全文获取类型
收费全文 | 345篇 |
免费 | 23篇 |
出版年
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 12篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 12篇 |
2017年 | 7篇 |
2016年 | 16篇 |
2015年 | 22篇 |
2014年 | 27篇 |
2013年 | 25篇 |
2012年 | 39篇 |
2011年 | 22篇 |
2010年 | 19篇 |
2009年 | 16篇 |
2008年 | 24篇 |
2007年 | 23篇 |
2006年 | 20篇 |
2005年 | 12篇 |
2004年 | 15篇 |
2003年 | 12篇 |
2002年 | 9篇 |
2001年 | 2篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1985年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有368条查询结果,搜索用时 15 毫秒
361.
Fábián Eszter Horváth Gabriella Opper Balázs Atlasz Tamás Tóth Gábor Reglődi Dóra 《International journal of peptide research and therapeutics》2021,27(2):1221-1228
International Journal of Peptide Research and Therapeutics - The integrity of the innermost, pigment epithelial layer of the retina is crucial for the photoreceptor survival and for maintaining the... 相似文献
362.
Boda Pál Bozóki Tamás Krasznai-K. Eszter Á. Várbíró Gábor Móra Arnold Csabai Zoltán 《Hydrobiologia》2021,848(4):857-871
Hydrobiologia - Stream macroinvertebrates, as non-target organisms, may face with either a single or a complex stressor during a restoration treatment. We quantified the structural, phylogenetic,... 相似文献
363.
The chaperonin GroEL contains two seven-subunit rings, and allosteric signals between them are required to complete the GroEL reaction cycle. For this reason SR1, a mutant of GroEL that forms only single rings, cannot function as a chaperone. Mutations in SR1 that restore chaperone function weaken its interaction with the cochaperonin GroES. We predicted that GroES mutants with reduced affinity for GroEL would also restore function to SR1. To test this, we mutated residues in GroES in and near its contact site with GroEL. Nearly half of the mutants showed partial function with SR1. Two mutants were confirmed to have reduced affinity for GroEL. Intriguingly, some GroES mutants were able to function with active single ring mutants of GroEL. 相似文献
364.
Shensi Shen Guillermo Rodrigo Satya Prakash Eszter Majer Thomas E. Landrain Boris Kirov José-Antonio Daròs Alfonso Jaramillo 《Nucleic acids research》2015,43(10):5158-5170
Organisms have different circuitries that allow converting signal molecule levels to changes in gene expression. An important challenge in synthetic biology involves the de novo design of RNA modules enabling dynamic signal processing in live cells. This requires a scalable methodology for sensing, transmission, and actuation, which could be assembled into larger signaling networks. Here, we present a biochemical strategy to design RNA-mediated signal transduction cascades able to sense small molecules and small RNAs. We design switchable functional RNA domains by using strand-displacement techniques. We experimentally characterize the molecular mechanism underlying our synthetic RNA signaling cascades, show the ability to regulate gene expression with transduced RNA signals, and describe the signal processing response of our systems to periodic forcing in single live cells. The engineered systems integrate RNA–RNA interaction with available ribozyme and aptamer elements, providing new ways to engineer arbitrary complex gene circuits. 相似文献
365.
Andrs Tlas Krisztina Huszr Pter Istvn Kulcsr Julia K Varga va Varga Eszter Tth Zsombor Welker Gergely Erds Pter Ferenc Pach gnes Welker Zoltn Gyrgypl Gbor E Tusndy Ervin Welker 《Nucleic acids research》2021,49(6):e31
Detailed target-selectivity information and experiment-based efficacy prediction tools are primarily available for Streptococcus pyogenes Cas9 (SpCas9). One obstacle to develop such tools is the rarity of accurate data. Here, we report a method termed ‘Self-targeting sgRNA Library Screen’ (SLS) for assaying the activity of Cas9 nucleases in bacteria using random target/sgRNA libraries of self-targeting sgRNAs. Exploiting more than a million different sequences, we demonstrate the use of the method with the SpCas9-HF1 variant to analyse its activity and reveal motifs that influence its target-selectivity. We have also developed an algorithm for predicting the activity of SpCas9-HF1 with an accuracy matching those of existing tools. SLS is a facile alternative to the much more expensive and laborious approaches used currently and has the capability of delivering sufficient amount of data for most of the orthologs and variants of SpCas9. 相似文献
366.
Eszter A. Kish Claes-G?ran Granqvist András Dér Laszlo B. Kish 《Cognitive neurodynamics》2015,9(4):459-462
Even a single neuron may be able to produce significant lognormal features in its firing statistics due to noise in the charging ion current. A mathematical scheme introduced in advanced nanotechnology is relevant for the analysis of this mechanism in the simplest case, the integrate-and-fire model with white noise in the charging ion current. 相似文献
367.
László Zsolt Garamszegi Balázs Rosivall Sophie Rettenbacher Gábor Markó Sándor Zsebők Eszter Szöllősi Marcel Eens Jaime Potti János Török 《Ethology : formerly Zeitschrift fur Tierpsychologie》2012,118(7):621-635
Certain inherent characteristics of individuals can determine both physiological and behavioural responses to environmental challenges, which could drive a correlation between levels of corticosterone (CORT), the most important stress hormone and behavioural profiles. Therefore, CORT level may mediate consistent behaviours along the shy/bold continuum, and thus, it could serve as a pleiotropic basis for behavioural syndromes. Moreover, behavioural responses to environmental challenges may have consequences for CORT concentrations, which would also result in a correlation between physiology and behaviours even without requiring pleiotropic mechanisms. Accordingly, we investigated the relationship between CORT and behaviour in free‐living male collared flycatchers, Ficedula albicollis, using recently developed field assays. More specifically, we characterised novel object avoidance, intraspecific aggression and risk‐taking in males and related these correlated behaviours to the concentration of CORT metabolites in droppings measured by enzyme immunoassay. Individuals with higher levels of excreted CORT metabolites had no consistently higher or lower behavioural scores along the shy/bold spectrum, as avoidance of novelty, aggression and risk‐taking were not systematically related to CORT metabolite concentrations in the same direction. Moreover, environmental challenges owing to the presence of a novel object, territorial intruder and a potential predator caused no elevation in the level of CORT metabolites. Therefore, we did not find correlative evidence for CORT driving correlated behaviours through pleiotropic effects or for particular behaviours during courtship causing elevation in CORT levels. 相似文献
368.
Miklós Tóth István Ujváry Ivan Sivcev Zoltán Imrei István Szarukán Orsolya Farkas Ágnes Gömöry Eszter Gács-Baitz & Wittko Francke 《Entomologia Experimentalis et Applicata》2007,122(2):125-132
During our screening studies, attractiveness of a ternary mixture of synthetic Grandlure I [racemic cis‐1‐methyl‐2‐(1‐methylenethenyl)‐cyclobutane ethanol], Grandlure II [(Z)‐2‐(3,3‐dimethyl)cyclohexylidene ethanol], and Grandlure III–IV [(Z)‐ and (E)‐2‐ochtodenal; (Z)‐ and (E)‐(3,3‐dimethyl)cyclohexylidene acetaldehyde] for the sugar‐beet weevil, Bothynoderes punctiventris Germar (Coleoptera: Curculionidae), was observed in field‐trapping tests at several sites in Hungary and Serbia. The mixture attracted both males and females. Later tests revealed that of the components in the ternary mixture, only Grandlure III–IV were responsible for attraction, and the addition of Grandlures I or II in varying percentages had no influence on trap captures. Traps baited with 50–50 000 µg of Grandlure III–IV on rubber or polyethylene dispensers yielded high catches of weevils. When testing synthetic samples enriched in the respective geometrical isomer, Grandlure IV had a tendency of catching more weevils, but differences were not significant from lower catches by a 1:1 Z:E blend or Grandlure III. In gas chromatography–flame ionization detection/electroantennographic detection studies, antennae of both female and male weevils were more responsive to the (E)‐ than to the (Z)‐isomer suggesting a more important role for Grandlure IV. Efforts to verify the presence of Grandlure III or IV in volatiles collected from either sex of live sugar‐beet weevils or body washings with pentane remained inconclusive. Traps baited with Grandlure III–IV can now be used as sensitive and powerful trapping tools in the control of the sugar‐beet weevil. 相似文献