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31.
T-DNA tagging in the model legume Medicago truncatula allows efficient gene discovery 总被引:3,自引:0,他引:3
Scholte Marije d'Erfurth Isabelle Rippa Sonia Mondy Samuel Cosson Viviane Durand Patricia Breda Colette Trinh Hanh Rodriguez-Llorente Ignacio Kondorosi Eva Schultze Michael Kondorosi Adam Ratet Pascal 《Molecular breeding : new strategies in plant improvement》2002,10(4):203-215
The annual legume Medicago truncatula has been proposed as a model plant to study various aspects of legume biology including rhizobial and mycorrhizal symbiosis because it is well suited for the genetic analysis of these processes . To facilitate the characterization of M. truncatula genes participating in various developmental processes we have initiated an insertion mutagenesis program in this plant using three different T-DNAs as tags. To investigate which type of vector is the most suitable for mutagenesis we compared the behavior of these T-DNAs. One T-DNA vector was a derivative of pBin19 and plant selection was based on kanamycin resistance. The two other vectors carried T-DNA conferring Basta resistance in the transgenic plants. For each T-DNA type, we determined the copy number in the transgenic lines, the structure of the T-DNA loci and the sequences of the integration sites. The T-DNA derived from pBin19 generated complex T-DNA insertion patterns. The two others generally gave single copy T-DNA inserts that could result in gene fusions for the pGKB5 T-DNA. Analysis of the T-DNA borders revealed that several M. truncatula genes were tagged in these transgenic lines and in vivo gus fusions were also obtained. These results demonstrate that T-DNA tagging can efficiently be used in M. truncatula for gene discovery. 相似文献
32.
Cavelier Florine Enjalbal Christine Santolini Jérome Haraux Francis Sigalat Claude Verducci Jean André François 《International journal of peptide research and therapeutics》1997,4(4-6):283-288
Summary Tentoxin [cyclo-(MeAla1-Leu2-MeΔPhe3-Gly4] is a metabolite isolated from a phytopathogenic fungusAlternaria alternata, which induces chlorosis of many plants. This potential natural herbicide binds specifically to the soluble part CF1 of the chloroplastic coupling factor, which is a proton ATP-synthase. The effect of the toxin is concentration dependent:
at low concentration it is a powerful inhibitor, while at higher concentration, it stimulates the enzyme. We synthesized tentoxin
and we designed new analogues in order to vary the hydrophobicity on the side chain and to study the structure activity relationships.
Comparative activities suggest that it is possible to separate inhibitory and activating effects using tentoxin analogues,
showing some evidence for the existence of two binding sites with different affinity constant. 相似文献
33.
Nicolas Loiseau Florine Cavelier Jean-Pierre Noel Jean-Marie Gomis 《Journal of peptide science》2002,8(7):335-346
Tentoxin is a naturally occurring phytotoxic cyclic tetrapeptide excreted by fungi of the Alternaria alternata family. The four total syntheses of tentoxin published to date give poor total yields, mainly owing to two difficulties, the introduction of the dehydro amino acid and more especially the cyclization step. Here we describe a method that stereospecifically introduces Z-dehydrophenylalanine (deltaZPhe) by a modified Erlenmeyer aldolization reaction. The linear tetrapeptide, Boc-R1Ala-Leu-R2deltaZPhe-G1y-OMe (R1, R2: CH3, 14CH3), the precursor of tentoxin, was obtained in a 72% yield from Boc-Leu-Gly-OH. This linear tetrapeptide, labelled with carbon-14, was used for a comparative study of four cyclization reagents DPPA, DCC-PfpOH, HBTU and HATU. This last was the most effective and gave tentoxin in a 81% cyclization yield. The activated ester formed with this reagent displayed an enhanced capacity for cyclization, permitting cyclization in concentrated medium (10 mM). This new synthetic route gave tentoxin in a 60% yield from Boc-Leu-Gly-OH and offers a means of achieving the synthesis of hitherto elusive analogues. 相似文献
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D. van Erck C. D. Dolman M. Snaterse M. Tieland A. H. G. Driessen P. J. M. Weijs W. J. M. Scholte op Reimer J. P. Henriques J. D. Schoufour 《Netherlands heart journal》2021,29(9):460
BackgroundThe COVID-19 pandemic has led to a national lockdown in the Netherlands, which also affected transcatheter aortic valve implantation (TAVI) patients. The objective of the study was to describe physical activity, dietary intake and quality of life (QoL) in patients on the waiting list for TAVI pre-lockdown and during lockdown.MethodsConsecutive patients awaiting TAVI at the Amsterdam University Medical Centers, the Netherlands were included. Measurements were self-reported effect of lockdown, physical activity, dietary intake and QoL.ResultsIn total, 58 patients (median age 80, interquartile range (IQR) 76–84, 45% female) were observed pre-lockdown and 16 patients (median age 78, IQR 76–82, 25% female) during lockdown. Ten of the 16 patients during lockdown reported a decline in physical activity. However, we observed a median number of 5861 steps a day (IQR 4579–7074) pre-lockdown and 8404 steps a day (IQR 7653–10,829) during lockdown. Median daily protein intake was 69 g (IQR 59–82) pre-lockdown and 90 g (IQR 68–107) during lockdown. Self-rated health on a visual analogue scale was 63 points (IQR 51–74) pre-lockdown and 73 points (IQR 65–86) during lockdown.ConclusionsMore than half of the patients during lockdown reported less physical activity, while we observed a higher number of steps a day, a similar dietary intake and a higher QoL. Therefore, patients on the TAVI waiting list appeared to be able to cope with the lockdown measures. 相似文献
38.
Georgios A. Dalkas Damien Marchand Jean‐Claude Galleyrand Jean Martinez Georgios A. Spyroulias Paul Cordopatis Florine Cavelier 《Journal of peptide science》2010,16(2):91-97
Human ACE is a central component of the renin–angiotensin system and a major therapeutic target for cardiovascular diseases. The somatic form of the enzyme (sACE) comprises two homologous metallopeptidase domains (N and C), each bearing a zinc active site with similar but distinct substrate and inhibitor specificities. In this study, we present the biological activity of silacaptopril, a silylated analogue of captopril, and its binding affinity towards ACE. Based on the recently determined crystal structures of both the ACE domains, a series of docking calculations were carried out in order to study the structural characteristics and the binding properties of silacaptopril and its analogues with ACE. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
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N. T. B. Scholte M. J. Lenzen B. van der Hoven W. J. R. Rietdijk H. J. Metselaar C. A. den Uil 《Netherlands heart journal》2018,26(10):506-511