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21.
Phylogenomic species delimitation and host‐symbiont coevolution in the fungus‐farming ant genus Sericomyrmex Mayr (Hymenoptera: Formicidae): ultraconserved elements (UCEs) resolve a recent radiation 下载免费PDF全文
ANA JEŠOVNIK JEFFREY SOSA‐CALVO MICHAEL W. LLOYD MICHAEL G. BRANSTETTER FERNANDO FERNÁNDEZ TED R. SCHULTZ 《Systematic Entomology》2017,42(3):523-542
Ants in the Neotropical genus Sericomyrmex Mayr cultivate fungi for food. Both ants and fungi are obligate, coevolved symbionts. The taxonomy of Sericomyrmex is problematic because the morphology of the worker caste is generally homogeneous across all of the species within the genus, species limits are vague, and the relationships between them are unknown. We used ultraconserved elements (UCEs) as genome‐scale markers to reconstruct evolutionary history and to infer species boundaries in Sericomyrmex. We recovered an average of ~990 UCE loci for 88 Sericomyrmex samples from across the geographical range of the genus as well as for five outgroup taxa. Using maximum likelihood and species‐tree approaches, we recovered nearly identical topologies across datasets with 50–95% matrix completeness. We identify nine species‐level lineages in Sericomyrmex, including two new species. This is less than the previously described 19 species, even accounting for two species for which we had no UCE samples, which brings the total number of Sericomyrmex species to 11. Divergence‐dating analyses recovered 4.3 Ma as the crown‐group age estimates for Sericomyrmex, indicating a recent, rapid radiation. We also sequenced mitochondrial cytochrome oxidase subunit I (COI) for 125 specimens. Resolution and support for clades in our COI phylogeny are weak, indicating that COI is not an appropriate species‐delimitation tool. However, taxa within species consistently cluster together, suggesting that COI is useful as a species identification (‘DNA barcoding’) tool. We also sequenced internal transcribed spacer (ITS) and large subunit (LSU) for 32 Sericomyrmex fungal cultivars. The fungal phylogeny confirms that Sericomyrmex fungi are generalized higher‐attine cultivars, interspersed with Trachymyrmex‐associated fungal species, indicating cultivar sharing and horizontal transfer between these two genera. Our results indicate that UCEs offer immense potential for delimiting and resolving relationships of problematic, recently diverged species. 相似文献
22.
Kitty F Verzijlbergen Alex W Faber Iris JE Stulemeijer Fred van Leeuwen 《BMC molecular biology》2009,10(1):76
Background
Methylation of lysine 79 on histone H3 by Dot1 is required for maintenance of heterochromatin structure in yeast and humans. However, this histone modification occurs predominantly in euchromatin. Thus, Dot1 affects silencing by indirect mechanisms and does not act by the recruitment model commonly proposed for histone modifications. To better understand the role of H3K79 methylation gene silencing, we investigated the silencing function of Dot1 by genetic suppressor and enhancer analysis and examined the relationship between Dot1 and other global euchromatic histone modifiers. 相似文献23.
24.
Evolutionary dynamics of the kinetochore network in eukaryotes as revealed by comparative genomics 下载免费PDF全文
Jolien JE van Hooff Eelco Tromer Leny M van Wijk Berend Snel Geert JPL Kops 《EMBO reports》2017,18(9):1559-1571
During eukaryotic cell division, the sister chromatids of duplicated chromosomes are pulled apart by microtubules, which connect via kinetochores. The kinetochore is a multiprotein structure that links centromeres to microtubules, and that emits molecular signals in order to safeguard the equal distribution of duplicated chromosomes over daughter cells. Although microtubule‐mediated chromosome segregation is evolutionary conserved, kinetochore compositions seem to have diverged. To systematically inventory kinetochore diversity and to reconstruct its evolution, we determined orthologs of 70 kinetochore proteins in 90 phylogenetically diverse eukaryotes. The resulting ortholog sets imply that the last eukaryotic common ancestor (LECA) possessed a complex kinetochore and highlight that current‐day kinetochores differ substantially. These kinetochores diverged through gene loss, duplication, and, less frequently, invention and displacement. Various kinetochore components co‐evolved with one another, albeit in different manners. These co‐evolutionary patterns improve our understanding of kinetochore function and evolution, which we illustrated with the RZZ complex, TRIP13, the MCC, and some nuclear pore proteins. The extensive diversity of kinetochore compositions in eukaryotes poses numerous questions regarding evolutionary flexibility of essential cellular functions. 相似文献
25.
A general computational framework for modeling cellular structure and function. 总被引:4,自引:0,他引:4 下载免费PDF全文
The "Virtual Cell" provides a general system for testing cell biological mechanisms and creates a framework for encapsulating the burgeoning knowledge base comprising the distribution and dynamics of intracellular biochemical processes. It approaches the problem by associating biochemical and electrophysiological data describing individual reactions with experimental microscopic image data describing their subcellular localizations. Individual processes are collected within a physical and computational infrastructure that accommodates any molecular mechanism expressible as rate equations or membrane fluxes. An illustration of the method is provided by a dynamic simulation of IP3-mediated Ca2+ release from endoplasmic reticulum in a neuronal cell. The results can be directly compared to experimental observations and provide insight into the role of experimentally inaccessible components of the overall mechanism. 相似文献
26.
A large-subunit mitochondrial ribosomal DNA sequence translocated to the nuclear genome of two stone crabs (Menippe) 总被引:1,自引:0,他引:1
Two DNA sequences that appear to be homologous to large-subunit
mitochondrial ribosomal RNA genes have been identified in the stone crabs
Menippe mercenaria and M. adina. Amplification from whole genomic DNA by
polymerase chain reaction (PCR) with oligonucleotide primers based on
conserved portions of large-subunit mitochondrial rRNA genes consistently
amplified two products of similar length (565 and 567 bp). These products
differed at 3% of their nucleotide bases, and could be distinguished by a
HindIII site. Only one of these sequences (designated the A sequence) was
detected by PCR in purified mitochondrial DNA. The other (designated the B
sequence) hybridized to total genomic DNA at a level consistent with a
nuclear genome location. It is unlikely that the type B product would have
been recognized as a nuclear copy by examination of its sequence alone.
This is the first report of a mitochondrial gene sequence translocated into
the nuclear genome of a crustacean.
相似文献
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28.
D. A. Schaff G. A. Milliken C. D. Clayberg 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,74(4):538-541
Summary A diallel analysis is described for the case in which the values of the characteristic used in an inheritance study have unequal variances. Such a characteristic can be a mean, a slope of a regression line, or the estimates of some parameter of a linear or nonlinear model. Computational formulae are presented which incorporate the necessary weighting along with the statistics of the Hayman-Jinks method for diallel analysis. The method described can also be used to perform a weighted diallel analysis based on means when there are unequal numbers of replications per cross. A simple example demonstrates the computations necessary to complete a weighted diallel analysis.Journal paper no. 87-117-J of the Kansas Agricultural Experiment Station. This study was conducted by the senior author in partial fulfillment for the PhD degree in genetics 相似文献
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