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排序方式: 共有108条查询结果,搜索用时 15 毫秒
81.
82.
Prevalence and parental origin of de novo RET mutations in multiple endocrine neoplasia type 2A and familial medullary thyroid carcinoma. Le Groupe d'Etude des Tumeurs a Calcitonine. 下载免费PDF全文
83.
Schlumberger MC Käppeli R Wetter M Müller AJ Misselwitz B Dilling S Kremer M Hardt WD 《Molecular microbiology》2007,65(3):741-760
Salmonella Typhimurium causes bacterial enterocolitis. The type III secretion system (TTSS)-1 is a key virulence determinant of S. Typhimurium mediating host cell invasion and acute enterocolitis. The TTSS-1 effector protein SipA is transported into host cells, accumulates in characteristic foci at the bacteria-host cell interface, manipulates signalling and affects virulence. Two functional domains of SipA have previously been characterized: The N-terminal SipA region (amino acids 1-105) mediates TTSS-1 transport and the C-terminal SipA 'actin-binding' domain (ABD; amino acids 446-685) manipulates F-actin assembly. Little is known about the central region of SipA. In a deletion analysis we found that the central SipA region harbours two distinct functional domains, F1 and F2. They are involved in SipA focus formation and host manipulation. The F1 domain (amino acids 170-271) drives SipA focus formation and domain F2 (amino acids 280-394) enhances this process by mediating SipA-SipA interactions. SipA variants lacking the F1-, the F2- or the actin binding domain were attenuated in virulence assays, namely host cell invasion and/or virulence in a mouse model for enterocolitis. Our results show that the newly identified SipA domains have distinct functions. Nevertheless, cooperation between the SipA domains F1, F2 and ABD is required to promote Salmonella virulence. 相似文献
84.
H G Opitz U Opitz H Lemke H D Flad G Hewlett H D Schlumberger 《Journal of immunology (Baltimore, Md. : 1950)》1977,119(6):2089-2094
The primary immune response in mouse spleen cell cultures against heterologous red cell antigens is dependent on the medium being supplemented with selected batches of fetal calf serum. Mouse serum itself is not able to support this response. The active immune response-supporting component in fetal calf serum seems to be a distinct factor (s), which has been partially purified by Sephadex G-100 filtration and termed MaSF-2-mercaptoethanol-activated serum factor. In this report it is demonstrated that MaSF is also present in mouse serum. For functional detection, mouse MaSF has to be separated from higher m.w. inhibitors, and has to be activated by 2-ME. After separation and activation mouse MaSF can support the primary immune response in a completely homologous in vitro culture system. Evidence is presented that MaSF can also be activated by macrophages. It is concluded that macrophages and 2-ME have the same mode of action in the primary immune response in vitro, i.e., induction of lymphocyte competence by activation of a serum factor. 相似文献
85.
86.
Valérie Bernard Justine Bouilly Piet Kramer Nadège Carré Martin Schlumberger Jenny A. Visser Jacques Young Nadine Binart 《PloS one》2016,11(4)
The aim of the study was to evaluate ovarian toxicity of tyrosine kinase inhibitor (TKI) sunitinib, since only scarce data are available on gonadal function after this treatment. Six-week-old female mice received orally, once daily, vehicle or sunitinib (50 mg/kg/d) during 5 weeks. Fertility parameters were analyzed from ovulation to litter assessment. Sunitinib exposure significantly reduced (i) corpora lutea number per ovary (1.1 ± 0.38 in sunitinib group versus 4 ± 0.79 in control group, p<0.01) and (ii) serum Anti Müllerian hormone (AMH) levels in sunitinib treated mice (12.01 ± 1.16) compared to control mice (14.33 ± 0.87 ng/ml, p< 0.05). However, primordial and growing follicles numbers per ovary were not different in both groups. After treatment withdrawal, female mice in both groups were able to obtain litters. These data could be helpful to counsel clinicians and patients, when fertility preservation methods are discussed, before TKI treatment in girls and young women. 相似文献
87.
H. L. M. Lelieveld H. Bachmayer B. Boon A. Bennett G. Brunius K. Bürki M. Cantley J.M. Collard C.H. Collins P.Crooy O. Doblhoff-Dier R. Dubakiene I. Economidis A. Elmqvist C. Frontali-Botti R. Havenaar H. Haymerle O. Käppeli G. Leaver M. Lex S. Lund J. L. Mahler R. Marris L. J. Martinez C. Mosgaard M. Nicu C. Normand-Plessier J. Olsen J. Pazlerova M. Romantschuk F. Rudan M. Sarvas H.-D. Schlumberger G. Szvoboda H. Stepankova G. Tzotzos V. Vaicuivenas K. Wagner R. G. Werner A. Zhilevicha 《Applied microbiology and biotechnology》1997,48(2):135-140
The transport of infectious and biological material is regulated by a number of international organizations. This mini-review
has been compiled to increase awareness within the scientific community of problems caused by differences in terminology (such
as infectious materials/substances, biological products, diagnostic specimens, genetically modified microorganisms) and certain
technical aspects of the main international guidelines, and to assist policy makers in the creation of harmonized guidelines.
A list of relevant Internet resources has been compiled.
Received: 3 March 1997 / Accepted: 8 March 1997 相似文献
88.
The effects of selective ibotenate lesions of the complete hippocampus (CHip), the hippocampal ventral pole (VP), or the medial prefrontal cortex (mPFC) in male rats were assessed on several measures related to energy regulation (i.e., body weight gain, food intake, body adiposity, metabolic activity, general behavioral activity, conditioned appetitive responding). The testing conditions were designed to minimize the nonspecific debilitating effects of these surgeries on intake and body weight. Rats with CHip and VP lesions exhibited significantly greater weight gain and food intake compared with controls. Furthermore, CHip-lesioned rats, but not rats with VP lesions, showed elevated metabolic activity, general activity in the dark phase of the light-dark cycle, and greater conditioned appetitive behavior, compared with control rats without these brain lesions. In contrast, rats with mPFC lesions were not different from controls on any of these measures. These results indicate that hippocampal damage interferes with energy and body weight regulation, perhaps by disrupting higher-order learning and memory processes that contribute to the control of appetitive and consummatory behavior. 相似文献
89.
Sébastien Schlumberger Katarina Črnigoj Kristan Katja Ota Robert Frangež Jordi Molgό Kristina Sepčić Evelyne Benoit Peter Maček 《FEBS letters》2014
Proteins from the oyster mushroom, 15 kDa ostreolysin A (OlyA), and 59 kDa pleurotolysin B (PlyB) with a membrane attack complex/perforin (MACPF) domain, damage cell membranes as a binary cytolytic pore-forming complex. Measurements of single-channel conductance and transmembrane macroscopic current reveal that OlyA/PlyB form non-selective ion-conducting pores with broad, skewed conductance distributions in N18 neuroblastoma and CHO-K1 cell membranes. Polyethylene-glycol 8000 (hydrodynamic radius of 3.78 nm) provides almost complete osmotic protection against haemolysis, which strongly suggests a colloid-osmotic type of erythrocyte lysis. Our data indicate that OlyA/PlyB form transmembrane pores of varied sizes, as other pore-forming proteins with a MACPF domain. 相似文献
90.
Benjamin Misselwitz Gerhard Strittmatter Balamurugan Periaswamy Markus C Schlumberger Samuel Rout Peter Horvath Karol Kozak Wolf-Dietrich Hardt 《BMC bioinformatics》2010,11(1):30