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41.
Kissel JT Scott CB Reyna SP Crawford TO Simard LR Krosschell KJ Acsadi G Elsheik B Schroth MK D'Anjou G LaSalle B Prior TW Sorenson S Maczulski JA Bromberg MB Chan GM Swoboda KJ;Project Cure Spinal Muscular Atrophy Investigators' Network 《PloS one》2011,6(7):e21296
Background
Multiple lines of evidence have suggested that valproic acid (VPA) might benefit patients with spinal muscular atrophy (SMA). The SMA CARNIVAL TRIAL was a two part prospective trial to evaluate oral VPA and l-carnitine in SMA children. Part 1 targeted non-ambulatory children ages 2–8 in a 12 month cross over design. We report here Part 2, a twelve month prospective, open-label trial of VPA and L-carnitine in ambulatory SMA children.Methods
This study involved 33 genetically proven type 3 SMA subjects ages 3–17 years. Subjects underwent two baseline assessments over 4–6 weeks and then were placed on VPA and L-carnitine for 12 months. Assessments were performed at baseline, 3, 6 and 12 months. Primary outcomes included safety, adverse events and the change at 6 and 12 months in motor function assessed using the Modified Hammersmith Functional Motor Scale Extend (MHFMS-Extend), timed motor tests and fine motor modules. Secondary outcomes included changes in ulnar compound muscle action potential amplitudes (CMAP), handheld dynamometry, pulmonary function, and Pediatric Quality of Life Inventory scores.Results
Twenty-eight subjects completed the study. VPA and carnitine were generally well tolerated. Although adverse events occurred in 85% of subjects, they were usually mild and transient. Weight gain of 20% above body weight occurred in 17% of subjects. There was no significant change in any primary outcome at six or 12 months. Some pulmonary function measures showed improvement at one year as expected with normal growth. CMAP significantly improved suggesting a modest biologic effect not clinically meaningful.Conclusions
This study, coupled with the CARNIVAL Part 1 study, indicate that VPA is not effective in improving strength or function in SMA children. The outcomes used in this study are feasible and reliable, and can be employed in future trials in SMA.Trial Regsitration
Clinicaltrials.gov NCT00227266相似文献42.
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44.
The intrinsic structure and stability of out-of-alternation base pairs in Z-DNA. 总被引:1,自引:1,他引:0 下载免费PDF全文
Alternating pyrimidine-purine sequences typically form Z-DNA, with the pyrimidines in the anti and purines in the syn conformations. The observation that dC and dT nucleotides can also adopt the syn conformation (i.e. the nucleotides are out-of-alternation) extends the range of sequences that can convert to this left-handed form of DNA. Here, we study the effects of placing two adjacent d(G*C) base pairs as opposed to a single d(G*C) base pair or two d(A*T) base pairs out-of-alternation by comparing the structure of d(m5CGGCm5CG)2with the previously published structures of d(m5CGGGm5CG)*d(m5CGCCm5CG) and d(m5CGATm5CG)2. A high buckle and loss of stacking interactions are observed as intrinsic properties of the out-of-alternation base pairs regardless of sequence and the context of the dinucleotide. From solution titrations, we find that the destabilizing effect of out-of-alternation d(G*C) base pairs are identical whether these base pairs are adjacent or isolated. We can therefore conclude that it is these intrinsic distortions in the structure of the base pairs and not neighboring effects that account for the inability of out-of-alternation base pairs to adopt the left-handed Z conformation. 相似文献
45.
Meike G?lzenleuchter Rahul Kanwar Manal Zaibak Fadi Al Saiegh Theresa Hartung Jana Klukas Regenia L Smalley Julie M Cunningham Maria E Figueroa Gary P Schroth Terry M Therneau Michaela S Banck Andreas S Beutler 《Epigenetics》2015,10(3):200-212
The response of the peripheral nervous system (PNS) to injury may go together with alterations in epigenetics, a conjecture that has not been subjected to a comprehensive, genome-wide test. Using reduced representation bisulfite sequencing, we report widespread remodeling of DNA methylation in the rat dorsal root ganglion (DRG) occurring within 24 h of peripheral nerve ligation, a neuropathy model of allodynia. Significant (P < 10−4) cytosine hyper- and hypo-methylation was found at thousands of CpG sites. Remodeling occurred outside of CpG islands. Changes affected genes with known roles in the PNS, yet methylome remodeling also involved genes that were not linked to neuroplasticity by prior evidence. Consistent with emerging models relying on genome-wide methylation and RNA-seq analysis of promoter regions and gene bodies, variation of methylation was not tightly linked with variation of gene expression. Furthermore, approximately 44% of the dynamically changed CpGs were located outside of genes. We compared their positions with the intergenic, tissue-specific differentially methylated CpGs (tDMCs) of an independent experimental set consisting of liver, spleen, L4 control DRG, and muscle. Dynamic changes affected those intergenic CpGs that were different between tissues (P < 10−15) and almost never the invariant portion of the methylome (those CpGs that were identical across all tissues). Our findings—obtained in mixed tissue—show that peripheral nerve injury leads to methylome remodeling in the DRG. Future studies may address which of the cell types found in the DRG, such as specific groups of neurons or non-neuronal cells are affected by which aspect of the observed methylome remodeling. 相似文献
46.
Naiara Akizu Jennifer L. Silhavy Rasim Ozgur Rosti Eric Scott Ali G. Fenstermaker Jana Schroth Maha S. Zaki Henry Sanchez Neerja Gupta Madhulika Kabra Majdi Kara Tawfeg Ben-Omran Basak Rosti Alicia Guemez-Gamboa Emily Spencer Roger Pan Na Cai Mostafa Abdellateef Stacey Gabriel Jan Halbritter Friedhelm Hildebrandt Hans van Bokhoven Murat Gunel Joseph G. Gleeson 《American journal of human genetics》2014
47.
48.
Helmut Bürgmann Jutta Kleikemper Laurence Duc Michael Bunge Martin H. Schroth Josef Zeyer 《Bioremediation Journal》2008,12(4):193-209
Detection and quantification of bacteria related to Dehalococcoides is essential for the development of effective remediation strategies for tetrachloroethene (PCE)-contaminated sites. In this study, the authors applied three methods for quantifying Dehalococcoides-like bacteria in a PCE-contaminated aquifer undergoing natural attenuation in Grenchen, Switzerland: a catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) protocol, a competitive nested polymerase chain reaction (PCR) approach, and a direct PCR end point quantification with external standards. For the investigated aquifer, multiple lines of evidence indicated that reductive dechlorination (and likely dehalorespiration) was an active process. Both PCR-based quantification methods indicated that low numbers of mostly sediment-bound Dehalococcoides were present in the contaminated zone of the Grenchen aquifer. Estimates based on the quantitative PCR methods ranged from 2.1 × 107 to 1.5 × 108 sediment-bound Dehalococcoides 16S rRNA gene copies per liter of aquifer volume. In contrast, the liquid phase only contained between 8 and 80 copies per liter aquifer volume. CARD-FISH was not sensitive enough for the quantification of Dehalococcoides cell numbers in this aquifer. Cloning and sequencing of the PCR products revealed the presence of sequences closely related to Dehalococcoides isolates such as D. ethenogenes and Dehalococcoides sp. BAV1. An apparently abundant group (termed “Grenchen Cluster”) of sequences more distantly related to Dehalococcoides was also identified, so far without cultured representatives. 相似文献
49.
Batista PJ Ruby JG Claycomb JM Chiang R Fahlgren N Kasschau KD Chaves DA Gu W Vasale JJ Duan S Conte D Luo S Schroth GP Carrington JC Bartel DP Mello CC 《Molecular cell》2008,31(1):67-78
In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-RNA loci share a conserved upstream sequence motif, the mature 21U-RNAs are not conserved and, with few exceptions, fail to exhibit complementarity or evidence for direct regulation of other expressed sequences. Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility. 相似文献
50.
A nutritional screen of 143 carbon sources was done on 88 strains of xanthomonads from 39 different Xanthomonas campestris pathovars, X. albilineans, X.fragariae , and ' X. gardneri '. Six compounds, cellobiose, fructose, fumarate, glucose, L-malate and succinate supported growth of all strains except X. albilineans , whereas 92 substrates were not utilized by any strain. Substrate utilization patterns appeared sufficiently uniform among the various genomic groups within Xanthomonas to allow their differentiation. The most easily distinguished pathovars were X. cam . pv. oryzicola and X. cam. secalis of genomic groups 4 and 3, respectively, because they used few substrates. Genomic group 1 was the most difficult to distinguish because utilization patterns differed substantially among the pathovars that comprise the group. Substrate utilization was useful for distinguishing pathovars within genomic groups. For example, X. campestris pv. pelargonii of genomic group 5 was differentiated from X. cam. carotae, X. cam. taraxaci , and ' X. gardneri ' by growth on aconitate but not D-tartrate. Similarly, use of D-tartrate differentiated X. celebensis from X. cam. pv. juglandis within group 6. Sorbitol was utilized only by X. cam. pv. plantaginis of group 2 and arabitol was a useful substrate for identifying X. cam. pv. pisi and pv. eucalypti . Most patterns of carbon utilization were confirmed with Biolog tests but there were exceptions as was found with utilization of glycerol and D-arabitol. The Biolog test also revealed some differences in carbon utilization not detected by standard tests of carbon substrates. It is concluded that nutritional screening has promise for identifying genomic groups and various pathovars within the genus Xanthomonas . 相似文献