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排序方式: 共有194条查询结果,搜索用时 492 毫秒
91.
Jean-Nicolas Volff Yvonne Selz Carsten Hoffmann Alexander Froschauer Christina Schultheis Cornelia Schmidt Qingchun Zhou Wolfgang Bernhardt Reinhold Hanel Astrid B?hne Frédéric Brunet Béatrice Ségurens Arnaud Couloux Sylvie Bernard-Samain Valérie Barbe Catherine Ozouf-Costaz Delphine Galiana Martin J. Lohse Manfred Schartl 《Genetics》2013,195(4):1337-1352
92.
McFarlane L Svingen T Braasch I Koopman P Schartl M Wilhelm D 《Development genes and evolution》2011,221(2):95-104
AGO proteins are universal effectors of eukaryotic small RNA-directed regulatory pathways. In this study, we used a comparative
genomics approach to explore the AGO sub-family in the teleost clade. We identified five Ago homologues in teleost genomes, one more than encoded in other vertebrate clades. The additional teleost homologue was preserved
most likely due to the differential retention of regulatory elements following the fish-specific genome duplication event
that occurred approximately 350 million years ago. Analysis of all five Ago genomic loci in teleosts revealed that orthologues contain specific, conserved sequence elements in non-coding regions indicating
that the teleost Ago paralogues are differentially regulated. This was supported by qRT-PCR analysis that showed differential expression of the
zebrafish homologues across development and between adult tissues indicating stage and tissue-specific function of individual
AGO proteins. Multiple sequence alignments showed not only that all teleost homologues possess critical residues for AGO function,
but also that teleost homologues contain multiple orthologue-specific features, indicative of structural diversification.
Notably, these are retained throughout the vertebrate lineage arguing these may be important for orthologue-specific functions. 相似文献
93.
Lack of ultraviolet-light inducibility of the medakafish (Oryzias latipes) tumor suppressor gene p53 总被引:8,自引:0,他引:8
P53 is by far the most frequently altered gene in mammalian tumors. However, so far not a single p53 lesion has been reported in malignancies of cancer model systems in lower vertebrates. For analyzing the function of p53 in lower vertebrates, the gene was cloned from the medakafish (Oryzias latipes). Despite some differences in the genomic organization, the fish p53 amino acid sequence is highly conserved. Contrary to higher vertebrates, the level of p53 mRNA in medaka embryos gradually increases during embryogenesis. High expression of the p53 mRNA was detected in melanoma cells compared to undetectable expression of the gene in embryonic stem cells and fibroblasts. No effect of ultraviolet (UV) irradiation on the expression of p53 in cell cultures as well as in medaka fry was observed, indicating a possible difference in the function of p53 in lower vertebrates. 相似文献
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Herpin A Fischer P Liedtke D Kluever N Neuner C Raz E Schartl M 《Developmental biology》2008,320(2):319-327
Assembly and formation of the gonad primordium are the first steps toward gonad differentiation and subsequent sex differentiation. Primordial germ cells (PGCs) give rise to the gametes that are responsible for the development of a new organism in the next generation. In many organisms, following their specification the germ cells migrate toward the location of the prospective gonadal primordium. To accomplish this, the PGCs obtain directional cues from cells positioned along their migration path. One such cue, the chemokine SDF1 (stromal cell-derived factor 1) and its receptor CXCR4 have recently been found to be critical for proper PGC migration in zebrafish, chick and mouse.We have studied the mechanisms responsible for PGC migration in Medaka. In contrast to the situation observed in zebrafish, where proper PGC positioning is the result of active migration in the direction of the source of SDF1a, Medaka PGC movements are shown to be the consequence of a combination of active SDF1a and SDF1b-guided migration. In this process both SDF1 co-orthologues show only partly overlapping expression pattern and cooperate in the correct positioning of the PGCs. 相似文献
97.
Polyploidization is thought to be an important driving force in evolution as it increases the genetic material on which mutation and selection can act. In the Amazon molly, Poecilia formosa, triploid genotypes can be found in the field and frequently arise from diploid breeding stocks, a tetraploid individual, however, was so far never documented. Here, we report the first tetraploid Amazon molly. Flow cytometry clearly showed the tetraploid DNA content, whereas microsatellite analysis not only confirmed the tetraploidy but also pointed to allotetraploidy. Most likely the fourth genome was received through paternal leakage, namely, by fertilization of a triploid egg with a haploid sperm. The existence of tetraploid individuals offers new explanations for the enormous clonal diversity observed in wild populations of P. formosa. 相似文献
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Complexities of gene expression patterns in natural populations of an extremophile fish (Poecilia mexicana,Poeciliidae)
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