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排序方式: 共有243条查询结果,搜索用时 31 毫秒
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Leo A Calvo-Bado Brian B Oakley Scot E Dowd Laura E Green Graham F Medley Atiya Ul-Hassan Vicky Bateman William Gaze Luci Witcomb Rose Grogono-Thomas Jasmeet Kaler Claire L Russell Elizabeth MH Wellington 《The ISME journal》2011,5(9):1426-1437
We report the first study of the bacterial microbiome of ovine interdigital skin based on 16S rRNA by pyrosequencing and conventional cloning with Sanger-sequencing. Three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. The sheep were classified as having either healthy interdigital skin (H) and interdigital dermatitis (ID) or virulent footrot (VFR). The ovine interdigital skin bacterial community varied significantly by flock and clinical condition. The diversity and richness of operational taxonomic units was greater in tissue from sheep with ID than H or VFR-affected sheep. Actinobacteria, Bacteriodetes, Firmicutes and Proteobacteria were the most abundant phyla comprising 25 genera. Peptostreptococcus, Corynebacterium and Staphylococcus were associated with H, ID and VFR, respectively. Sequences of Dichelobacter nodosus, the causal agent of ovine footrot, were not amplified because of mismatches in the 16S rRNA universal forward primer (27F). A specific real-time PCR assay was used to demonstrate the presence of D. nodosus, which was detected in all samples including the flock with no signs of ID or VFR. Sheep with ID had significantly higher numbers of D. nodosus (104–109 cells per g tissue) than those with H or VFR feet. 相似文献
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Dedmer B Van de Waal Jolanda MH Verspagen Jan F Finke Vasiliki Vournazou Anne K Immers W Edwin A Kardinaal Linda Tonk Sven Becker Ellen Van Donk Petra M Visser Jef Huisman 《The ISME journal》2011,5(9):1438-1450
Climate change scenarios predict a doubling of the atmospheric CO2 concentration by the end of this century. Yet, how rising CO2 will affect the species composition of aquatic microbial communities is still largely an open question. In this study, we develop a resource competition model to investigate competition for dissolved inorganic carbon in dense algal blooms. The model predicts how dynamic changes in carbon chemistry, pH and light conditions during bloom development feed back on competing phytoplankton species. We test the model predictions in chemostat experiments with monocultures and mixtures of a toxic and non-toxic strain of the freshwater cyanobacterium Microcystis aeruginosa. The toxic strain was able to reduce dissolved CO2 to lower concentrations than the non-toxic strain, and became dominant in competition at low CO2 levels. Conversely, the non-toxic strain could grow at lower light levels, and became dominant in competition at high CO2 levels but low light availability. The model captured the observed reversal in competitive dominance, and was quantitatively in good agreement with the results of the competition experiments. To assess whether microcystins might have a role in this reversal of competitive dominance, we performed further competition experiments with the wild-type strain M. aeruginosa PCC 7806 and its mcyB mutant impaired in microcystin production. The microcystin-producing wild type had a strong selective advantage at low CO2 levels but not at high CO2 levels. Our results thus demonstrate both in theory and experiment that rising CO2 levels can alter the community composition and toxicity of harmful algal blooms. 相似文献
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T. K. Rowles L. S. Schwacke R. S. Wells J. T. Saliki L. Hansen A. Hohn F. Townsend R. A. Sayre A. J. Hall 《Marine Mammal Science》2011,27(1):1-19
Cetacean morbilliviruses (CeMV) are viruses that can cause mass mortalities among various odontocete species. In this study levels of “herd” immunity in cetaceans from the U.S. coast are described from the distribution and prevalence of antibodies against morbilliviruses. Neutralizing antibody titers against dolphin morbillivirus (DMV), porpoise morbillivirus (PMV), phocine distemper (PDV), and canine distemper viruses (CDV) were measured. Positive samples had higher titers against the CeMV than against the other morbilliviruses tested, indicating that although PDV or CDV can be used to investigate exposure their use may result in a higher false negative rate. The results suggest that morbillivirus did not persist in coastal populations of bottlenose dolphins (Tursiops truncatus) after the major outbreaks that occurred in the 1980s and 1990s. Bottlenose dolphins from Beaufort, North Carolina; St. Joseph Bay, Florida; and Cape May, New Jersey had anti‐DMV seroprevalences ranging from between 15% and 33% but those from Charleston, South Carolina and Sarasota Bay, Florida, sampled in recent years were largely negative. These latter groups are therefore now vulnerable to infection and could experience high mortality if exposed to CeMV. Sero‐surveys of this kind are therefore vital for assessing the risk of new and recurring viral outbreaks in coastal cetaceans. 相似文献
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Soil water content, maize yield and its stability as affected by tillage and crop residue management in rainfed semi-arid highlands 总被引:3,自引:0,他引:3
Nele Verhulst Victoria Nelissen Niels Jespers Heleen Haven Ken D. Sayre Dirk Raes Jozef Deckers Bram Govaerts 《Plant and Soil》2011,344(1-2):73-85
Rainfed crop management systems need to be optimized to provide more resilient options to cope with projected climatic scenarios forecasting a decrease in mean precipitation and more frequent extreme drought periods in Mexico. Soil water content (0?C60 cm) was measured during three crop cycles in maize plots with different agronomic management practices in a long-term rainfed experiment (established in 1991) in the highlands of Mexico. Maize yields of 1997?C2009 were reported. Crop management practices varied in (1) tillage (conventional [CT] vs. zero tillage [ZT]) and (2) residue management (full or partial retention and removal). ZT with residue retention had higher soil water content than management practices involving CT and ZT with residue removal which provided a buffer for drought periods during the growing seasons. In 2009, a cycle with a prolonged drought during vegetative growth, this resulted in yield differences of up to 4.7 Mg ha?1 between ZT with (partial) residue retention and the other practices. Averaged over 1997?C2009, these practices had a yield advantage of approximately 1.5 Mg ha?1 over practices involving CT and ZT with residue removal. ZT with (partial) residue retention used rainfall more efficiently and resulted in a more resilient agronomic system than practices involving either CT or ZT with residue removal. 相似文献
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Various bacterial species produce and monitor low-molecular weight signaling molecules that regulate specific sets of genes in a population density-dependent manner. This process is known as quorum sensing (QS). To date, the detection of QS signaling molecules from Gram-negative bacteria has relied primarily on bacterial reporter strains. These bioassays are subject to substantial interference by compounds that affect the growth and metabolism of the reporter strains. In addition, the sensitivity of reporter strains to QS signaling molecules is population density-dependent. Here, we describe the development of an in vitro assay system for the rapid detection and quantification of the furanosyl borate diester (BAI-2) subclass of autoinducer 2 (AI-2), QS molecules. The sensor is based on ligand binding-induced changes in fluorescence resonance energy transfer (FRET) between a cyan and yellow variant of GFP fused to the termini of the BAI-2 receptor, LuxP. Unexpectedly, the addition of synthetic BAI-2 to the purified biosensor induces a decrease in the level of FRET between the terminal fluorophores. Several lines of evidence, including mutation of the ligand binding sites, indicate that the observed FRET changes are BAI-2-dependent. The FRET-based BAI-2 biosensor responded to the addition of culture filtrates from wild-type Vibrio harveyi but exhibited no response to culture filtrates from V. harveyi mutants defective in BAI-2 synthesis. The sensitivity of the biosensor to BAI-2 (apparent Kd = 270 nM) was similar to that of BAI-2 bioassay systems. The limitations of microbial bioassay systems and the advantages and potential applications for the FRET-based BAI-2 biosensor are discussed. 相似文献