and NADPH cytochromec reductase, as well as for mitochondria, the cytochromec oxidase, were reduced by twofold, threefold and 10-fold, respectively. For the Na+/Ca2+ countertransport system, the Ca2+ uptake after 1 min of incubation was half-maximal at 0.62 mol/liter Ca2+ and at 20 mmol/liter Na+ concentration and maximal at 10 mol/liter Ca2+ and 150 mmol/liter Na+ concentration, respecitively. When Na+ was replaced by Li+, maximal Ca2+ uptake was 75% as compared to that in the presence of Na+. Amiloride (10–3 mol/liter) at 200 mmol/liter Na+ did not inhibit Na+/Ca2+ countertransport, whereas at low Na+ concentration (25 mmol/liter) amiloride exhibited dose-dependent inhibition to be 62% at 10–2 mol/liter. CFCCP (10–5 mol/liter) did not influence Na+/Ca2+ countertransport. Monensin inhibited dose dependently; at a concentration of 5×10–6 mol/liter inhibition was 80%. A SCN– or K+ diffusion potential (=), being positive at the vesicle inside, stimulated calcium uptake in the presence of sodium suggesting that Na+/Ca2+ countertransport operates electrogenically, i.e. with a stoichiometry higher than 2 Na+ for 1 Ca2+. In the absence of Na+, did not promote Ca2+ uptake. We conclude that in addition to ATP-dependent Ca2+ outward transport as characterized previously (E. Bayerdörffer, L. Eckhardt, W. Haase & 1. Schulz, 1985,J. Membrane Biol.
84:45–60) the Na+/Ca2+ countertransport system, as characterized in this study, represents a second transport system for the extrusion of calcium from the cell. Furthermore, the high affinity for calcium suggests that this system might participate in the regulation of the cytosolic free Ca2+ level. 相似文献
32.
GEORGE STAFF ERIC N. POWELL ROBERT J. STANTON JR. HAYS CUMMINS 《Lethaia: An International Journal of Palaeontology and Stratigraphy》1985,18(3):209-232
In general, more of the biomass of the community is preserved than is its numerical abundance. Thus, the paleontologist, on the average, works with more of the community when biomass is used. Community characteristics such as taxon dominance and habitat proportions are at least as accurately derived from biomass as numerical abundance. The use of biomass is clearly more appropriate in describing energy flow and trophic proportions. Whenever possible, biomass should be used as a complement to numerical abundance in future paleoecologic reconstructions. 相似文献
33.
The effects of a single post-trial intraperitoneal administration of the dipeptide Litoralon (gamma-L-glutamyl-taurine) and some of its analogues were tested on the passive avoidance latency of male and female Wistar rats. The avoidance latency was significantly decreased by Litoralon and gamma-aminobutyryl-ethanolamine phosphate but lengthened by DL-beta-aminoisobutyryl-ethanolamine phosphate. No differences were observed between the responses of immature male and female rats following Litoralon treatment. The observed inter-group differences in passive avoidance behaviour following dipeptide administration were also demonstrable in tests of the open-field activity of the animals examined immediately after the 24-hour retention test. The results are discussed on the basis of a central Litoralon effect on emotional arousal and the anti-conflict potencies of the dipeptide. 相似文献
34.
Major ecological problems of our polluted troposphere includeairborne toxic chemicals, acid rain and photochemical smog,all three of which are now recognized as being closely relatedchemical phenomena. We also recognize that inorder to developcost-effective strategies for their control, which protect publichealth and the environment, there must be close scientific interactionsbetween chemists and biological scientists. For example, ofrapidly emerging importance is the development of risk assessmentevaluations for specific aspects of each of these problem areas.In preparing such assessments, chemists must define the "exposure,"and biological scientists the "effects." In this paper, I discuss an example of how such close interactionsproved indispensible in our search for atmospheric mutagensand carcinogens. Thus, an integrated chemical/ microbiologicalprocedure for the isolation and identificationof particulatechemical mutagens in respirable diesel soot and ambient particlesis described. Emphasis is placed on our use of the short-term,Ames Salmonella typhimurium bacterial mutagenicity test as arapid, and relatively inexpensive, means of following the biologicalactivities of these environmental mutagens through the chemicalsteps of their separation, isolation and identification fromhighly complex environmental samples. Possible mechanisms offormation of these particulate mutagens are discussed. Theyinclude the reactions of polycyclic aromatic hydrocarbons presenton the surfaces of combustion-generated particles with gaseousco-pollutants such as nitrogen dioxide plus nitric acid, andozone. In discussing this research on a societally "relevant" problem,we illustrate the importance of "Science as a Way of Knowing."We further suggest that this integrated approach to scientificproblem solving by chemical and biological scientists mightserve as an example of a discussion topic on human ecology forundergraduate courses in the natural sciences. 相似文献
35.
Summary Two new microcarriers were tested and showed good properties in cell attachment, cell growth and production of Human--Interferon. Cell densities up to 5·106 cells/ml on microcarriers were reached in 1 l bioreactors. 相似文献
36.
H. Schulz 《Zeitschrift fur angewandte Ichthyologie》1986,2(3):117-124
Turbot (Scophthalmus maximus) of approximate 110 g mean wet weight were fed three different diets supplemented with DL-alpha-tocopherol (1.4 g and 2.45 g · kg-1 basal diet) and with non-alphatocopherols (0.29 g beta-, 1.29 g gamma-and 0.68 g delta-tocopherol kg-1 basal diet). High dosages of alpha-tocopherol caused a linear increase of liver tocopherol (ninefold to controls). Spleen and blood serum accumulated also tocopherol. The level in muscle tissue was only poorly influenced by high vitamin E dosage. Non-alpha-tocopherols which normally do nct occur in blood and tissue have been resorbed from experiment diets and deposed in tissues in the same manner as alpha-tocopherol. The distribution of various tocopherols in the diets was reflected in muscle, spleen and serum of the fishes in the experiment. In the liver the distribution pattern of tocopherols was different to that of the diet. Conversion of alpha-tocopherol to non-alpha-tocopherpls in-vivo has not been found. 相似文献
37.
38.
M. C. GACULA JR. R. W. WASHAM II S. A. BEARD J. E. HEINZE 《Journal of sensory studies》1986,1(1):47-53
In-house use consumer test data from four studies dealing with three pairs of household products and a pair of antiperspirant products were examined for significant carry-over (product usage order) effects, which would confound the analysis of treatment (product) effects. In each study, two products were compared using a two-period crossover design. One hundred twenty panelists participated in each study. A forced choice preference scale or a 9-point hedonic scale was used to obtain responses from various sensory attributes. In all studies, the estimates of carry-over effects were not significant at the 5% level. Transformation of hedonic scale data into preference dichotomy also gave estimates of carry-over effects which were not significant at the 5% level, but led to a loss of test sensitivity for detecting treatment differences. The authors recommend that all comparative crossover design studies in sensory evaluation be monitored for carry-over effects and that statistically determined sample size should be used to reduce the possibility of obtaining significant carry-over effects. 相似文献
39.
Enhancement of spontaneous and lymphokine activated human macrophage cytotoxicity by hyperthermia 总被引:1,自引:0,他引:1
Human macrophages grown on hydrophobic teflon membranes from blood-born monocytes were incubated at hyperthermic temperatures for various time periods and then tested for their ability to inhibit the growth of an allogeneic lymphoma cell line (U 937). Incubation at 40.5 degrees C greatly enhanced macrophage cytotoxicity. This effect of hyperthermia developed slowly with an optimal incubation period of 48 h. In addition, lymphokine activation of macrophages for cytotoxicity appeared to be more effective at elevated temperatures. 相似文献
40.
The Scientific Board of the California Medical Association presents the following inventory of items of progress in nuclear medicine. Each item, in the judgment of a panel of knowledgeable physicians, has recently become reasonably firmly established, both as to scientific fact and important clinical significance. The items are presented in simple epitome and an authoritative reference, both to the item itself and to the subject as a whole, is generally given for those who may be unfamiliar with a particular item. The purpose is to assist busy practitioners, students, research workers or scholars to stay abreast of these items of progress in nuclear medicine that have recently achieved a substantial degree of authoritative acceptance, whether in their own field of special interest or another.The items of progress listed below were selected by the Advisory Panel to the Section on Nuclear Medicine of the California Medical Association and the summaries were prepared under its direction. 相似文献