全文获取类型
收费全文 | 3039篇 |
免费 | 331篇 |
国内免费 | 4篇 |
出版年
2021年 | 24篇 |
2020年 | 26篇 |
2019年 | 27篇 |
2018年 | 30篇 |
2017年 | 36篇 |
2016年 | 46篇 |
2015年 | 96篇 |
2014年 | 80篇 |
2013年 | 104篇 |
2012年 | 130篇 |
2011年 | 110篇 |
2010年 | 92篇 |
2009年 | 61篇 |
2008年 | 118篇 |
2007年 | 126篇 |
2006年 | 109篇 |
2005年 | 107篇 |
2004年 | 92篇 |
2003年 | 99篇 |
2002年 | 127篇 |
2001年 | 107篇 |
2000年 | 123篇 |
1999年 | 113篇 |
1998年 | 68篇 |
1997年 | 36篇 |
1996年 | 50篇 |
1995年 | 32篇 |
1994年 | 33篇 |
1993年 | 47篇 |
1992年 | 76篇 |
1991年 | 78篇 |
1990年 | 76篇 |
1989年 | 57篇 |
1988年 | 62篇 |
1987年 | 58篇 |
1986年 | 55篇 |
1985年 | 40篇 |
1984年 | 53篇 |
1983年 | 40篇 |
1982年 | 41篇 |
1981年 | 35篇 |
1979年 | 38篇 |
1978年 | 37篇 |
1976年 | 32篇 |
1975年 | 47篇 |
1974年 | 29篇 |
1973年 | 26篇 |
1972年 | 36篇 |
1971年 | 25篇 |
1967年 | 23篇 |
排序方式: 共有3374条查询结果,搜索用时 31 毫秒
121.
Summary A partial trisomy for the distal segment of the long arm of chromosome 7 (bands q32qter) was observed in a severely retarded child with somatic and CNS anomalies. The phenotypically normal father and paternal grandmother had a balanced reciprocal translocation between the long arm of a chromosome 2 and the long arm of a chromosome 7: 46,XX-XY,t(2;7) (q37;q32). The clinical features of the child at birth and at the ages of 5 months and 2 years are compared with those previously reported in cases of partial trisomy 7q. 相似文献
122.
123.
124.
The reversibility of the binding of ionophores to the thylakoid membrane is studied. While gramicidin binds practically irreversibly, valinomycin and nonactin bind reversibly, however, only a small fraction (about 1 %) of the membrane-bound valinomycin or nonactin is active in ion transport. The current-voltage relationship is evaluated under these circumstances. We have found that it is practically linear. This together with the relationship between current and ion concentration agrees qualitatively with the results reported for bimolecular lipid membranes, which contain a large fraction of negatively charged lipids. For the ionophores, valinomycin and nonactin, the binding equilibria (K ≈ 104) and the turnover numbers (≈ 3 · 104/s) are evaluated for their action on the thylakoid membrane. Possible reasons for the inactivity of the majority of membrane-bound ionophore molecules are discussed. 相似文献
125.
126.
The use of several energy-coupling reactions in characterizing mutants of Escherichia coli K12 defective in oxidative phosphorylation. 总被引:8,自引:0,他引:8
Oxidative phosphorylation, ATP-32Pi exchange, ATP-dependent quenching of acridine-dye fluorescence, ATP-dependent transhydrogenase and ATP-dependent transport of thiomethyl beta-D-galactoside are shown to be experimentally equivalent tools to study the functional state of the ATPase complex in Escherichia coli wild-type and mutant strains defective in oxidative phosphorylation. According to these criteria ten mutants in the ATPase complex were classified having lesions in the unc A,B region of the chromosome. The first mutant type lacks ATPase activity, but the membrane-integrated part of the complex remains functional (class I). The second mutant type lacks a functional membrane-integrated part, but retains ATPase activity (class II). The third mutant type is shown to be defective in both parts of the ATPase complex (class III). 相似文献
127.
In the second part of this series, previous solvent limitations in infrared studies are discussed and novel polar solvent systems for the analysis of nonpolar and polar lipids described. Limitation of potassium bromide windows for infrared cells are discussed. The use of calcium fluoride cells with crude lipids is discussed. Problems related to hydrogen bonding of lipid solutes in various solvent systems are discussed as well as hydrogen-deuterium exchange in biologically important lipids. 相似文献
128.
The Isolation and Characterization of Adenosine Monophosphate-rich Polynucleotides Synthesized by Soybean Hypocotyl Cells: Their Relation to Messenger Ribonucleic Acid
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Plant ribonucleic acids which have high adenosine monophosphate concentrations were studied. Purified deoxyribonucleic acid-like ribonucleic acid and tenaciously bound ribonucleic acid fractions both contained poly-adenosine monophosphate sequences (those from the latter being longer than those from the former); without these poly-adenosine monophosphate sequences their base compositions were the same. The average poly-adenosine monophosphate sequence from purified tenaciously bound ribonucleic acid was 160 residues long, as measured by gel electrophoresis. However, base hydrolysis and chromatography indicated one 3′-nucleoside (adenosine) per 71 nucleotides, giving a chain length of 72 residues. The dominant species in the cytoplasm, as measured by radioactive precursor incorporation, was tenaciously bound ribonucleic acid, whereas deoxyribonucleic acid-like ribonucleic acid was present in greater amounts in the nucleus. This work provides evidence that deoxyribonucleic acid-like ribonucleic acid and tenaciously bound ribonucleic acid represent forms of messenger ribonucleic acid in soybean, with deoxyribonucleic acid-like ribonucleic acid residing in the nucleus, perhaps as the messenger ribonucleic acid precursor, and tenaciously bound ribonucleic acid residing, as the active messenger ribonucleic acid, in the cytoplasm. 相似文献
129.
Mapping QTL for production traits in segregating Piétrain pig populations using genome‐wide association study results of F2 crosses
下载免费PDF全文
![点击此处可从《Animal genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
In this study, genome‐wide association study (GWAS) results of porcine F2 crosses were used to map QTL in outcross Piétrain populations. For this purpose, two F2 crosses (Piétrain × Meishan, n = 304; Piétrain × Wild Boar, n = 291) were genotyped with the PorcineSNP60v2 BeadChip and phenotyped for the dressing yield, carcass length, daily gain and drip loss traits. GWASs were conducted in the pooled F2 cross applying single marker mixed linear models. For the investigated traits, between two and five (in total 15) QTL core regions, spanning 250 segregating SNPs around a significant trait‐associated peak SNP, were identified. The SNPs within the QTL core regions were subsequently tested for trait association in two outcross Piétrain populations consisting of 771 progeny‐tested boars and 210 sows with their own performance records. In the sow (boar) dataset, five (eight) of the 15 mapped QTL were validated. Hence, many QTL mapped in the F2 crosses (with Piétrain as a common founder breed) are still segregating in the current Piétrain breed. This confirms the usefulness of existing F2 crosses for mapping QTL that are still segregating in the recent founder breed generation. The approach utilizes the high power of an F2 cross to map QTL in a breeding population for which it is not guaranteed that they would be found using a GWAS in this population. 相似文献
130.